Blood Component Preparation and Transfusion Therapy Flashcards
Blood collected from a donor before separation into its components
Whole Blood
Parts of whole blood that can be separated by centrifugation consists of
RBC
Plasma
Cryoprecipitated Antihemophilic Factor
Platelets
Treatment of a disease or condition by the use of blood or blood derivatives
Hemotheraphy
Collection of blood in a sterile blood container
Closed System
Collection or exposure to air through an open port that would shorten the expiration because of potential bacterial contamination
Open System
What solution works to prevent clotting and extend the sstorage of Red Blood Cells
Anticoagulant Preservative Solution
The volume of anticoagulant preservative solution in the primary collection bag is ___mL or _____ mL
63 mL or 70 mL
The standard whole blood collection volume is _____ mL for blood collected in a bag containing ____ mL of anticoagulant or ______ mL for the larger volume bag containing _____ mL of anticoagulant preservative
450+-45
63mL
500+_50
70 mL
Reducing the anticoagulant may be necessary if a unit of blood is collected from an individual weighing less than ____lb
110 lb
Heavy Spin for PRBC, Platelet concentrate
5000 g for 5 mintues
Heavy Spin for Cryo, Cell free plasma
5000 G FOR 7 minutes
Heavy Spin for platelet - rich plasma
2000 g for 3 minutes
Binds calcium, prevents coagulation by chelating calcium, alsoprotects red cell membrane
Citrate
Provides energy to RBC, supports ATP Generation by glycolytic pathway
Dextrose
To lower the pH, Prevents caramelization
Citric Acid
Increase ATP, prevents excessive decrease in pH
Phosphate Buffer
Improves survival of RBC, Acts as substrate for red cell ATP synthesis
Adenine
In Mannitol, ____ acts as membrane stabilizer
Osmotic Diuretic
Days forCPD
21
Days for CP2D
21
Days for CPDA 1
35
Days for AS-1 (Adsol)
42
Days for AS-3 (Optisol)
42
It is used in some blood centers to regenerate ATP and 2,3 DGP
Rejuvination Solutions
In rejuvenation solution, red cells stored in the liquid state for fewer than ___ days after their outdate are rejuvanted for ___ to ___ hours, at ___ deg C with the solution
3 DAYS
1 - 4HOURS
37 Deg C
What is PIPA in rejuvination solution
Phosphate, Inosine, Pyruvate, Adenine
Only FDA approved rejuvenation solution in US
Rejuvesol
What is PIGPA in rejuvination solution
Phosphate, Inosine, Gluose, Pyruvate, Adenine
Whole blood is the unmodified component drawn from a donor, which consists of ___, ___, ___, and ____ with anticoagulant preservative solution
Erythrocyte, Leukocyte, and Plasma Proteins
Wholeb lood is stored in a monitored refrigerator at ___ - ____ Deg C for ___ days if collected in CPD or for ___ days if collected in CPDA 1
1-6 Deg C
21 days
35 days
Storage for WHOLE BLOOD
1-6C
Transport for WHOLE BLOOD
1-10 C
Heparin for WHOLE BLOOD
2 days
RBC contains ___, which transports oxygen from tissues and the bloodstream
Hemoglobin
Transfusing __ unit of RBCs usually increases the hemoglobin by ___ g/dL and increases the hematocrit by ___ %
1 unit
1 g/dL
35 %
Storage of RBC
1-6 Deg C
Shelf life of RBC
Closed (Depends on anticoagulant)
Open (24 Hrs)
Is designed to remove leukocyte from whole blood without removing platelets
Inline Filters
In frozen red blood cells, freezing extends storage up to ___ years from collection when stored at or below ___ deg C
10 Years
-65 deg C
Freezing can also be accomplished with a lower glycerol concentration if a _____ is used
Liquid Nitrogen Freezer
Glycerol concentration is approximately ___% and the initial freezing temperature is ___ Deg C
20%
-196 Deg C
In frozen red blood cells, the maximum temperature of freezing is ____ Deg C for ____ Years
-120 Deg C
10 Years
What is the process of thawing, and removing the glycerol from Frozen RBCs
Deglycerolization
The process of glycerolization and deglycerolization involves entering of blood unit, the system is considered ____ and the prduct must be transfused within ____ days if closed system
Open System
14 Days
In Washed Red Blood Cells, the reaction can be ____, ____, or ____.
