(C) Reagents and Supplies

Question 1

Containers and receivers are not calibrated to hold…

Answer 1

an exact volume.

Question 2

What is the use of beakers?

Answer 2

General mixing and reagent prep.

Question 3

What is the use of Erlenmeyer flasks?

Answer 3

For preparing reagents and titration procedures.

Question 4

Where would you dispose of a test tube that is contaminated with blood?

Answer 4

Sharps container.

Question 5

What type of glassware ensures volume being delivered or held is actually that volume.

Answer 5

Volumetric glassware.

Question 6

What agency produces standards for glassware calibration specifications?

Answer 6

National Institute of Standards and Technology (NIST).

Question 7

Describe the difference between a volumetric flask and a graduated measuring cylinder.

Answer 7

Volumetric flask: tapered neck, round bulb at the bottom; to contain.

Graduated Measuring Cylinder: measure liquids when high accuracy is not needed; to deliver.

Question 8

TC pipettes (should/should not) be blown out.

Answer 8

TC pipettes should be blown out.

Question 9

TD pipettes (should/should not) be blown out.

Answer 9

TD pipettes should not be blown out.

Question 10

A single ring on a pipette indicates…

Answer 10

should not be blown out.

Question 11

A double ring on a pipette indicates…

Answer 11

should be blown out.

Question 12

Describe Mohr/Graduated pipettes.

Answer 12

Graduation stops above the top end; NOT blown out.

Question 13

Describe Serological pipettes.

Answer 13

Graduation to tip end; blown out to deliver all liquid.

Question 14

Describe Ostwald-Folin pipettes.

Answer 14

Bulb at the lower end of the pipette, one fixed volume; blow-out mandatory; used for viscous solutions (blood, serum).

Question 15

Describe Volumetric pipettes.

Answer 15

Bulb in the middle of the pipette, one fixed volume; NOT blown out; only for aqueous solution.

Question 16

Describe the procedure of manual pipette use.

Answer 16

(1) Use mechanical suction
(2) Wipe off outside of pipette with gauze
(3) Adjusting the meniscus
(4) Drain into receiving vessel

Question 17

Set Micropipettors are TD or TC?

Answer 17

TD.

Question 18

Type I purity of water is define as…

Answer 18

The purest type; commonly used with electrophoresis, HPLC, Mass Spec, and PCR testing; must be used right away.

Question 19

Type II purity of water is define as…

Answer 19

Used in most lab procedures.

Question 20

Type III purity of water is define as…

Answer 20

A few general lab tests; heating baths; often used to make Type I or Type II.

Question 21

Distilled water is described as…

Answer 21

Removes minerals and microbiological organisms; still contains impurities (CO2, chlorine, ammonia) but meets the specs for Type II and III water.

Question 22

Describe how distilled water is made.

Answer 22

Water is boiled; steam is cooled and condensed; product is distilled water.

Question 23

Describe how deionized water is made.

Answer 23

Water is passed through a resin column that are positive and negatively charged that bind to any charged molecules and removes them.

Question 24

Deionized water can also be treated with what two techniques to produce Type I water?

Answer 24

Membrane filtration or activated charcoal.

Question 25

Reverse osmosis removes almost everything and is considered what type of water?

Answer 25

Type III water.

Question 26

Describe how RO water is made.

Answer 26

Water is placed under pressure and passed through a semipermeable membrane.

Question 27

What types of water is approved by CLSI as Clinical Laboratory reagent water?

Answer 27

Type I or Type II water.

Question 28

Rate the grades of purity from highest purity to lowest purity.

Answer 28

ACS > Reagent > USP > NF > Laboratory > Purified > Technical.

Question 29

What grade of purity is the most commonly used in the laboratory?

Answer 29

ACS, reagent grade, and laboratory grade.