Blood collection tubes Flashcards
What does LTT or PTT stand for ?
Lavendar Top Tube or Purple Top Tube
LTT or PTT contains the _________ _______ which binds with calcium to prevent coagulation.
anticoagulant EDTA
LTT or PTT is indicated for use with _____ studies. What else is ran on the plasma?
CBC
BUN - blood, urea, nitrogen
LTT or PTT fluid portion if spun is called?
plasma
What are the advantages of LTT or PTT?
causes least amount of cell distortion/destruction, best preservation of cells, fairly stable for 4-6 hours
What are disadvantages of LTT orPTT?
after 6 hours - WBC begin to break down and disintegrate, after 4 hours - platelets swell and clump, incorrect blood to EDTA ration causes cells to shrink
What is EDTA?
Ethylenediaminetetraacetic acid
What are special considerations with LTT or PTT?
Refrigerate blood if. not able to run tests immediately. This will minimize damage to cells.
Rotate tube gently and thoroughly to prevent clotting and hemolysis
What does GTT stand for?
green tube top
GTT contains the _____ ______ wihich prevents conversion of prothrombin to thrombin.
anticoagulant heparin
GTT is indicated for use in ______ _____ determinations. i.e. _____ and ______ levels
blood gas
ie. CO2 and O2
With GTT, if tube also contains Lithium, it will be used for?
blood chemistries at AEVH and clinics that use the VET Test blood chemistry machine.
With GTT, if the fluid portion is spun, it is called?
plasma
What are the advantages in GTT’s?
maintains size and shape of RBC even in poor blood to anticoagulant ratio, best from blood gas reading and hormone and drug levels in blood, used for reptile hematologic studies
What are the disadvantages in GTT’s?
causes hazy staining with routine type differential stains, WBC may clump, expensive