Bonus Flashcards
If scientists are interested in comparing variation in body color between the honey bee (Apis mellifera) and the European hornet (Vespa crabro), what type of variation are they studying?
Interspecific variation
If genes are linked, we expect to see a greater number of nonrecombinant gametes than recombinant gametes.
True
What does it mean to backcross?
The F1 individuals will be crossed with a fly of the parental type
Which of the following is a true statement:
Male fruit flies do not generally have crossover events when making gametes
Standard deviation is:
how spread out the data is
the square root of the variance
Two genes that are far apart are more likely to crossover than 2 genes close together
True
A SNP marker is found linked to the cystic fibrosis gene. Cystic fibrosis is an autosomal recessive disease. A couple plans to have children together and both are carriers for the cystic fibrosis gene but do not have the disease themselves. They are both A1/A2 at the SNP and the A1 allele is linked with the allele causing cystic fibrosis. Assuming no crossing over between the SNP and the disease gene, what genotypes in the offspring could result that would cause cystic fibrosis?
A1 – cf, A1 – cf
The genes responsible for albinism (A or a) and pink eyes (P or p) in mice are linked and wild-type pigmentation is dominant in both traits. A homozygous wild-type mouse is mated with an albino, pink-eyed mouse. The resulting F1 mice are crossed with albino, pink-eyed mice. The F2 progeny of this testcross are:
wild-type body color, wild-type eyes 22
albino body color, wild-type eyes 7
wild-type body color, pink eyes 3
albino body color, pink eyes 18
How many cM apart are the albinism and pink-eye genes?
20
A lac operon of genotype I+ P- O+ Z- Y+ will produce permease when:
it won’t produce permease because the promoter is mutated.
What are the products of an operon after it is transcribed and translated?
A single mRNA transcript which is translated into multiple proteins
What pattern of functional protein synthesis of β-galactosidase and permease, respectively, would you expect from an I+P+OCZ+Y+ lac operon in the absence of lactose?
++
Where would the repressor be bound in an E. coli cell (nonmutant) that is growing in lactose?
the repressor would not be bound
For your 25ul ligation reaction: You need to add 160ng of your digested insert DNA (which is at a concentration of 160ng/ul) and 25ng of your digested plasmid DNA (which is at a concentration of 10ng/ul) to your ligation reaction. You also want 1X ligase buffer (it is provided at a 10X concentration) and 1ul ligase. How much water will you need to add to the reaction?
18ul
Where would the repressor be bound in an E. coli cell (nonmutant) that is NOT growing in lactose?
O,
The M13F/M13R primers you used to PCR amplify your human genomic DNA insert from your bacterial colony in your 5-week project were designed to complement/bind/anneal:
Plasmid Puc19
