Blood Sampling & Examination Flashcards
Normal ph of blood
7.3-7.5
Collection and storage of serum samples
Fill to line! Plain tube - kept out of sunlight; takes 2hrs to clot Serum gel tube - centrifuged once clotted Storage - 2-8c for a few days - -20c for longer
Collection and storage of plasma samples
Fill to line! Anticoagulant tube Centrifuged soon after collection Storage - 2-8c few days - -20c for longer
EDTA tube - full name
Colours
Use
Ethylene diamine tetra acetic acid
Pink tube / lilac vac
Haematology
Lithium heparin tube
Colours
Use (2)
Orange tube / green vac
Biochemistry
Hormone tests
Sodium fluoride / potassium oxalate
Colours
Use
Yellow tube / grey vac
Glucose
Sodium/lithium citrate
Colours
Use
Blue tube/vac
Coagulation profiles
Tubes with no anticoagulant
Colours
Use
White/brown tube / red vac
Serum collection
Reasons for lipaemia
What does it interfere with
Recent feeding; hepatic disease
Serum biochem
Define icterus
What does it indicate
High levels of bilirubin; yellow appearance
Indicates hepatic disease
What is haematocrit used to assess
Packed cell volume
Anaemia/haemorrhage/dehydration
What may an increased haematocrit indicate (3)
Dehydration
Endotoxic shock
Splenic issues
What may a decreased haematocrit indicate (2)
Anaemia
Haemorrhage
In what breed may an there may an abnormal haematocrit which is considered normal and why
Greyhounds
Have significantly more rbc
Normally higher higher PCV
What is blood smear used to examine and identify
Red and white blood cells
- size/shape/colour/total count
- abnormalities
- diff. WBC counts
How should a blood smear be examined
Scan w/low mag for count
Oil immersion for more detailed exam
How long does it take for normal blood to clot
What are coagulation profiles used to test for (2)
Within 1-2mins
Von willebrands
Warfarin poisoning
Blood and microhaematocrit centrofuging
- speed and time
Blood - 10,000 rpm
Microhaematocrit - pre-set speed
5 mins for both
Needle size for blood sampling
21-23G
Larger the better
Stain used for wbc counts, cell morphology and ID of blood borne parasites
Romanowsky
- diff-quik
- leishman’s
- giesma
Stain used for reticulocyte counts and detecting heinz bodies
Methylene blue
