Blood Bank Lab Final

Question 1

What temperature are RBCs stored at in the blood bank?

Answer 1

1-6 C

Question 2

What temperature is thawed FFP stored at in the blood bank?

Answer 2

1-6 C

Question 3

What temperature are platelets stored at in the blood bank?

Answer 3

20-24 C (RT)

Question 4

What temperature is thawed cryo stored at in the blood bank?

Answer 4

20-24 C (RT) because it must be used within 6 hours of thawing

Question 5

What temperature is frozen FFP stored at in the blood bank?

Answer 5

-18 C

Question 6

What temperature is frozen tissue stored at in the blood bank?

Answer 6

-80 C

Question 7

What is the expiration on AS-5 pRBC?

Answer 7

42 days

Question 8

When do platelets expire?

Answer 8

5 days from collection

Question 9

When does thawed FFP exire?

Answer 9

5 days exactly

Question 10

Candidates for cord blood testing

Answer 10

1. Rh negative mom
2. Mom with significant antibodies
3. Mom that is type O
4. Mom with no prenatal history
5. NICU baby

Question 11

Candidates for Rhogam

Question 12

When do we do weak D testing (what group of people)

Answer 12

Neonates and blood donors

Question 13

Compare/contrast IAT and DAT

Question 14

How are antibodies are acquired/formed and examples?

Answer 14

Naturally occurring (ABO)
Passively (through mom)
Immune (infection creates memory)

Question 15

What cells will be your positive control for antigen typing?

Answer 15

Heterozygous cells (weakest reaction)

Question 16

What select cells are needed to rule out an antibody?

Answer 16

Cells that are positive for the antibody you want to rule out and negative for the antibody you want to rule in

Question 17

Forward type: A
Back type: AB
What is the discrepancy and how would you resolve it?

Answer 17

Weak/missing reaction - incubate plasma and reagents at RT for 15 minutes

Question 18

Miller Run steps

Question 19

Frequency of K antigen in the population

Answer 19

About 10%

Question 20

Post-transfusion tests

Answer 20

ABORh and DAT poly