biotechnology Flashcards

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1
Q

the two core techniques in biotechnology

A

genetic engineering and bioprocess engineering

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2
Q

techniques of genetic engineering

A

creation of rDNA, gene cloning, and gene transfer

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3
Q

Who found rDNA technology?

A

Stanley Cohen and Herbert Boyer, in 1972

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4
Q

Kanamycin resistance was naturally found in

A

salmonella typhimurium

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5
Q

restriction endonuclease was found in the year

A

1963

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6
Q

first isolated restriction endonuclease

A

Hind 2

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7
Q

recognition sequence consists of how many base pairs

A

6

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8
Q

how many restriction enzymes are known at present

A

900

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9
Q

From how many strains of bacteria have restriction enzymes been isolated?

A

over 230

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10
Q

recognition site of EcoR1

A

GAATTC

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11
Q

Cla 1

A

Caryophanon latum

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12
Q

Hind 3

A

Haemophilus influenzae

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13
Q

Bam H1

A

Bacillus amyloliquefaciens

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14
Q

Sal 1

A

Streptomyces albus

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15
Q

PVU 1

A

proteus vulgaricus

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16
Q

Pst 1

A

Providencia stuartii

17
Q

Recognition site of Cla 1

A

ATCGAT

18
Q

Recognition site Hind 3

A

AAGCTT

19
Q

Recognition site Bam H1

A

GGATCC

20
Q

Recognition site Sal 1

A

GTCGAC

21
Q

Recognition site PVU1

A

CGATCG

22
Q

Recognition site PVU2

A

CAGCTG

23
Q

Recognition site Pst 1

A

CTGCAG