Blood Collection Equipment Flashcards
Chapter 8
What equipment do you need for a venipuncture?
- Needles
- Tube holder
- Tourniquet
- Antiseptics (alcohol, chlrohexidine gluconate)
- Vacuum collection tubes
- Labels
- Gloves
- Requisition
- Patient
- Band aid / gauze
- Hand sanitizer
- Black sharpie
- Biohazard bag
- Gauze
What antiseptic will you use if a patient is allergic to alcohol?
Chlorhexidine gluconate
Tube order of draw
Yellow (SPS)
Light blue
Red
Gold
Light Green
Green
Lavender
Pink
Tan
Royal Blue
Gray
Yellow ADC
What does SST stand for?
Serum Separator Tube
What color is the SST tube?
Gold
What does SPS stand for?
Sodium polyanethol sulfonate
What color is the SPS tube?
Yellow
What color is the ACD tube?
Yellow
FUO
Fever with unknown origin
What tube is used to determine the cause of an FUO?
Yellow SPS / Culture vial
What is EDTA?
An additive that causes the blood to remain fluid for years.
Define phagocytosis
when a cell eats another cell. antibiotics cause white blood cells to eat bacteria or infested cells.
Define antiseptics
chemicals used to inhibit the growth and development of microorganisms, but this doesn’t kill them
Define disinfectant
chemical compounds used to remove or kill pathogenic microorganisms
essential antiseptics for blood collection
70% isopropyl alcohol | iodine swab | chlorhexidine gluconate
How long does it take antiseptics to dry?
30-60 seconds
Why do we use disinfectants before a venipuncture?
Used to prevent contamination on the skin
Anticoagulant
additive that prevents clotting
ex. EDTA
Clot activators
additives that promote clotting
ex.
An anticoagulant medication
aspirin
A CBC test requires whole blood collected in what color tube
lavender
This color tube is used to test for Lead
tan
This tube is known as serum separation tube (SST)
gold
This tube has the additive EDTA in it
lavender
This tube is used for STAT testing
orange
This tube is used to test for nutrient determination
dark blue
This needle is used for hand draws
butterfly needle
How many times should a lavender top be inverted
8-10 times
This tube colors is used to test for PT/INR
light blue
This color tube is used to test for paternity
yellow acd
The color coding for needles indicates the
gauge size
This color tube is used to test a person’s Blood Type
pink
The amount of time a tourniquet can be applied on the arm
60 seconds
This tube has no additives in it
red
These tubes have the additive heparin in it
green
This tube is used to test glucose tolerance
gray
This part of the needle always needs to be up
bevel
The hole of a needle is also known as the
lumen
This holds the tube while it fills with blood
tube holder
The device blood flows into as it’s being collected
vacuum holder
Which tube is used to test for Septicemia or FUO
yellow SPS
This color tube is used to test for Hemoglobin A1C
lavender
This is the liquid portion of blood
plasma
Inversion times for the light blue tube
4 - 5
How is serum collected?
centrifuge a serum tube > suction out serum from the top > transfer serum into another container for transport to the lab
Anticoagulant tubes
tubes where the blood remains fluid
- light blue
- lavender
- green (heparin)
- gray
- SPS
Clot activator tubes
tubes where the blood clots and becomes solid
- red
- SST
- orange (RST)
- gold
- marble
Whole blood
blood collected in anticoagulant tubes and only requires inversion
Plasma
liquid portion of the blood
- collected in anticoagulant tubes and centrifuged
Serum
collected in coagulant tubes and separated from blood cells via centrifuge
Whole blood tubes
- yellow, SPS
- lavender
- yellow, ACD
- pink
Plasma tubes
- light blue
- gray
- green
- light green
- royal blue
- tan
- pink
Serum tubes
- red (60 mins)
- gold (30 mins)
- tiger top (30 mins)
- gray
- light green
- orange (5 mins)
- royal blue
Largest gauge of a needle
22
Typical needle gauge to use on adults
21G
the smaller the gauge,
the larger the needle
Needles color code by
gauge size
Hemolysis
rupture or lysis of blood cells, which can be cause by using a needle that is too small
Gauge used for blood donations
18 G
Tests done on Light blue tube
- coagulation tests
Tests done on Red tubes
- therapeutic drug monitoring
Tests done on Gold tubes
-HCG
- Lytes
- LFTS
- Iron
- Hormones
- Any panels
Tests done on Green tubes
- Lactate
- Ammonia
- Plasma K
Tests done on Lavender tubes
- Blood count (CBC)
- Hemaglobin A1C
- Sickle cell
- Platelet count
- Bone marrow
- Retics
- ESR
- CD4
Tests done on Pearlescent tubes
You don’t, it’s a discard tube
Tests done on Pink tubes
- Cross match blood donations
- Transfusion medicine
Tests done on Yellow SPS tubes
- blood cultures
- FUO
Tests done on Orange tubes
- STAT serum determinations
Tests done on Royal blue tubes
- nutritional tracing
- toxicology tests (overdosing)
- Lead
- Zinc
- Chromium
- Cobalt
Tests done on Gray tubes
- pregnancy glucose
- glucose testing
- ETOH (alcohol)
- Lactose testing
Tests done on Yellow ACD
- paternity testing
- organ donation compatibility
Which anticoagulant is found in the white top tube?
EDTA
For capillary collection from newborns, a lancet of which lengths should be used to avoid penetrating the bone?
anything less than 2.0 mm
If a heparin tube is underfilled, which of the following analytes performed with blood from a heparin tube will have false values?
Troponin
What is an anticoagulant used in blood donations?
ACD
The glycolytic inhibitor is found in which tube
gray
Which tube cannot be used to collect for the CPK, Troponin T, and other cardiac assays since the additive would destroy the analytes?
gray
Which tube should be used for collection of blood for the complete blood count?
lavender
Which tube should be used for collecting the blood to go to the blood bank for the type and cross-match?
pink
D-Dimer
Light blue
Panels
SST
Bilirubin
SST
BUN
SST
Calcium
SST
Cholesterol
SST
Creatinine
SST
Ferritin
SST
Follicle stimulating hormone
SST
Glucose, Fasting
SST
HCG
SST
HIV
SST
H-Pylori
SST
Lyme Disease
SST
Magnesium
SST
Potassium
SST
Prostate Specific Antigen
SST
Sodium
SST
Testosterone
SST
Vitamin B12
SST
Thyroid Stimulating Hormone
SST
Antibody identification
Red
Antibody screening
Red
Digoxin
Red
Lithium
Red
Serum tests
Red
Discard tube
Red
Blood bank type
Lavender
Bone marrow
Lavender
Cerebrospinal fluid
Lavender
Complete blood count (CBC)
Lavender
Platelets
Lavender
Hematocrit
Lavender
Hemoglobin
Lavender
Erythrocyte sedimentation rate
Lavender
Hemoglobin A1C
Lavender
White blood cell
Lavender
Sickle cell
Lavender
Red blood cells
Lavender
Glucose tolerance
Gray
Lactose tolerance
Gray
Pregnancy glucose testing
Gray