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Neet 2024 > Biotechnology > Flashcards

Biotechnology Flashcards

1
Q

Define restriction endonuclease and who found them?
Aka molecular scissors

A

They restrict the growth of virus/ bacteriophage in bacteria.
Isolated in 1963 by arber nathans and smith

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2
Q

Seq of ECOR1

A

5’ GAATTC 3’

Staggered

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3
Q

More than 900 restriction enzymes have been isolated from how many different strains of bacteria?

A

230

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4
Q

First restriction enzyme to be isolated was?

A

HIND 2 (II)

5’GTTACC3’

haemophillus influenza

Blunt ends

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5
Q

First recombinant plasmid?

A

Stanley Cohen and Herbert Boyer in 1972

Native plasmid of salmonella typhimurium was inserted into E Coli ( resistance to streptomycin)

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6
Q

Father of genetic engineering

A

Paul berg

SV-40 ( simian virus ka gene was inserted into E Coli with the help of bacteriophage lambda

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7
Q

On hydrolysis of x-gal (substrate) blue colour is seen in which?

A

Transfomant non recombinant

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8
Q

Agrobacterium tumefacians ke baare mein batao

A

Causes crown gall disease in dicots. It is a gram negative bacteria
It has Ti (tumor inducing) plasmid.

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9
Q

In Ti plasmid.. which region is essential for transfer and integration of T- dna ( transfer dna) into plant cells?

A

Vir region / virulence region.

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10
Q

Wat are retro viruses?

A

Used to infect animal cells

Can cause cancer

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11
Q

Shuttle vectors?

A

Have 2 ori sites -
One for eukaryotes and one for prokaryotes

Eg- yeast episomal plasmid (YEp)

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12
Q

Why is chilled ethanol used in isolation of dna

A

Jab chilled ethanol ko daalenge to dna precipitates out and via spooling ham use remove kr skte

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13
Q

Dna fragments in agrose gel media move towards?

A

Anode (+)

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14
Q

Wat is pcr?

A

Dna replication on a large scale!

Pcr- polymerase chain reaction

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15
Q

Wat is a competent host?

A

Host which has the ability to pick up the r-dna

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16
Q

Golden rice is enriched in?

A

Vitamin A

17
Q

Protein coded bt cry 1 Ab controls?

A

Corn borer

18
Q

Protein coded by cry 1 Ac and cry 2 Ab control?

A

Cotton bollworms

19
Q

Examples of lepidopterans

A

Tobacco budworm
Armyworm

20
Q

Coelpterons

A

Beetles

21
Q

Dipeterans?

A

Flies and mosquito

22
Q

The bt toxin is coded by?

A

Cry gene.. cry 1 Ac

23
Q

Wat is Meloidegyne incognitia?

A

A nematode which infects the roots of tobacco plants

24
Q

State t/f

RNAi takes place in some specific eukaryotes as a method of physiological defence

A

FALSE..

it takes place in all eukaryotes as a method of cellular defence

25
Q

Which vectors were used to introduce nematode specific genes into host plant?

A

Agrobacterium vectors

26
Q

In mammals including humans insulin is synthesized as

A

Pro hormone.. ie it needs to be processed before it becomes fully functional

27
Q

Wat is not present in the mature insulin?

A

C-peptide is not present in the mature insulin.

28
Q

Wat was the main challenge for production of insulin using rdna technique?

A

Getting insulin assembled into a mature form.

29
Q

Which enzyme is crucial for the immune system to work?

A

ADA( adenosine deaminase)

30
Q

Wat are the conventional methods of diagnosis?

A

Serum and urine analysis

31
Q

ELISA is based on which principle?

A

Antigen-antibody interaction

ELISA- Enzyme Linked Immuno-sorbent Assay

32
Q

Which sequence is responsible for controlling the copy number of the linked dna

A

Ori

33
Q

Dna is hydrophilic molecule true / false

A

TRUE

34
Q

Wat helps in identifying non transformants

A

Selectable markers

35
Q

A procedure through which a piece of dna is introduced in a host bacterium

A

Transformation

36
Q

Useful selectable marker for E COLI are?

A

Genes encoding resistance to antibiotics such as

Ampicillin
Chloramphenicol
Tetracycline
Kanamycin

37
Q

ELISA is not an application of pcr.. wat all arre its application???

A

Diagnosis of pathogens
Paleontology
Diagnosis of sickle cell anemia

Neet 2024 (9 decks)

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