Blood collection tubes Flashcards
What does LTT or PTT stand for ?
Lavendar Top Tube or Purple Top Tube
LTT or PTT contains the _________ _______ which binds with calcium to prevent coagulation.
anticoagulant EDTA
LTT or PTT is indicated for use with _____ studies. What else is ran on the plasma?
CBC
BUN - blood, urea, nitrogen
LTT or PTT fluid portion if spun is called?
plasma
What are the advantages of LTT or PTT?
causes least amount of cell distortion/destruction, best preservation of cells, fairly stable for 4-6 hours
What are disadvantages of LTT orPTT?
after 6 hours - WBC begin to break down and disintegrate, after 4 hours - platelets swell and clump, incorrect blood to EDTA ration causes cells to shrink
What is EDTA?
Ethylenediaminetetraacetic acid
What are special considerations with LTT or PTT?
Refrigerate blood if. not able to run tests immediately. This will minimize damage to cells.
Rotate tube gently and thoroughly to prevent clotting and hemolysis
What does GTT stand for?
green tube top
GTT contains the _____ ______ wihich prevents conversion of prothrombin to thrombin.
anticoagulant heparin
GTT is indicated for use in ______ _____ determinations. i.e. _____ and ______ levels
blood gas
ie. CO2 and O2
With GTT, if tube also contains Lithium, it will be used for?
blood chemistries at AEVH and clinics that use the VET Test blood chemistry machine.
With GTT, if the fluid portion is spun, it is called?
plasma
What are the advantages in GTT’s?
maintains size and shape of RBC even in poor blood to anticoagulant ratio, best from blood gas reading and hormone and drug levels in blood, used for reptile hematologic studies
What are the disadvantages in GTT’s?
causes hazy staining with routine type differential stains, WBC may clump, expensive
What does BTT stand for?
Blue Top Tube
What does BTT contain?
contains the anticoagulant sodium citrate
In BTT what does sodium citrate bind with?
calcium to prevent coagulation
BTT are indicated for?
coagulation studies
In BTT’s the fluid portion if spun is called?
citrated plasma
What are the advantages of BTT’s?
used for most coagulation studies needed anticoagulated blood, non-toxic and can be used for blood transfusions
What are the disadvantages of BTT’s?
shrinks cells, tube must be filled, ration of blood to anticoagulant is crucial
What should you never do with BTT’s?
never remove top - let vacuum do its job
What does GTT stand for?
grey top tube
GTT’s contain the anticoagulant ______ (NH4 or K) which binds with _____ to prevent coagulation.
Oxylate
calcium
GTT’s are indicated for use in ______ ______ level determinations
blood glucose
GTT’s fluid portion is called?
plasma
What are the advantages of GTT’s?
maintains cell wall integrity eliminating movement of glucose from plasma into the cell
What are the disadvantages of GTT’s?
- K oxlate shrinks cells
- NH4 oxylate swells cells
- Cannot be used for BUN determinatins due to NH4
- oxylate crystals seen on differential so not able to use for CBC
What does RTT stand for?
Red top tube
RTT contains no _______ so blood will clot.
anticoagulant
RTT separates out in centrifuges into ______ portion and ______ portion. Fluid portion is called?
cellular
fluid
serum
RTT indicated for use in ______ _______ studies.
blood chemistry
Plan RTT very poor for ______ _______ studies, should also draw a _____ with it.
blood glucose
GTT
RTT can be purchased with a gel like substance that when spun will _______ the cells and _______.
separate
serum
RTT can be used for _____ ______ studies, helps decrease usage of glucose by the cells.
blood glucose
RTT is called a ______ separator tube
serum
TT stands for?
Tiger tube
