Blood and Circulatory System Flashcards
It is a specialized connective tissue in which cells are
suspended in plasma
Blood
it is a fluid extracellular material
Plasma
What is the composition of formed elements?
Red blood cells
White blood cells
Platelets
What is the composition of plasma
albumin a- and b- globulin γ- globulins complement proteins fibrinogen
Most abundant; maintain osmotic pressure
Albumin
For coagulation
a- and b- globulin
Immunoglobulins secreted by lymphocytes
γ- globulins (gamma)
Inflammation and destruction of microorganisms
Complement proteins
Largest plasma protein; block blood loss from small vessels
Fibrinogen
a yellowish liquid produced when plasma proteins react with one another to produce a clot.
Serum
Total blood volume (45%) RBCs in the bottom later
Hematocrit
Refers to the tissue where hemopoiesis takes place or the production of formed elements
Hematopoietic tissue
Organized structures that support immune responses and the sites of lymphocyte development.
Lymphoid tissue
Synonymous with Red Bone marrow from birth onwards.
- Site for the production of all formed elements.
Myeloid tissue
What is the optimal angle to draw blood using capillary
30 - 40 degrees
If the angle is greater than 30 then
smear thicker (low hematocrit)
If the angle is lower than 30 then
smear thinner (high hematocrit)
Are universally employed for staining of blood films.
Romanowsky stains
Fixative
Methanol
The basic dye and has affinity for acidic component of the cell
Methylene Blue
The acidic dye and has affinity for basic component of the cell
Eosin/Azure
What is the the acidic component of the cell
Nucleus
what is the basic component of the cell
Cytoplasm
What are the romanowsky stains
methanol
methylene blue
eosin or azure
It is the mixture of giemsa powder, glycerine and methyl alcohol
Giemsa stain
Dipping of air-dried smear in undiluted stain for 15 to 30 secs
Wright’s stain
This cell lacks nuclei, biconcave discs
Red Blood Cells
What is the lifespan of RBC
120 days
This cell is very small, non- nucleated, membrane- bound cell fragments
Platelets
What is the life span of platelets
9-12 days
Why are WBC important?
Chemotaxis
Chemotactic factors
Diapedesis
Phagocytosis
AZUROPHILIC PRIMARY GRANULES are also known as
lysosomes
It is dense vesicles with a major role in both killing and degrading engulfed microorganism
Azurophilic primary granules
What are the components of azurophilic primary granules
MYELOPEROXIDASE (MPO)
LYSOZYME
DEFENSINS
It is smaller and less dense, stain faintly pink.
Secretion of various ECM degrading enzymes
Specific secondary granules
This cells have nuclei having 2 to 5 lobes linked by thin nuclear extension
Kill and phagocytose bacteria
Neutrophils
What is the lifespan of neutrophil in the blood
8 hours
What is the life span of neutrophil in the tissue
1-4 days
What is the reference range of neutrophils in the body
50% - 70%