Biology Exam 4 Flashcards

1
Q

What does PCR stand for?

A

Polymerase chain reaction

PCR amplifies DNA sequences.

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2
Q

How does PCR work?

A

In a thermocycler, DNA is denatured, and Taq polymerase adds nucleotides using separated strands as templates.

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3
Q

How can PCR be used in criminal cases?

A

Creates multiple copies of DNA sequences, making it easier to work with.

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4
Q

What is DNA profiling?

A

A technique using differences in DNA sequences to positively identify individuals.

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5
Q

What are STRs?

A

Short tandem repeats, 13 DNA sequences carried by all humans.

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6
Q

How has DNA profiling contributed to the exoneration of individuals?

A

Positively identifies suspects using DNA.

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7
Q

What is gel electrophoresis?

A

Separation of DNA fragments by size using an electric current.

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8
Q

How does gel electrophoresis work?

A

Copied STRs are added to agarose gel, and an electric current is applied; smaller fragments move more quickly.

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9
Q

What is implicit bias?

A

A subconscious framework affecting feelings based on physical observations.

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10
Q

How can implicit bias impact a jury member?

A

It can lead to wrongful convictions.

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11
Q

What is gene expression?

A

Parts of the genome (DNA) being physically shown.

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12
Q

What occurs during transcription?

A

DNA is converted to RNA in the nucleus.

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13
Q

What is the role of RNA polymerase in transcription?

A

Binds to the promoter region, unwinds DNA, and ties RNA nucleotides with complementary DNA nucleotides.

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14
Q

What occurs during translation?

A

RNA is converted to protein at ribosomes.

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15
Q

How does tRNA function in translation?

A

Matches its anticodon with codons on the mRNA and attaches amino acids.

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16
Q

What is the central dogma theory?

A

DNA encodes proteins through transcription and translation.

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17
Q

What is a mutation?

A

Changes in genetic sequences.

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18
Q

What is a substitution mutation?

A

Substitution of one base for another.

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19
Q

What is a frameshift mutation?

A

Addition or deletion of a base affecting the reading frame.

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20
Q

Why are some genes expressed in some cell types but not others?

A

Cells express different genes based on their functions.

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21
Q

What is cloning?

A

Making copies of the same gene.

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22
Q

How is cloning used in bacteria?

A

By inserting a gene into a plasmid to be reinserted back into the bacteria.

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23
Q

What is rBGH?

A

Recombinant bovine growth hormone.

24
Q

How is rBGH milk made?

A

Genes with altered rBGH are inserted into the cow.

25
What are GMOs?
Genetically modified organisms.
26
What are concerns regarding GMOs?
Interactions with wild populations and competition.
27
What are stem cells?
Unspecialized, undifferentiated precursor cells with an undetermined function.
28
What is gene therapy?
Replacement of defective genes with functional genes.
29
What is biological evolution?
Process by which organisms change over generations through variations in traits and natural selection.
30
What are nonevolutionary changes?
Changes due to short-term environmental factors that are not genetic.
31
What is the theory of common descent?
The theory that all living organisms share a common ancestor.
32
What do phylogenetic trees illustrate?
How different species are related through one common ancestor.
33
What influenced Darwin's theory of evolution?
Collection of diverse animals and plants, Principles of Geology, divergence between island organisms and mainland species.
34
What is the Linnean system?
A classification system based on shared traits and evolutionary relationships.
35
What are the major domains of life?
* Bacteria * Archaea * Eukarya
36
What is anatomical homology?
Similarities in skeletal bone structures.
37
What is convergent evolution?
Structural similarities in unrelated species with relevant lifestyles.
38
What are vestigial traits?
Nonfunctional or greatly reduced traits.
39
What is biogeography?
The distribution of species on Earth.
40
What is radiometric dating?
Using ratios of radioactive elements to estimate the age of rocks.
41
Why is the theory of evolution the best explanation for the origin of humans?
It is well-supported by a wide variety of evidence, including anatomy, geology, molecular biology, and genetics.
42
What is the biological species concept?
A group of individuals that can interbreed and produce fertile offspring.
43
What is reproductive isolation?
Prevention of gene flow between species.
44
What are pre-fertilization barriers?
Barriers that prevent fertilization from occurring.
45
What is spatial reproductive isolation?
Individuals from different species do not come in contact with each other.
46
What is hybrid inviability?
Zygote cannot complete development due to incomplete genetic instructions.
47
What are the three steps of speciation?
* Isolation of gene pools * Evolutionary changes in gene pools * Evolution of reproductive isolation
48
What is the evidence that modern humans arose in Africa?
Humans have less genetic diversity than other great apes, and African populations have the greatest genetic diversity.
49
What is genetic drift?
Change in allele frequency due to chance.
50
What is the founder effect?
Genetic differences resulting when a small sample begins a new population.
51
What is sexual selection?
When a trait influences the likelihood of mating.
52
What is assortative mating?
Preference to mate with someone similar (e.g., height).
53
What is adaptive radiation?
Rapid diversification of some species into a large and varied group of descendant species.
54
Why are viruses considered nonliving?
They cannot maintain homeostasis and are incapable of growth or reproduction without assistance.
55
How are evolutionary classifications of living organisms created?
Using phylogeny and cladistic analysis.