Blood Banking Techniques Flashcards
Using antisera to detect antigens on patient’s RBCs
Forward grouping/direct typing/front typing/cell typing
Preservative used in forward grouping
Sodium azide/formalin
Amount of typing sera used in forward grouping
2 drops
Color and name of anti-A sera
Blue (trypan blue)
Color and name of anti-B sera
Yellow (acriflavine yellow)
Amount of specimen used in forward grouping
1 drop RCS
Expected reaction in forward grouping
3+ to 4+
Using reagent RBCs to detect ABO antibodies in the patient’s serum
Reverse grouping/indirect typing/back typing/serum typing
Amount of reagent used in reverse grouping
1 drop
Amount of specimen used in reverse grouping
2 drops patient serum
Expected reaction in reverse grouping
2+ - 4+
Universal RBC donor
O
Universal plasma donor
AB
Universal RBC recipient
AB
Universal plasma recipient
O
One solid agglutinate
4+
Several large agglutinates, clear background
3+
Medium agglutinates, clear background
2+
Small agglutinates, turbid background
1+
Tiny agglutinates, turbid background
W+
No agglutination or hemolysis
0
Partial or complete hemolysis is a ______ reaction
+
Hemolysis-when there is presence of free Hb in the serum
H; 10
One solid aggutinate
4+; 10
