Campylobacter And Listeria

Question 1

Reservoir for campylobacter jejuni/coli

Answer 1

Poultry
Cattle
Pigs
Horses
Rabbits
Birds
Other animals

Question 2

How much O2 and CO2 are required for campylobacter growth ?

Answer 2

Small amount of O2 (3-6%)
10% Co2

Campylobacter is microaerophilic

Question 3

Chronic sequence that are caused by campylobacter

Answer 3

Reactive arthritis

Guillain Barre syndrome (acute flaccid paralysis )

Question 4

Most commonly reported bacterial cause of foodborne illness in the US

Answer 4

Campylobacter

Question 5

What are the reservoirs for Campylobacter jejuni

Answer 5

Rabbits 
Rodents 
Birds 
Sheep
Horses 
Cows 
Pigs 
Poultry ***
Many animals

Question 6

Outbreaks in the cause of Campylobacter jejuni is

Answer 6

Rare and most cases are sporadic

Question 7

70% of cases of Campylobacter jejuni disease is

Answer 7

Consumption of contaminated or improperly cooked poultry

Question 8

Minimum temp for growth of C.jejuni

Answer 8

30 C

Question 9

C.jejuni is able to grow at

Answer 9

42 C

Question 10

C.jejuni survival outside of the gut is

Answer 10

Poor

Question 11

Campylobacteriosis incubation time

Answer 11

2-5 days

Question 12

Campylobacteriosis duration

Answer 12

7-10 days ( relapses 25%)

Question 13

Campylobacteriosis infectious dose

Answer 13

100-500 cells

Question 14

Campylobacteriosis mortality rate

Answer 14

Rare <0.03%

Question 15

Campylobacteriosis symptoms

Answer 15

Diarrhea
Fever
Abdominal cramping
Sometimes : bloody diarrhea, nausea, vomiting

Question 16

Campylobacteriosis less frequently symptoms

Answer 16

Bacteremia( mostly in immuno compromised)
Septic arthritis
Extra intestinal symptoms

Question 17

Pathogenicity of Campylobacteriosis

Answer 17

Can result in eterotoxigenic like illness with watery diarrhea

Can result in inflammatory colitis with fever and fecal blood and occasionally bacteremia that suggests an invasive mechanism

Question 18

Virulence factor for C. Jejuni

Answer 18

Health of host 
Age of host 
Flagella aid in attachment followed by
Host cell invasion
Toxin production 
Epithelial cell disruption and leakage

Question 19

Complications of C.Jejuni

Answer 19

Arthritis 
Carditis, endocarditis 
Meningitis 
Septicemia 
GBS

Question 20

What is GBS

Answer 20

Acute demyelination polyneuropathy Rapid onset of numbness,pain, and progressive weakness or paralysis

Question 21

How many percent of GBS patients had C.Jejuni

Answer 21

20-40%

Question 22

GBS attributes to autoimmune response to

Answer 22

Bacterial N-acetyl glucosamine antigens

Question 23

GBS has core oligosacharides structure that mimics

Answer 23

Ganglioside structures of the motor neurons

Question 24

Listeria monocytogenes is gram

Answer 24

+

Question 25

Listeria monocytogenes is psychotropic that means

Answer 25

They can grow in cold environment

Question 26

Listeria monocytogenes is a zero tolerance in

Answer 26

Ready to eat foods

Question 27

How many serotypes does Listeria monocytogenes have

Answer 27

13

Question 28

The most common LM serotypes that causes human disease

Answer 28

4b

Question 29

Symptoms of listeriosis

Answer 29

Flu like symptoms

Question 30

Complications for listeriosis

Answer 30

``` Meningitis Miscarriage Stillbirth Septicemia Mild gastroenteritis ```

Question 31

Why is listeriosis atypical foodborne disease ? Why is it different than other foodborne diseases

Answer 31

``` Severity and non-enteric nature High fatality rate ( 20-30%) Long incubation time (4d to weeks ) High risk of individuals who have underlying problems that affect T cell mediated immunity Manifestations are host dependent ```

Question 32

Mortality rate of listeriosis in immunocompromised and elderly

Answer 32

20-40%

Question 33

Clinical symptoms of listeriosis in healthy adults

Answer 33

GI symptoms

Question 34

Clinical symptoms of listeriosis in pregnant women

Answer 34

``` Fever Muscle aches Diarrhea Premature delivery Still birth ```

Question 35

Clinical symptoms of listeriosis in newborns

Answer 35

Meningitis Pneumonia Fever

Question 36

Clinical symptoms of listeriosis in immunocompromised adults

Answer 36

Meningitis, blood infection

Question 37

Virulent listeria species

Answer 37

Listeria monocytogenes and | Listeria ivanovii

Question 38

This species of listeria is a foodborne concern

Answer 38

Listeria myocytogenes

Question 39

What conditions can predispose adults to complications of listeriosis

Answer 39

``` Neoplasm Aids Alcoholism Pregnancy Diabetes CVD Renal transplant Corticosteroid therapy ```

Question 40

LM bacteria can attach to host cells by

Answer 40

Internalins Proteins that bind specifically to host cell

Question 41

What does LM produce in order to scape endosome and enter the cytoplasm ?

Answer 41

Listerolysin O (LLO)

Question 42

LM uses -----to move about the cell and into adjoins cells

Answer 42

Actin

Question 43

C. Jejuni is capnophilic which means

Answer 43

Thrive in high concentration of CO2 | 2-10%

Question 44

Symptoms of Listeriosis in pregnant women

Answer 44

``` Flu like symptoms Abortion Fetal death Still birth Severe neonatal septicemia Meningitis ```

Question 45

LM bacteria adhesion by

Answer 45

Internalins Proteins that bind specifically to host cells

Question 46

Tetracycline resistant L monocytogenes from animal product may have obtained the tet gene from

Answer 46

Other gram positive bacteria in animal GI tract