Blood Components (Plasma Products and Derivatives)

Question 1

Plasma from the whole blood donation can be
manufactured into a variety of components

Answer 1

Fresh Frozen Plasma

Question 2

Component variety of FFP: (2)

Answer 2

1. Frozen
2. Cryoprecipitate

Question 3

How is FFP prepared:

Answer 3

prepared by removing plasma from a whole blood and frozen within 8 hours of collection

Question 4

Indication of FFP: (6)

Answer 4

1. Transfused to justify for the replacement of plasma protein when specific concentrates are not available
2. Treatment of multiple coagulation factor deficiencies.
3. Massive Transfusion
4. Trauma
5. Liver Disease
6. DIC

Question 5

Multiple coagulation factor
deficiencies that FFP is used for: (6)

Answer 5

1. Bleeding
2. Unavailability of Factor VIII and VIII concentrate
3. Abnormal Coagulation Assay
4. Anti-thrombin pre -treatment
5. Thrombotic Thrombocytopenic Purpura
6. Hemolytic Uremic Syndrome

Question 6

Contraindication of FFP:

Answer 6

Not appropriate as volume expander

Question 7

Shelf life of FFP:

Frozen:
Thawed:

Answer 7

Frozen: 1 year or 7 years

Thawed: 37°C water bath for 24 hours

Question 8

Storage temp of FFP:

Frozen:
Thawed:

Answer 8

Frozen: -18°C or colder

Thawed: 30-37°C water bath followed by 1-6°C storage after thawing.

Question 9

How long must thawed FFP be transfused before it no longer becomes viable?

Answer 9

24 hours

Question 10

Contents of FFP

Answer 10

All coagulation factors including 400 mg of fibrinogen

Question 11

Its indication is for treatment of stable clotting factor deficiencies

Answer 11

Single Donor Plasma

Question 12

Shelf Life of Single Donor Plasma:

Liquid:
Frozen:

Answer 12

Liquid: 5 days beyond whole blood expiration
Frozen: 5 years

Question 13

Storage Temp of Single Donor Plasma:

Liquid:
Frozen:

Answer 13

Liquid: 1-6°C
Frozen: -18°C or colder

Question 14

Plasma component that is prepared from Fresh Frozen Plasma that is thawed at 30-37°C and stored at 1-6°C

Answer 14

CRYOPRECIPITATED ANTIHEMOPHILIC FACTOR

Question 15

Indications of CRYOPRECIPITATED ANTIHEMOPHILIC FACTOR:

Answer 15

1. Hemophilia A
2. von Willebrand’s disease
3. Fibrinogen deficiency
4. Factor XIII deficiency (Stable clotting factor)

Question 16

Shelf life of Single Donor Plasma:

Frozen:
Thawed:
Pooled:

Answer 16

Frozen: 1 year
Thawed: 6 hours
Pooled: 4 hours

Question 17

Storage Temp of Single Donor Plasma

Frozen:
Thawed:

Answer 17

Frozen: -18°C or colder
Thawed: Room temp (20-24°C)

Question 18

Contents of Single Donor Plasma and its percentage: (4)

Answer 18

Factor VIII:C - 80-150 IU
Factor VIII:vWF – 40-70%
Fibrinogen – 150-250 mg
Factor XIII – 20-30%

Question 19

Limited to patients that undergo chemotherapy that is prone to neutropenia

Answer 19

GRANULOCYTE CONCENTRATE

Question 20

How is granulocyte concentrate prepared?

Answer 20

Prepared via apheresis as it provides high yield product compared when it is prepared via whole blood.

