Biology Flashcards
Describe cell theory.
The cell theory states that all living things are composed of cells; cells are the basic functional unit of life; cells arise only from pre-existing cells; and cells carry their genetic information in the form of DNA.
Name the two distinct groups into which all cells can be categorized?
All cells can be categorized as either prokaryotes or eukaryotes.
What is the key differentialting criterion between prokaryotic and eukaryotic cells?
Eukaryotic cells have membrane-bound organelles; however, prokaryotic cells do not.
T/F, bacteria and viruses are examples of prokaryotic cells.
False, bacteria are prokaryotic while viruses are non-living acellular structures.
Describe bacterial DNA?
Bacterial DNA consists of a single circular chromosome.
What is a plasmid?
A plasmid is a smaller extrachromosomal ring of DNA sometimes found in bacteria. It replicates independently of the bacterial chromosome.
T/F, bacteria contain ribosomes?
True, but prokaryotic ribosomes are smaller than eukaryotic ribosomes.
Name the components of a typical bacterial cell?
Cell wall, cell membrane, cytoplasm, ribosomes, flagella, and DNA.
Where does respiration occur in the bacterial cell?
The cell membrane is the site of respiration in bacteria.
T/F, all multicellular organisms are composed of eukaryotic cells?
True.
Which type of eukaryotic cells haave a cell wall?
Plant cells and fungal cells have a cell wall.
What is cytosol?
Cytosol is the fluid component of the cytoplasm.
What are the primary components of the cytoskeleton?
The primary components of the cytoskeleton are microtubules, microfilaments, and intermediate fibers.
Define the fluid mosaic model?
The fluid mosaic model states that a cell membrane consists of a phospholipid bilayer with proteins embedded throughout.
Is the interior of a cell memebrane hydrophilic or hydrophobic?
The interior of a cell membrane is hydrophobic.
What are the very tips facing outward called on the phospholipid bilayer?
Polar heads.
What are the inner tails of the phospholipid bilayer called?
Non-polar tails.
What are the function of transport protein?
A transport protein helps move polar molecules and certain ions across the cell membrane.
What is a memebrane receptor?
A membrane receptor is a protein (or glycoprotein) that binds to molecules in the extracellular environment.
Can small polar and non-polar molecules easily cross the cell membrane?
Yes, because of their size, small polar and non-polar molecules can easily traverse the cell membrane.
How does a large charged molecule cross the cell memebrane?
A large charged particle usually crosses the cell membrane with the help of a carrier protein.
T/F, the nucleus is surrounded by a single-layered memebrane?
False, the nuclear membrane is double-layered.
How is material exchanged between the nucleus and the cytoplasm?
The nuclear membrane contains nuclear pores that selectively allow for the exchange of materials.
What is a histone?
A histone is a structural protein complexed with eukaryotic DNA to form a chromosome.
What is the function of the nucleolus?
The nucleolus synthesized ribosomal RNA (rRNA).
What is the function of a ribosome?
A ribosome is the site of protein translation (assembly) during protein synthesis.
What is the general function of the endoplasmic reticulum?
Endoplasmic reticulum is involved in the transport of material throughout the cell.
What is the function of smooth endoplasmic reticulum (smooth ER)?
Smooth ER is the site of lipid synthesis and poison detoxification, and it is involved in protein transport within the cell.
What is the function of rough endoplasmic reticulum (RER)?
RER serves as an attachment point for ribosomes, which functions in protein synthesis for membrane bound proteins and proteins to be excreted from the cell.
T/F, proteins synthesized by RER are secreted directly into the cytoplasm?
False, they are secreted into the cisternae of RER and then sent to smooth ER, where they are secreted into vesicles.
What is the function of the Golgi apparatus?
The Golgi apparatus receives vesicles from smooth ER, modifies them, and repackages them into vesicles for distribution.
What happens to a secretory vesicle after it is released from the Golgi apparatus?
A secretory vesicle from the Golgi fuses with the cell membrane to release its contents via exocytosis.
T/F, vesicles and vacuoles are membrane-bound sacs involved in transport and storage of cellular materials?
True.
What is a lysosome?
A lysosome is a membrane-bound vesicle that contains hydrolytic enzymes involved in intracellular digestion.
T/F, lysosomes fuse with endocytotic vesicles and help digest their contents?
True.
How does the pH in the interior of a lysosome compare with the pH in the rest of the cell?
The interior of a lysosome is acidic and therefore has a lower pH than the rest of the cell.
What would happen if a lysosome released its contents into the cytoplasm?
