Biotechnology

Question 1

Plasmid

Answer 1

DNA that can provide a selective advantageoften used as a “cloning vector”

Question 2

Restricting Enzymes

Answer 2

bacterial enzymes that cut wherever a specific nucleotide sequence occurs

Question 3

DNA ligase

Answer 3

binds together the “sticky ends” of DNA strands

Question 4

Recombinant DNA

Answer 4

composed of DNA from two or more organisms

Question 5

Selective Markers

Answer 5

can be used to see which cells have been transformedex) antibiotic resistance, color change

Question 6

humilin

Answer 6

genetically engineered human insulin

Question 7

What is used to produce humilin?

Answer 7

genetically altered E. Coli

Question 8

human immune systems developed antibodies against what? what was the result?

Answer 8

bovine and porcine insulininflammatory response at injection site

Question 9

BONUS: who is Dr. Paul Berg

Answer 9

widely regarded as a pioneer in genetic engineeringwon the Nobel Prize in 1980

Question 10

Bt Toxin

Answer 10

Bacillus thuringiensis bacteria produces a protein toxic only to insect larvae

Question 11

what is the problem with Bt corn?

Answer 11

it is not approved by the FDA for human consumption

Question 12

StarLink Corn

Answer 12

contains a protein known as Cry9C (natural Bt toxin)

Question 13

Cry9C

Answer 13

protein that is not easily digested so it could possibly be an allergen in humans

Question 14

Golden Rice

Answer 14

gene for beta carotene production was transferred from a daffodil into a rice plant

Question 15

beta carotine is the precursor to what?

Answer 15

production of Vitamin A

Question 16

Short Tandem Repeats

Answer 16

multiple copies of a short DNA sequence that follows one another along a chromosomenumber and distribution are unique among all individuals

Question 17

Gel Electrophoresis

Answer 17

reveals the DNA fingerprint/ sequence of short tandem repeatselectric field pulls all DNA fragments through a semi-solid gel according to their length as they migrate