Allergic
Febrile
Anaphylactic
Washing is accomplished with approximately _____ mL of ____% Saline using the ________
1000 mL
0.9% SALINE
Automatic blood cell processor
In Red Blood Cells Irradiated, Irradiation can be performed with a
Gamma Radiator
Linear Accelerators
Ultraviolet A Irradiation
Nonradioisotopes equipment (xray)
The required dose of irradiation is _____ cGy or ____ Gy, the lowest dose should be _____ cGy
2500 cGy or 25 Gy
1500 cGy
The expiration date of irradiated RBCs is changed to ____ days after irradiation if the available shelf life exceeds ___ days
28 days
28 days
Transfused platelets normally circulate with a lifespan of ____ days
3-4 days
Platelet concentrates should contain at least _____ platelets per unit, and under optimal conditions, it should elevate the platelet count by about ____%L in a recipient weighing ____kg
5.5x1010
5000%L
75 kg
In pooled platelets, the approximate dose is ____ unit per ___ of body weight
1 unit per 10/kg of patient body weight
The pooled platelets should be stored at ____ to ____ deg C
20-24 deg C
It is an effective method of harvesting therapeutic dose of platelets from one individual donor
Apheresis Platelets
Plateletpheresis donors may donate as often as ____ a week or ____ ayear with an interval of ____ hours between procedures
2x a week
24 times a year
48 hours
A unit of platelets prepared by apheresis should contain a minimum of _____ platelets in _____% of the samples units
3x1011 platelets
90%
FFP (Fresh Frozen Plasma) contains all coagulation factor including ____, _____ which do not store well at temperatures greater than _____ deg C
Labile factors V
VIII
-18 deg C
Units of FFP and PF24 are thawed before administration in a ______ to _____ deg C waterbath for approximately _____ to ____ minutes
30-37 deg C
30-45 minutes
Standard microwave ovens should never be used because they denature ______
Plasma Proteins
FFP (Fresh Frozen Plasma) is stored at ____ to ____ deg C and should be transfused within ____ hours from thawing
1-6 deg C
24 hours
In FFP, if not transfused within 24 hours, the product should be relabeled as ______ and stored at ____ to ____ deg C for up to ____ days after thawing
“Thawed Plasma”
1-6 deg C
5 days
Storage for FFP
-18 deg C or colder
Shelf life for FFP
1 year/12 months
What is the cold insoluble precipitate that forms when a unit of FFP is thawed between 1 deg c and 6 deg C
Cryoprecipitated AHF (ANTIHEMOPHILIC FACTOR)
Factors in cryoprecipitated AHF
Von Willebrand Factor
Fibrinogen (Factor 1)
Factor VII
Fibronectin
Factor X11
Storage for Cryoprecipitated AHF
-18 deg C or colder
Shelf life for Cryoprecipitated AHF
1 year / 12 months
The primary clinical uses of CRYO are as a supplement for patients with deficiencies of ____ and _____
Factor XIII and Fibrinogen (Factor I)
In Plasma Cryoprecipitated Reduced, the product is relabeled as ______ and is stored at ____ deg C or lower for ___ year from the date of collection
Plasma Cryoprecipitated Reduced
-18deg C
1 year
The CRYO Poor plasma (CPP) is used primarily in the treatment of ________ because it contains ______
Thrombotic Thrombocytopenic Purpura (TTP)
ADAMTS 13
In cryoprecipitated antihemophilic factor (pooled), the frozen units first must be thawed in a ____ to ____ water bath for up to ____ minutes using ____ to prevent contamination of the ports
30-37 deg C
15 minutes
over wraps
In apheresis granulocytes, the number of granulocytes in each product equals or is greater than ______
1.0x1010
Granulocytes deteriorate rapidly on storage and should be administered within ___ hours of collection.
24 hours
Storage for Apheresis Granulocyte
20-24 deg C without agitation
Shelf life for Apheresis Granulocyte
24 Hours
Shelf life for Modified Whole Blood
21 days at 1-6 deg C
Transfused platelets normally circulatewith a lifespan of how many days?
3-4 days
PLATELETS LEUOKOCYTES REDUCED
Platelets collected by apheresis must have less than ____ leukocytes in ____% of the units tested
5 x 106
95%
The primary clinical use of CRYO are as a supplement for patients with deficiencies of factor ___ and ___
Factor XII
Factor I
What is the protein that is reduced in Thrombotic Thrombocytopenic Purpura?
ADAMTS 13
Pooled CEYO must be administered within ___ hours of first entry and should be stored at ___ until transfusion?
4 hours
Room Temperature
STORAGE for Apheresis Granulocyte
20-24 C with agitation
STORAGE for fresh frozen plasma
-18 or -65C
STORAGE for Thawed Plasma
1-6 C
Storage for Apheresis FFP
-18C
STORAGE for cryoprecipitated AHF (After Thawing)
20-24 C
For cancer patients(chemotheraphy) and recipients of hematopoietic progenitor transplants
Platelets
