Biotechnology Flashcards
1
Q
What is DNA sequencing?
A
- Process by which base pairs in DNA strand are determined
- Identifies the sequence of genes that are expressed
2
Q
What tools does DNA sequencing use?
A
- DNA polymerase
- DNA
- Primers
- dNTP’s
3
Q
What is the dideoxy chain termination method?
A
- First sequencing technique to be automated
- Uses modified nucleotides (ddNTPs) to terminate DNA synthesis at specific points
4
Q
What are ddNTPs?
A
- Dideoxy-ribonucleotides
- Mimic regular nucleotides but terminate DNA strand extension
- Each type of ddNTP is tagged with a unique fluorescent label
