Blood Banking Techniques

Question 1

Using antisera to detect antigens on patient’s RBCs

Answer 1

Forward grouping/direct typing/front typing/cell typing

Question 2

Preservative used in forward grouping

Answer 2

Sodium azide/formalin

Question 3

Amount of typing sera used in forward grouping

Answer 3

2 drops

Question 4

Color and name of anti-A sera

Answer 4

Blue (trypan blue)

Question 5

Color and name of anti-B sera

Answer 5

Yellow (acriflavine yellow)

Question 6

Amount of specimen used in forward grouping

Answer 6

1 drop RCS

Question 7

Expected reaction in forward grouping

Answer 7

3+ to 4+

Question 8

Using reagent RBCs to detect ABO antibodies in the patient’s serum

Answer 8

Reverse grouping/indirect typing/back typing/serum typing

Question 9

Amount of reagent used in reverse grouping

Answer 9

1 drop

Question 10

Amount of specimen used in reverse grouping

Answer 10

2 drops patient serum

Question 11

Expected reaction in reverse grouping

Answer 11

2+ - 4+

Question 12

Universal RBC donor

Answer 12

O

Question 13

Universal plasma donor

Answer 13

AB

Question 14

Universal RBC recipient

Answer 14

AB

Question 15

Universal plasma recipient

Answer 15

O

Question 16

One solid agglutinate

Answer 16

4+

Question 17

Several large agglutinates, clear background

Answer 17

3+

Question 18

Medium agglutinates, clear background

Answer 18

2+

Question 19

Small agglutinates, turbid background

Answer 19

1+

Question 20

Tiny agglutinates, turbid background

Answer 20

W+

Question 21

No agglutination or hemolysis

Answer 21

0

Question 22

Partial or complete hemolysis is a ______ reaction

Answer 22

+

Question 23

Hemolysis-when there is presence of free Hb in the serum

Answer 23

H; 10

Question 24

One solid aggutinate

Answer 24

4+; 10

Question 25

Several medium to large agglutinates

Answer 25

3+; 8

Question 26

Many small to medium agglutinates; clear background

Answer 26

2+; 5

Question 27

Many small agglutinates, turbid background

Answer 27

1+; 3

Question 28

Few small agglutinates with many unagglutinated cells

Answer 28

+/w

Question 29

Aggregates visible only under a microscope

Answer 29

+-“’/+”’; 1

Question 30

No agglutination or hemolysis

Answer 30

0; 0

Question 31

Presence of minor population of agglutinated cells superimposed on a negative background

Answer 31

MF

Question 32

Nonspecific aggregation like a stack of coins

Answer 32

Rouleaux