Biuret Test Flashcards
1
Q
Outline how you would carry out a Biuret test
A
1) Add NaOH
2) Add drops of copper sulfate
3) Colour change from blue to purple
2
Q
What colour will be observed is protein is present in the sample being tested?
A
Purple
3
Q
Why does a purple colour form?
A
NaOH provides alkaline conditions
Peptide bonds form a complex with Cu2+
