Biology Exam 4 Flashcards
What does PCR stand for?
Polymerase chain reaction
PCR amplifies DNA sequences.
How does PCR work?
In a thermocycler, DNA is denatured, and Taq polymerase adds nucleotides using separated strands as templates.
How can PCR be used in criminal cases?
Creates multiple copies of DNA sequences, making it easier to work with.
What is DNA profiling?
A technique using differences in DNA sequences to positively identify individuals.
What are STRs?
Short tandem repeats, 13 DNA sequences carried by all humans.
How has DNA profiling contributed to the exoneration of individuals?
Positively identifies suspects using DNA.
What is gel electrophoresis?
Separation of DNA fragments by size using an electric current.
How does gel electrophoresis work?
Copied STRs are added to agarose gel, and an electric current is applied; smaller fragments move more quickly.
What is implicit bias?
A subconscious framework affecting feelings based on physical observations.
How can implicit bias impact a jury member?
It can lead to wrongful convictions.
What is gene expression?
Parts of the genome (DNA) being physically shown.
What occurs during transcription?
DNA is converted to RNA in the nucleus.
What is the role of RNA polymerase in transcription?
Binds to the promoter region, unwinds DNA, and ties RNA nucleotides with complementary DNA nucleotides.
What occurs during translation?
RNA is converted to protein at ribosomes.
How does tRNA function in translation?
Matches its anticodon with codons on the mRNA and attaches amino acids.
What is the central dogma theory?
DNA encodes proteins through transcription and translation.
What is a mutation?
Changes in genetic sequences.
What is a substitution mutation?
Substitution of one base for another.
What is a frameshift mutation?
Addition or deletion of a base affecting the reading frame.
Why are some genes expressed in some cell types but not others?
Cells express different genes based on their functions.
What is cloning?
Making copies of the same gene.
How is cloning used in bacteria?
By inserting a gene into a plasmid to be reinserted back into the bacteria.
What is rBGH?
Recombinant bovine growth hormone.
How is rBGH milk made?
Genes with altered rBGH are inserted into the cow.