Blood Bank Lab Final Flashcards
What temperature are RBCs stored at in the blood bank?
1-6 C
What temperature is thawed FFP stored at in the blood bank?
1-6 C
What temperature are platelets stored at in the blood bank?
20-24 C (RT)
What temperature is thawed cryo stored at in the blood bank?
20-24 C (RT) because it must be used within 6 hours of thawing
What temperature is frozen FFP stored at in the blood bank?
-18 C
What temperature is frozen tissue stored at in the blood bank?
-80 C
What is the expiration on AS-5 pRBC?
42 days
When do platelets expire?
5 days from collection
When does thawed FFP exire?
5 days exactly
Candidates for cord blood testing
- Rh negative mom
- Mom with significant antibodies
- Mom that is type O
- Mom with no prenatal history
- NICU baby
Candidates for Rhogam
When do we do weak D testing (what group of people)
Neonates and blood donors
Compare/contrast IAT and DAT
How are antibodies are acquired/formed and examples?
Naturally occurring (ABO)
Passively (through mom)
Immune (infection creates memory)
What cells will be your positive control for antigen typing?
Heterozygous cells (weakest reaction)
What select cells are needed to rule out an antibody?
Cells that are positive for the antibody you want to rule out and negative for the antibody you want to rule in
Forward type: A
Back type: AB
What is the discrepancy and how would you resolve it?
Weak/missing reaction - incubate plasma and reagents at RT for 15 minutes
Miller Run steps
Frequency of K antigen in the population
About 10%
Post-transfusion tests
ABORh and DAT poly
