(C) Reagents and Supplies Flashcards
Containers and receivers are not calibrated to hold…
an exact volume.
What is the use of beakers?
General mixing and reagent prep.
What is the use of Erlenmeyer flasks?
For preparing reagents and titration procedures.
Where would you dispose of a test tube that is contaminated with blood?
Sharps container.
What type of glassware ensures volume being delivered or held is actually that volume.
Volumetric glassware.
What agency produces standards for glassware calibration specifications?
National Institute of Standards and Technology (NIST).
Describe the difference between a volumetric flask and a graduated measuring cylinder.
Volumetric flask: tapered neck, round bulb at the bottom; to contain.
Graduated Measuring Cylinder: measure liquids when high accuracy is not needed; to deliver.
TC pipettes (should/should not) be blown out.
TC pipettes should be blown out.
TD pipettes (should/should not) be blown out.
TD pipettes should not be blown out.
A single ring on a pipette indicates…
should not be blown out.
A double ring on a pipette indicates…
should be blown out.
Describe Mohr/Graduated pipettes.
Graduation stops above the top end; NOT blown out.
Describe Serological pipettes.
Graduation to tip end; blown out to deliver all liquid.
Describe Ostwald-Folin pipettes.
Bulb at the lower end of the pipette, one fixed volume; blow-out mandatory; used for viscous solutions (blood, serum).
Describe Volumetric pipettes.
Bulb in the middle of the pipette, one fixed volume; NOT blown out; only for aqueous solution.
Describe the procedure of manual pipette use.
(1) Use mechanical suction
(2) Wipe off outside of pipette with gauze
(3) Adjusting the meniscus
(4) Drain into receiving vessel
Set Micropipettors are TD or TC?
TD.
Type I purity of water is define as…
The purest type; commonly used with electrophoresis, HPLC, Mass Spec, and PCR testing; must be used right away.
Type II purity of water is define as…
Used in most lab procedures.
Type III purity of water is define as…
A few general lab tests; heating baths; often used to make Type I or Type II.
Distilled water is described as…
Removes minerals and microbiological organisms; still contains impurities (CO2, chlorine, ammonia) but meets the specs for Type II and III water.
Describe how distilled water is made.
Water is boiled; steam is cooled and condensed; product is distilled water.
Describe how deionized water is made.
Water is passed through a resin column that are positive and negatively charged that bind to any charged molecules and removes them.
Deionized water can also be treated with what two techniques to produce Type I water?
Membrane filtration or activated charcoal.
Reverse osmosis removes almost everything and is considered what type of water?
Type III water.
Describe how RO water is made.
Water is placed under pressure and passed through a semipermeable membrane.
What types of water is approved by CLSI as Clinical Laboratory reagent water?
Type I or Type II water.
Rate the grades of purity from highest purity to lowest purity.
ACS > Reagent > USP > NF > Laboratory > Purified > Technical.
What grade of purity is the most commonly used in the laboratory?
ACS, reagent grade, and laboratory grade.
