(C) Reagents and Supplies Flashcards

1
Q

Containers and receivers are not calibrated to hold…

A

an exact volume.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

What is the use of beakers?

A

General mixing and reagent prep.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

What is the use of Erlenmeyer flasks?

A

For preparing reagents and titration procedures.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

Where would you dispose of a test tube that is contaminated with blood?

A

Sharps container.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

What type of glassware ensures volume being delivered or held is actually that volume.

A

Volumetric glassware.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

What agency produces standards for glassware calibration specifications?

A

National Institute of Standards and Technology (NIST).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

Describe the difference between a volumetric flask and a graduated measuring cylinder.

A

Volumetric flask: tapered neck, round bulb at the bottom; to contain.

Graduated Measuring Cylinder: measure liquids when high accuracy is not needed; to deliver.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

TC pipettes (should/should not) be blown out.

A

TC pipettes should be blown out.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

TD pipettes (should/should not) be blown out.

A

TD pipettes should not be blown out.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

A single ring on a pipette indicates…

A

should not be blown out.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

A double ring on a pipette indicates…

A

should be blown out.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

Describe Mohr/Graduated pipettes.

A

Graduation stops above the top end; NOT blown out.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

Describe Serological pipettes.

A

Graduation to tip end; blown out to deliver all liquid.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

Describe Ostwald-Folin pipettes.

A

Bulb at the lower end of the pipette, one fixed volume; blow-out mandatory; used for viscous solutions (blood, serum).

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

Describe Volumetric pipettes.

A

Bulb in the middle of the pipette, one fixed volume; NOT blown out; only for aqueous solution.

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

Describe the procedure of manual pipette use.

A

(1) Use mechanical suction
(2) Wipe off outside of pipette with gauze
(3) Adjusting the meniscus
(4) Drain into receiving vessel

17
Q

Set Micropipettors are TD or TC?

18
Q

Type I purity of water is define as…

A

The purest type; commonly used with electrophoresis, HPLC, Mass Spec, and PCR testing; must be used right away.

19
Q

Type II purity of water is define as…

A

Used in most lab procedures.

20
Q

Type III purity of water is define as…

A

A few general lab tests; heating baths; often used to make Type I or Type II.

21
Q

Distilled water is described as…

A

Removes minerals and microbiological organisms; still contains impurities (CO2, chlorine, ammonia) but meets the specs for Type II and III water.

22
Q

Describe how distilled water is made.

A

Water is boiled; steam is cooled and condensed; product is distilled water.

23
Q

Describe how deionized water is made.

A

Water is passed through a resin column that are positive and negatively charged that bind to any charged molecules and removes them.

24
Q

Deionized water can also be treated with what two techniques to produce Type I water?

A

Membrane filtration or activated charcoal.

25
Reverse osmosis removes almost everything and is considered what type of water?
Type III water.
26
Describe how RO water is made.
Water is placed under pressure and passed through a semipermeable membrane.
27
What types of water is approved by CLSI as Clinical Laboratory reagent water?
Type I or Type II water.
28
Rate the grades of purity from highest purity to lowest purity.
ACS > Reagent > USP > NF > Laboratory > Purified > Technical.
29
What grade of purity is the most commonly used in the laboratory?
ACS, reagent grade, and laboratory grade.