The hydrolytic enzymes of the lysosome would digest the organelles and kill the cell. This process is known as autolysis.
What is the function of peroxisomes?
Peroxisomes make hydrogen peroxide and digest fats into smaller molecules.
What is the funciton of mitochondria?
Mitochondria are the sites of aerobic respiration and supply most of the cell’s energy.
T/F, mitochondria have their own circular DNA?
True.
Do mitochondria replicate in a manner similar to a cell’s other organelles?
No, they replicate via binary fission.
T/F, mitochondria and cholorplasts are similar structures and both are considered to be semi-autonomous?
True.
What is the function of a cell wall?
A cell wall protects the cell from external stimuli and desiccation.
What is the function of centrioles?
Centrioles are involved in spindle formation during cell replication. They are found only in animal cells.
What is a centrosome?
The centrosome is the region of a cell that contains the centrioles.
What is the function of the cytoskeleton?
The cytoskeleton gives mechanical support, maintains the cell’s shape, and functions in motility.
What is the function of microtubules?
Microtubules maintain cell shape, form the spindle apparatus and provide tracks along which organelles can move.
T/F, cilia and flagella are specialized arrangements of microfilaments and function in cell motility?
False, while cilia and flagella do function in cell motility, they are composed of microtubules.
T/F, microfilaments are solid rods of actin and are involved in cell movement and cell wall support.
True.
T/F, simple diffuction is a passive process that requires energy?
False, simple diffusion is a passive process and therefore DOES NOT require energy.
Define osmosis.
Osmosis is the simple diffusion of water from a region of lower solute concentration to a region of higher solute concentration.
What would happen to a cell if it were put in a hypertonic solution?
A hypertonic solution would cause water to flow out of a cell and cause the cell to shrink.
T/F, a hypotonic solution will cause water to flow into a cell, causing it to swell?
True.
Define isotonic.
A medium and a cell are said to be isotonic when the solute concentrations of the medium and the cell are equal.
Define facilitated diffusion.
Facilitated diffusion is the net movement of dissolved particles down their concentration gradient with the help of carrier molecules.
T/F, facilitated diffusion requies energy?
False, all forms of diffusion, including facilitated diffusion, are passive processes.
Define active transport.
Active transport is the net movement of dissolved particles against their concentration gradient with the help of carrier molecules. This process requires ATP.
T/F, active transport requires energy?
True.
What is endocytosis?
Endocytosis is a process in which the cell membrane invaginates, forming an intracellular vesicle containing extracellular medium.
What is exocytosis?
Exocytosis is a process in which a vesicle within the cell fuses with the cell membrane and releases its contents to the extracellular medium.
What is the difference between pinocytosis and phagocytosis?
Pinocytosis is the endocytosis of liquids and small particles, whereas phagocytosis is the endocytosis of large particles.
What are the four basic types of tissue found in the body?
The four basic types of tissue found in the body are: epithelial, connective, nervous, and muscle.
What are the componenets of a virus?
A protein coat and nucleic acid.
What kinds of nucleic acid are found in viruses?
Single- or double-stranded DNA or RNA.
T/F, viruses are obligate intracelluar parasites?
True.
What is a bacteriophage?
A bacteriophage is a virus that infects bacteria only.
What is the genetic material of a virus?
The genetic material of a virus can be DNA or RNA.
How do enzymes speed up a reaction?
Enzymes are protein catalysts that accelerate a reaction by reducing the amount of activation energy required.
T/F, enzymes get used up during the course of a reaction?
False, enzymes are catalysts and therefore don’t get used up during the course of a reaction.
T/F, enzymes are very selective in the reactions they catalyze?
True.
What is substrate?
A substrate is a molecule upon which an enzyme acts.
What is an active site?
An active site is the area of an enzyme to which a substrate binds.
What is the lock and key theory?
The lock and key theory states that an enzyme and its corresponding active site are exactly complementary.
T/F, the induced fit hyposthesis holds that an enzyme causes a conformational change in its corresponding active site to facilitate substrate binding.
True.
What is a cofactor?
A cofactor is a nonprotein molecule that is incorporated into an enzyme and is required for proper functioning.
What is the difference between an apoenzyme and a haloenzyme?
An apoenzyme is an enzyme without its corresponding cofactor. A holoenzyme contains its cofactor.
What is a coenzyme?
A coenzyme is an organic cofactor for an enzymatic reaction (e.g., vitamin)
What is a prosthetic group?
A tightly bound cofactor is also known as a prosthetic group.
T/F, most coenzymes are synthesized by the body?
