Biosafety Cabinets Flashcards
Removes toxic chemicals
Fume good
Ducting sys. Or ductless
Fume hoods
Fume hood has hepa filler
False fume hood has no hepa filter
Fume hood is for biohazard agents.
True
Product protection. No personnel protection
Laminar flow cabinet
Not for biohazard agent o chemical fumes
Laminar flow cabinets
Personnel and environment protection
Class I BSC I personnel and environment protection
Personnel, product and environment protection
Class l1 and Ili BBC
Not for chemical vapors
Hepa filters
provide effective primary containment for work with infectious material or toxins when they are properly maintained and used in conjunction with good laboratory techniques
BsC
Continuous stream of inward air
Inflow
Helps prevent aerosol from escaping through opening
Inflow
The _______which is exhausted into the surrounding containment zone or directly to the outside atmosphere, is _____to protect the environment
exhaust air, HEPA-filtered
Meaning of hepa filter
High efficiency particulate air
Ulpa filler
Ultra low penetration air
Important definitions:
- HEPA: ___% - at ___ microns
ULPA: ____% - at ___ microns
Important definitions:
- HEPA: 99.99% - at 0.3 microns
- ULPA: 99.999% - at 0.12 microns
The “classical” definition of HEPA filter is ___ at ____ microns, but nowadays all BSC and LF in US use ____% at ___vm
The “classical” definition of HEPA filter is 99.97% at 0.3 microns, but nowadays all BSC and LF in US use 99.99% at 0.3 m
I micron =
I millionth of a meter or 1/ 126.000 of an inch
Hepa / Ulpa capability
Removes a broad range of airborne contaminants
Can affect integrated circuit speed
Impurity con
100% Exhaust
• Inflow velocity 75 fpm minimum
• BSL 1 –3 Usage
Class I BSC
Negative-pressureventilatedcabinet
Class ll BsC
• 30% Exhaust, 70% Re- circulate
Inflow velocity 75 fpm minimum
Class IL type al
30% Exhaust, 70% Re-circulate
Inflow velocity 100 fpm minimum
Class ll type a2