biology part 1 Flashcards
microscopes- prokaryotes
what is the formula for microscopy
measured size/ actual size
m= i/a
what is the function of the course focus
An unclear focus
what is the function of the fine focus
a clear view
what is the function of the objective lens
brings the cell to focus
what is the function of the stage
Supports the specimen
what is the function of the light source
A source of light that helps sees the specimen clearer
what are the disadvantages of using a light microscope
the wavelength of the light limits the microscope from seeing inside of the specimen.
what is the formula for total magnification
magnification of the eyepiece lens X magnification of the objective lens.
what magnification does the eyepiece usually have
x10
what is the greatest magnification of the objective lens
x100
what is the greatest total magnification
x1000
How do you set up a specimen
- the specimen must be thin so that a light/ electron beam can pass through.
- a coverslip is needed to protect the specimen and lens from touching.
- Stains can help distinguish different features of the specimen.
what are the disadvantages of using an electron microscope
it can only examine dead specimens,
what are the advantages of using an electron microscope
it has a shorter wavelength allowing us to see more details in the specimen
what is the formula for actual size
Measured size/ magnification
what structures can a light microscope see
DNA
Plasma membrane
ribosomes
cytoplasm
what structures can an electron microscope see
Plasma membrane Rough ER Smooth ER Mitrochondia Cytoplasm ribosomes golgi apparatus lysomome centrioles nucleus vesicle nucleolus
what is the function of the Plasma membrane
- protects the cell from its surroundings
- regulates the movement of substances in and out of the cell.
what is the function of the Rough ER
- series of single, flattened sacs
- surrounded by ribosomes on the surface.
- proteins made here
what are prokaryotes
bacteria, they are made of single cells with no membrane-bound organelles
what is the function of the smooth ER
- A series of single, sacs made of membrane.
- lipids are made here.