False, most coenzymes are obtained from outside sources.
What happens to the free energy (∆G) or a reaction if it is catalyzed by an enzyme?
The free energy of an enzyme-catalyzed reaction is the same as the reaction without the enzyme.
What happens as the concentration of substrate is increased in an enzyme-catalyzed reaction?
The reaction rate increases until most of the active sites are filled and then the reaction rate reaches a plateau.
T/F, at very high concentrations of substrate, reaction rate approximates Vmax?
True.
T/F, all enzymes in the body are proteins?
False, ribozymes are RNA enzymes.
What is the optimal pH for the enzymes of glycolysis?
7
What is the optimial temperature for most enzyme in the body?
37ºC
T/F, all enzymes in the body operate most efficiently at a pH of around 7.2?
False, enzymes in the digestive tract work best at acidic and basic pH’s.
What is an allosteric enzyme?
An allosteric enzyme is an enzyme with two or more active sites. An allosteric enzyme oscillates between an active an inactive configuration.
What happens to an enzyme in the presence of an allosteric inhibitor?
An allosteric inhibitor prevents an enzyme from binding to its substrate by stabilizing the inactive configuration.
What are the three categories of enzymatic regulatory inhibition?
Feedback, reversible, and irreversible inhibition.
How does feedback inhibition regulate an enzymatic proces?
Feedback inhibition uses an end product as an allosteric inhibitor to the enzyme catalyzing the reaction.
What re the two types of reversible inhibitors?
The two types of reversible inhibitors are competitive and non-competitive inhibitors.
T/F, a noncompetitive inhibitor can be overcome by increasing the concentration of substrate?
False, a non-competitive inhibitor doesn’t bind at the active site and therefore doesn’t compete with the substrate.
T/F, a competitive inhibitor binds at the active site and therefore can be overcome by increasing the concentration of substrate?
True, a competitive inhibitor competes with the substrate for active sites.
How can a non-competitive inhibitor’s effect be reversed?
Through the addition of compound having a greater affinity for the inhibitor than the inhibitor has for the enzyme.
T/F, Irreversible inhibition involves permanent damage to the active site?
True.
What is a zymogen?
A zymogen is an inactive form of an enzyme.
How is a zymogen activated? Give some examples of zymogens.
A zymogen is activated when a part of it is cleaved off. A few examples are digestive enzymes such as pepsinogen and chymotrypsinogen. (Note the “ogen” suffix.)
What is an anobolic process?
An anabolic process is a reaction that builds complex molecules and requires energy.
What is a catabolic process?
A catabolic process is a reaction that breaks down complex molecules and releases energy.
What is an autotroph?
An autotroph is an organism that can harness sunlight to create energy via photosynthesis (e.g., plants)
What is a heterotroph?
A heterotroph is an organism that breaks down organic nutrients for energy.
What are the energy carrier molecules for a cell’s metabolic proceses?
ATP, NAD+, NADP+, and FAD
How to ATP, NAD+, NADP+, and FAD store energy?
ATP stores energy in its high-energy phosphate bonds. NAD+, NADP+, and FAD store energy in high-potential electrons.
Does oxidation result in the loss or gain of electrons?
Oxidation refers to the loss of electrons.
What happens to the number of electrons when a reactant gets reduced?
Reduction results in the gain of electrons.
T/F, an oxidizing agent gets oxidized during a reaction?
False, an oxidizing agent gets reduced and thereby causes another molecule to be oxidized.
What is the net reaction for glycolysis?
Glucose + 2ADP + 2Pi + 2(NAD+) ––––> 2Pyruvate + 2ATP + 2NADH + 2(H+) + 2H20
T/F, glycolysis results in substrate level phosphorylation of ADP?
True.
Is glycolysis an aerobic or anaerobic process?
Glycolysis is an anaerobic process.
What are the possible products of fermentation?
The products of fermentation are either ethanol or lactic acid.
When does a cell rely on fermentation of its energy needs?
A cell utilizes fermentation in an anaerobic (oxygen deficient) environment.
What is the net gain in ATP from cellular respiration?
Eukaryotes = 36 ATP; Prokaryotes = 38 ATP.
T/F, cellular respiration requires aerobic conditions?
True.
What are the three stages of cellular respiration?
The three stages of cellular respiration are: pyruvate decarboxylation, the citric acid (Krebs) cycle, and the electron transport chain.
T/F, During pyruvate decarboxylation, a molecule of CO2 is lost, leaving acetyl CoA?
True.
One molecule of glucose requies how many turns of the citric acid cycle?
