Body Sites (stool) Flashcards

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1
Q

What is an infant’s gastrointestinal tract colonized with?

A

Normal skin flora

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2
Q

Which types of bacteria are most commonly found in the distal ileum of the GI tract?

A

Enterics, Bacteroides spp.

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3
Q

Which is the most sparsely populated area if the GI tract?

A

The upper small intestine

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4
Q

In the adult large bowel, by how much do anaerobes outnumber aerobes?

A

1000 to 1

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5
Q

T or F? Worldwide, diarrheal disease is 2nd leading cause of death.

A

True

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6
Q

How is diarrheal disease most often transmitted?

A

By the fecal/oral route or contaminated food/water

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7
Q

T or F? Acute diarrheal syndromes are not usually self-limiting

A

False, they usually are.

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8
Q

What are some factors of host defense against GI pathogens?

A

Acidity of stomach
Normal peristalsis
Mucous layer coating the epithelium (GALT)
Normal flora, preventing colonization by potential pathogens

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9
Q

What are some pathogenic microbial factors of GI pathogens?

A

Toxins: enterotoxins, cytotoxins, and neurotoxins
Attachment mechanisms
Invasion of the host’s intracellular environment

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10
Q

What are the 4 most common bacteria that cause food poisoning by intoxication?

A

Staph aureus, Bacillus cereus, Type A. Clostridium perfringens (watery diarrhea) and Clostridium botulinum

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11
Q

What type of diarrhea is described? Fluid & electrolyte balance upset
No Fecal Leukocytes present

A

Watery diarrhea

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12
Q

What type of diarrhea is described? Cell destruction and inflammation occur
Fecal Leukocytes present
Mucus and blood present

A

Bloody Diarrhea (Dysentery)

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13
Q

What are the three organisms most commonly found in routine stool culture that may cause watery diarrhea?

A

Aeromonas, Vibrio cholera, and Yersinia enterocolitica

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14
Q

What are the five organisms most commonly found in routine stool culture that may cause bloody diarrhea?

A

Aeromonas, Campylobacter spp., Plesiomonas shigelloides, Salmonella spp., and Shigella spp.

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15
Q

Which type of E. coli is most like Shigella?

A

Enteroinvasive E. coli (EAEC)

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16
Q

Which type of E. coli is known to cause traveler’s diarrhea?

A

Enterotoxigenic E. coli (ETEC)

17
Q

What type of E. coli is E. coli O157:H7?

A

Enterohemorrhagic E.coli (STEC)

18
Q

What is a wet mount usually used for?

A

For detection of motile parasites (in a fresh liquid specimen)

19
Q

What do we use to detect E.coli O157:H7, shiga toxins, and Clostridium difficile toxins A or A/B?

A

Antigen detection - EIA

20
Q

Supports the growth of most yeasts and bacteria

A

Blood agar

21
Q

Supports the growth of enterics and vibrios

A

MAC

22
Q

Used for E coli O157:H7 (NSF)

A

MAC + sorbitol

23
Q

Moderately selective for Salmonella and Shigella

A

HE (or XLD)

24
Q

Selective media with the following incubation conditions: 42°C in 5% O2, 10% CO2, 85% N2 (microaerophilic)

A

Campy agar (CVA) - selective for Campylobacter

25
Q

Incubation conditions for BAP, MAC, MAC-Sorb, HE

A

35-37°C in ambient air; hold 48 hours

26
Q

How long does CVA agar incubate for, what does it recover?

A

72h, Campy

27
Q

If a Yersinia culture is requested, how would you plate it and grow it?

A

Room Temp (25C°) BAP+MAC + 35-37°C BAP+MAC