Body Fluids and Electrolytes Flashcards
What are electrolytes?
Molecules that dissociate into charged ions in water, carry electrical current
Anions or cations
What are anions?
Negatively charged ions
Move to the anode (positive pole)
What are examples of anions?
Chloride
Bicarbonate
Phosphate
What are cations?
Positively charged ions
Move to the cathode (negative pole)
What are examples of cations?
Sodium
Potassium
Magnesium
Calcium
What are the functions of electrolytes?
Maintain osmotic pressure and water balance Maintain pH Regulate heart and muscle function Redox reactions Enzyme cofactors/activators
What interference should be avoided for electrolyte analysis? Why?
Hemolysis
Increased K
Decreased Na and Cl by dilution
Why should serum/plasma be separated from cells quickly?
Otherwise K will be released into plasma and falsely increase results
What might cause falsely increased K before collection?
Exercise
Pumping fist
Hemoconcentration due to extended tourniquet use
How is water distributed in the body?
Intracellular fluid (70%)
Extracellular fluid (30%)
- Plasma (20%)
- Interstitial fluid (80%)
How does osmosis work?
Water moves across semi-permeable membranes into the compartment with a higher concentration of non-diffusible particles
How is water control mainly achieved by the kidneys?
By ADH
What happens during a concentrated state?
Hypernatremia
Hyperosmolality (more solutes)
Hypovolemia (low water volume)
What happens during a diluted state?
Hyponatermia
Hyposmolality (less solutes)
Hypervolemia (high water volume)
How is water balance monitored?
CNS osmoregulators
Baroreceptors in kidneys
Baroreceptors in heart and blood vessels
Adrenal cortical cells
How does aldosterone help water regulation?
Increases sodium resorption and potassium excretion during hyponatremia/hyposmolality
Decreases sodium resorption and potassium excretion during hypernatremia/hyperosmolality
How does ADH help water regulation?
When osmolality rises ADH is released stimulating the resorption of water to dilute the blood
When osmolality lowers ADH is suppressed in order to secrete excess water
What is sodium?
Dominant electrolyte in ECF
Major extracellular cation
What does sodium do?
Major role in plasma osmolality and water balance
Where is sodium high? Low?
Very low in cells due to Na/K pump
High in ECR
How are sodium levels regulated?
Filtered by kidneys then reabsorbed as controlled by aldosterone
What stimulates aldosterone release? From where?
Released from the adrenal cortex in response to:
Low osmolality
Low sodium
High potassium
Low cardiac output
What is potassium?
Major intracellular cation
Where is potassium high? Low?
High in cells
Low in fluids
How are potassium levels regulated?
Filtered by kidneys then reabsorbed the excreted again
How does aldosterone affect potassium levels?
Lowers potassium
Increase sodium resorption means potassium excreted in exchange
How might potassium be lost from cells?
Dehydration - follows water out Acidosis - displaced by H+ Cellular breakdown (crush injuries, protein breakdown)
How might potassium be gained in cells?
Alkalosis - moves into cell as H+ leaves
What does potassium do?
Regulating cell membrane potentials (neuromuscular excitability)
What happens if potassium is imbalanced?
Cardiac arrythmias
Muscle weakness
What is chloride?
Major extracellular anion
How are chloride levels regulated?
Filtered by kidneys the reabsorbed
Follows sodium passively and “pumped” back in
What does chloride do?
Maintains electroneutrality
Retained if cations are increased
Lost if anions are increased
What is the anion gap formula?
Na - [Cl + HCO3]
or
[Na + K] - [Cl + HCO3]
What causes the anion gap?
Anions in the sample not measured such as albumin, proteins, phosphate, etc
What causes an increased anion gap?
Displacement of Cl by other anions
Low Cl is not accounted for by an increase in HCO3 or decrease in cations
What are examples of things that cause an increased anion gap?
Ketones
Lactic acid
Toxic ingestions (alcohol, salicylate)
Is a decreased anion gap possible?
Not really, maybe hypoalbuminemia
Usually test issue
What is bicarbonate?
