C3: Chemistry And Hematology Analyzers

Question 1

Fibrin

Answer 1

Clotting factors, blood clots when it hits an abnormal surface

Question 2

Serum

Answer 2

Fluid portion of blood, no clotting factors

Question 3

Plasma

Answer 3

Fluid portion on blood, clotting factors remain

Question 4

Whole Blood

Answer 4

Blood with anticoagulants that has no been spun down

Question 5

Chemistry Analyzer

Answer 5

• serum
• evaluates: internal organ function, hydration status, acid/base balance, blood-glucose level
• technology: photometry, electrochemical, chemical reagents

Question 6

Hematology Analyzer

Answer 6

• whole blood (cellular portion of blood)
• evaluates: level or red blood cells, number and type of white blood cells, clotting ability, info on health of individual cells
• technology: impedance, laser based, quantitative buffy coat, fluorescence technology

Question 7

ELISA Technology

Enzyme Linked Immunosorbent Assay

Answer 7

• snap test
• detects specific antigen
• tests for: heartworm, FeLV, FIV, canine parvovirus, progesterone, bile acids (liver function), T4 (kidney function), cortisol, canine lipase (pancreatitis), giardia, combination tests

Question 8

Red Tinge Serum/Plasma

Hemolysis

Answer 8

• red blood cells have ruptured

- caused by too much pressure on syringe, forcing blood through a small needle, vigorous mixing, stressful collection

Question 9

Yellow/Orange Tinged Serum/Plasma

Icterus

Answer 9

Caused by hepatic disorders, bilirubin released in blood

Question 10

Cloudy Tinged Serum/Plasma

Lipemia

Answer 10

Related to recent intake of food

Question 11

PVC

Answer 11

• packed cell volume

- microhematicrit tube

Question 12

TP

Answer 12

• total protein

- read plasma on refractometer

Question 13

Chemistry Analyzer

Photometry

Answer 13

Uses differences in light to generate results

Question 14

Chemistry Analyzer

Electrochemical

Answer 14

Uses differences in voltage or electric potential to generate results

Question 15

Chemistry Analyzer

Chemical Reagents

Answer 15

Use chemical reactions to generate results

Question 16

Hematology Analyzers

Impedance

Answer 16

Uses passage of electrical current across two electrodes to count cells and determine size differences

Question 17

Hematology Analyzers

Laser-Based

Answer 17

Laser beams use light scatter differences between cell types

Question 18

Hematology Analyzers

Quantitative Buffy Coat

Answer 18

Uses a centrifuge technique to separate cells into different layers based on their density

Question 19

Hematology Analyzers

Fluorescence Technology

Answer 19

Uses a fluorescent dye that stains different cell components, differences in cell fluorescence is detected by the optics of the analyzer