Question 21

Therapeutic dose of granulocyte concentrate:

Answer 21

1.0 x1010 per day

Question 22

Indications of granulocyte concentrate:

Answer 22

To correct severe neutropenia

Fever unresponsive to antibiotic/antifungal therapy

Myeloid hypoplasia of the bone marrow

Question 23

Shelf life of granulocyte concentrate:

Answer 23

24 hours

Question 24

Storage temp of granulocyte concentrate:

Answer 24

20-24°C without the need of agitation

Question 25

Contents of granulocyte concentrate:

Answer 25

≥ 1.0 x 1010 WBC

Question 26

Concentrates of plasma proteins that are prepared from pools of plasma

Answer 26

Plasma Derivatives

Question 27

Given to patients with hemophilia A in relation to FVIII deficiency

Answer 27

FACTOR VIII CONCENTRATE

Question 28

Shelf life of factors VIII and IX concentrate:

Answer 28

Varies on the expiration date on the vial

Question 29

Storage temp of factors VIII and IX

Answer 29

1-6°C (Lyophilized)

Question 30

Indication of: Factor VIII: Factor IX:

Answer 30

Factor VIII: Hemophilia A Factor IX: Hemophilia B

Question 31

Primarily IgG antibody. Used for patients with Congenital Hypogammaglobulinemia

Answer 31

Immune Serum Globulin

Question 32

Shelf life of Immune Serum Globulin

Answer 32

Depend on the route of administration o Intramuscular: 3 years o Intravenous: 1 year

Question 33

Indication of Immune Serum Globulin:

Answer 33

1. Prophylactic treatment for those exposed to hepatitis, measles, and chicken pox. 2. Hypogammaglobulinemia

Question 34

Given to those requiring plasma volume expansion

Answer 34

Normal Serum Albumin

Question 35

Shelf life and storage of normal serum albumin

Answer 35

3 years at 20-24°C 5 years at 1-6°C

Question 36

Content of Normal Serum Albumin:

Answer 36

96% Albumin 4% Globulin

Question 37

Prepared the same as how NSA is prepared, however, it does not have as much as high albumin content.

Answer 37

Plasma Protein Fraction

Question 38

Indication of PPF:

Answer 38

Plasma Volume Expansion

Question 39

Shelf life and storage of Plasma Protein Fraction:

Answer 39

3 years at 20-24°C 5 years at 1-6°C

Question 40

Contents of Plasma Protein Fraction:

Answer 40

85% Albumin 15-20% Globulin

Question 41

Required Dose of Irradiation of Irradiated Blood:

Answer 41

o 25(gy) in the middle of the canister o 15 (gy) – lowest or minimum dose

Question 42

Importance of Irradiating Blood

Answer 42

Indicated by AABB Standards, it is important in irradiating blood components if: o The donor unit is from a blood relative of the recipient o The donor unit’s HLA match the patient  Note: T-lymphocyte in the blood can cause TA-GVDH

Question 43

Given to females that are Rh (-)ve that are pregnant with Rh (+)ve child

Answer 43

RhoGAM

Question 44

Shelf life and storage of RhoGAM

Answer 44

3 years at 1-6°C

Question 45

Content of RhoGam: Full Dose Mini Dose

Answer 45

Full dose: 300ug Anti-D Mini dose: 50ug Anti-D

Question 46

Indication of RhoGAM

Answer 46

Prevention of Rho (D) immunization

Question 47

Provide a safe alternative with regards to disease transmission because they are already sterilized and contain no human plasma

Answer 47

Synthetic Volume Expanders

Question 48

Examples of Synthetic Volume Expanders: (5)

Answer 48

NSS Electrolyte Solution Hydroxyethyl starch (HES) Ringer’s lactate Dextran

Question 49

Crystalloid vs. Colloids Crystalloid Best Example: IV Retention: Peripheral Edema: Pulmonary Edema Excretion: Allergic Reactions: Cost

Answer 49

Best Example: Ringer’s lactate IV Retention: Poor Peripheral Edema: Common Pulmonary Edema: Possible Excretion: Easily excreted Allergic Reactions: Absent Cost: Cheaper/inexpensive

Question 50

Crystalloid vs. Colloids Colloid Best Example: IV Retention: Peripheral Edema: Pulmonary Edema Excretion: Allergic Reactions: Cost

Answer 50

Best Example: Dextran IV Retention: Good Peripheral Edema: Possible Pulmonary Edema: Possible Excretion: Not easily excreted Allergic Reactions: Rare Cost: Expensive