Blood collection tubes Flashcards

1
Q

What does LTT or PTT stand for ?

A

Lavendar Top Tube or Purple Top Tube

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2
Q

LTT or PTT contains the _________ _______ which binds with calcium to prevent coagulation.

A

anticoagulant EDTA

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3
Q

LTT or PTT is indicated for use with _____ studies. What else is ran on the plasma?

A

CBC

BUN - blood, urea, nitrogen

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4
Q

LTT or PTT fluid portion if spun is called?

A

plasma

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5
Q

What are the advantages of LTT or PTT?

A

causes least amount of cell distortion/destruction, best preservation of cells, fairly stable for 4-6 hours

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6
Q

What are disadvantages of LTT orPTT?

A

after 6 hours - WBC begin to break down and disintegrate, after 4 hours - platelets swell and clump, incorrect blood to EDTA ration causes cells to shrink

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7
Q

What is EDTA?

A

Ethylenediaminetetraacetic acid

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8
Q

What are special considerations with LTT or PTT?

A

Refrigerate blood if. not able to run tests immediately. This will minimize damage to cells.
Rotate tube gently and thoroughly to prevent clotting and hemolysis

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9
Q

What does GTT stand for?

A

green tube top

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10
Q

GTT contains the _____ ______ wihich prevents conversion of prothrombin to thrombin.

A

anticoagulant heparin

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11
Q

GTT is indicated for use in ______ _____ determinations. i.e. _____ and ______ levels

A

blood gas

ie. CO2 and O2

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12
Q

With GTT, if tube also contains Lithium, it will be used for?

A

blood chemistries at AEVH and clinics that use the VET Test blood chemistry machine.

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13
Q

With GTT, if the fluid portion is spun, it is called?

A

plasma

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14
Q

What are the advantages in GTT’s?

A

maintains size and shape of RBC even in poor blood to anticoagulant ratio, best from blood gas reading and hormone and drug levels in blood, used for reptile hematologic studies

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15
Q

What are the disadvantages in GTT’s?

A

causes hazy staining with routine type differential stains, WBC may clump, expensive

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16
Q

What does BTT stand for?

A

Blue Top Tube

17
Q

What does BTT contain?

A

contains the anticoagulant sodium citrate

18
Q

In BTT what does sodium citrate bind with?

A

calcium to prevent coagulation

19
Q

BTT are indicated for?

A

coagulation studies

20
Q

In BTT’s the fluid portion if spun is called?

A

citrated plasma

21
Q

What are the advantages of BTT’s?

A

used for most coagulation studies needed anticoagulated blood, non-toxic and can be used for blood transfusions

22
Q

What are the disadvantages of BTT’s?

A

shrinks cells, tube must be filled, ration of blood to anticoagulant is crucial

23
Q

What should you never do with BTT’s?

A

never remove top - let vacuum do its job

24
Q

What does GTT stand for?

A

grey top tube

25
Q

GTT’s contain the anticoagulant ______ (NH4 or K) which binds with _____ to prevent coagulation.

A

Oxylate

calcium

26
Q

GTT’s are indicated for use in ______ ______ level determinations

A

blood glucose

27
Q

GTT’s fluid portion is called?

A

plasma

28
Q

What are the advantages of GTT’s?

A

maintains cell wall integrity eliminating movement of glucose from plasma into the cell

29
Q

What are the disadvantages of GTT’s?

A
  1. K oxlate shrinks cells
  2. NH4 oxylate swells cells
  3. Cannot be used for BUN determinatins due to NH4
  4. oxylate crystals seen on differential so not able to use for CBC
30
Q

What does RTT stand for?

A

Red top tube

31
Q

RTT contains no _______ so blood will clot.

A

anticoagulant

32
Q

RTT separates out in centrifuges into ______ portion and ______ portion. Fluid portion is called?

A

cellular
fluid

serum

33
Q

RTT indicated for use in ______ _______ studies.

A

blood chemistry

34
Q

Plan RTT very poor for ______ _______ studies, should also draw a _____ with it.

A

blood glucose

GTT

35
Q

RTT can be purchased with a gel like substance that when spun will _______ the cells and _______.

A

separate

serum

36
Q

RTT can be used for _____ ______ studies, helps decrease usage of glucose by the cells.

A

blood glucose

37
Q

RTT is called a ______ separator tube

A

serum

38
Q

TT stands for?

A

Tiger tube