Major buffer base of plasma
The form most CO2 is transported as in plasma
How are bicarbonate levels controlled?
Filtered by kidneys
Converted to carbonic acid and CO2
CO2 enters cells and is used to create new HCO3 when it moves into the blood
What is magnesium?
Second most abundant intracellular cation
What does magnesium do?
Enzyme activator
Influences nerve control
Influences neuromuscular contration
Formation of bones and teeth
Where is most magnesium found?
65% in bones and teeth
30% in intracellular fluid
How is magnesium found in blood?
30% protein bound
Ionized form is active
How is magnesium regulated?
PTH regulates plasma levels by increasing resorption
Where is calcium found in the body?
99% bones and teeth
How does calcium travel in the blood?
50% free, ionized, active
45% bound mostly to albumin
5% in complexes
What is ionized calcium levels dependent on?
Dependent on plasma protein and pH levels
Reduced in alkalosis and when there is increased plasma proteins
What does calcium do?
Enzyme activator (coagulation)
Muscle contraction
Influences membrane permeability
Influences cell motility
What happens when calcium is elevated?
Muscle weakness
Loss of neuromuscular excitability
What happens when calcium is decreased?
Increased muscle excitability
Spasms
Cardiac arrhythmia
How is calcium absorbed into the body?
Requires activated vitamin D in intestine
What causes decreased calcium absorption?
High pH
High phosphate level
How is calcium regulated?
PTH and calcitonin
How does PTH affect calcium blood levels?
PTH is secreted when calcium is low
It increases reabsorption by kidneys and breakdown of bone by osteoclasts
How does calcitonin affect calcium blood levels?
Calcitonin is secreted when calcium is high
It decreases kidney reabsorption and simulates osteoblasts to increase bone synthesis (inhibits osteoclasts)
What is phosphorus?
Major intracellular anion
Component of many molecules like ATP
Where is most phosphorus found?
80% in bones
How is phosphorus absorbed?
Vitamin D increases absorption and kidney resorption
What affects phosphorus levels the most?
PTH
Which decreases phosphorus by increasing excretion
What acts conversely with calcium?
Phosphorus
Which electrolytes are mostly in ECF?
Sodium and chloride
Which electrolytes are mostly in ICF?
Potassium, magnesium, and phosphorus
What is fluid depletion?
Loss of fluid
What causes fluid depletion?
Vomiting/diarrhea
Burns
Decreased intake
Sweating/high temperature
What are electrolyte findings in water depletion?
Increased sodium and osmolality
Decreased urine volumeW
What is diabetes insipidus?
Decreased ADH causes less water to be reabsorbed by kidneys
Urine is very dilute but large quantities
What is fluid excess?
Increased fluid
What causes fluid excess?
Failure of homeostasis
Increased intake
Increased ADH
What can fluid excess cause?
Edema, accumulation of interstitial fluid causing swelling
What causes hypernatremia?
Decreased plasma water
or
Increased plasma Na
When might hypernatremia be seen?
Dehydration
Diabetes insipidus
Primary hyperaldosteronism (increased resorption of Na)
Excess intake
What causes hyponatremia
Increased plasma water
or
Decreased plasma Na
When might hyponatremia be seen?
Diuretic medication (decreased resorption) Excessive sweating Renal conditions Hypoaldosteronism/Addison's Disease Severe vomiting and diarrhea Acidosis Decreased intake Syndrome of inappropriate ADH secretion (water retention)
What is pseudohyponatremia?
Electrolyte exclusion effect
Increased protein or lipid reduce water component
Apparent decrease in all electrolytes using indirect methods (dilution)
What is hyperkalemia?
Elevated potassium
What causes hyperkalemia?
Crush injuries Metabolic acidosis (pH decreases K increases due to displacement from cells and H+ excreted over K) Hypoaldosteronism/Addison's Disease (Na secreted over K)
What causes hypokalemia
Hyperaldosteronism (Na reabsorbed, K excreted) Metabolic alkalosis (K excreted over H+) Vomitting/diarrhea