Biotechnology

Question 1

Is it easier to develop vaccines for DNA or RNA viruses?

Answer 1

DNA

Question 2

how would you treat megaloblastic marrow?

Answer 2

Folate & B12 mixture

Question 3

What causes severe combined immune deficiency (SCID)?

Answer 3

Adenosine Deaminase (ADA) deficiency. Leads to inability for DNA synthesis, immune system most affected

Question 4

What does the Adenosine Deaminase enzyme do?

Answer 4

Converts adenosine to inosine

Question 5

What is the sequence of nucleotides/genes you want to construct for gene therapy?

Answer 5

LTR-Enhancer-Promoter-Desired cDNA-Poly A signal-LTR

Question 6

What causes chronic myelogenous leukemia (CML)?

Answer 6

a translocation between chromosome 9 and 22. ABL from 9 combines with BCR from 22. Creates BCR-ABL tyrosine kinase.

Question 7

What does BCR-ABL Tyrosine kinase do?

Answer 7

Phosphorylates everything…Rogue kinase! causes cancer

Question 8

WHat is Gleevec?

Answer 8

a pill that acts as a allosteric inhibitor of BCR-ABL tyrosine kinase. Designer drug.

Question 9

What happens when Ras Protein mutates and loses its GTPase activity?

Answer 9

A constant signal to divide = Cancer

Question 10

What is the preferred substrate for DICER?

Answer 10

double-stranded RNA

Question 11

What activates Ras Protein?

Answer 11

MAP Kinase