The citric acid cycle (TCA) completes two cycles (turns) per molecule of glucose.
How many ATP, NADH, and FADH2 are created in one turn of the citric acid cycle?
Each turn of the citric acid cycle results in 1 GTP, 3 NADH, and 1 FADH2.
What is a cytochrome?
A cytochrome is a molecular electron carrier involved in oxidative phosphorylation.
When and where does oxidative phosphorylation occur?
Oxidative phosphorylation occurs at the inner mitochondrial membrane during the electron transport stage of cellular respiration.
Does the electron transport chain require oxygen?
Yes, the final acceptor of the electrons is O2, which then forms water.
T/F, the electron transport chain generates a proton gradient across the innter mitochondrial membrane?
True.
Where does glycolysis occur?
Glycolysis occurs in the cytoplasm.
Where does the citric acid cycle occur?
The citric acid cycle occurs in the inner mitochondrial matrix.
T/F, if glucose is unavailable, the body can use carbohydrates, fats and proteins to create energy?
True, Gluconeugenesis
What are the four stages of the cell cycle?
The four stages of the cell cycle are: G1, S, G2 and M.
When do a cell’s chromosomes replicate?
The chromosomes replicate during the S (synthesis) stage of interphase.
What is the order of the four stages of mitosis?
Prophase, Metaphase, Anaphase, Telophase.
What is a chromatid?
A chromatid is one half of a replicated chromosome. Sister chromatids are attached by the centromere.
In which mitotic phase does spindle formation occur?
Spindle formation occurs during prophase.
In which mitotic phase are the chromosomes lined up in the equatorial plane?
The chromosomes line up during metaphase.
In which mitotic phase do the chromosomes separate?
The chromosomes separate during anaphase.
In which phase does cytokinesis occur?
Cytokinesis occurs during telophase.
How many chromosomes are in a human diploid cell?
A human diploid cell has 46 chromosomes.
How many chromosomes are in a human haploid cell?
A human haploid cell has 23 chromosomes.
What is a gametocyte?
A gametocyte is a haploid cell that undergoes meiosis.
T/F, homologous chromosomes code for different traits.
False, homologous chromosomes code for the same traits.
In what phase of meiosis would one fine a tetrad?
Tetrads exist in Prophase 1.
T/F, synapsis and crossing over result in genetic recombination?
True.
Name the different structures of the male reproductive tract.
SEVEN UP: Seminiferous tubules (testes), epididymis, vas deferens, ejaculatory duct, (nothing), urethra, penis.
What is the sequence of development for a mature sperm cell?
Primary spermatocyte, secondary spermatocyte, spermatid, and spermatoza.
What is an acrosome?
An acrosome is the enzyme-containing cap-like structure on the head of a sperm.
T/F, at birth, a female child’s ova are arrested at prophase 1 and are called primary oocytes?
True.
Starting with the ovary, name the different structures of the female reproductive tract.
Ovary, oviduct (fallopian tube), uterus, cervix, vagina.
What are the corona radiata and zona pellucida?
The corona radiata is the outer layer of cells that surrounds the ovum. The zona pellucida is the inner layer of cells that surrounds the ovum.
What is a polar body?
A polar body is a small cell that results from unequal distribution of cytoplasm during meiosis.
T/F, dizygotic (fraternal) twins are identical?
False, monozygotic twins are identical.
Order the follwing embyonic stages: blastula, neurula, morula, gastrula, zygote.
zygote, morula, blastula, gastrula, neurula.
What is differentiation?
Differentiation is the specialization of cells that occurs during development.
What are the 3 primary germ layers?
Ectoderm, endoderm, and mesoderm.
What is induction?
Induction is the influence of a group of cells upon the differentiation of another group of cells.
When does the inner cell mass first appear?
Blastula.
When does the Archenteron first appear in development?
Gastrula.
When does the Notochord first appear in development?
Neurula.
When does the Trophoblast first appear in development?
Blastula.
When does the Blastopore first appear in development?
Gastrula.
When does the Neural fold first appear in development?
Neurula.
What structures arise from ectoderm?
Ectoderm - integument, lens of the eye, and nervous system.
What structures arise from endoderm?
Endoderm - epithelial linings of digestive and respiratory tracts, parts of liver, pancreas, thyroid, and bladder.
What structures arise from Mesoderm?
Mesoderm - musculoskeletal system, circulatory system, connective tissue, excretory system, and gonads.
T/F, the placenta and umbilical cord are responsible for fetal respiration, nutrition, and waste removal.
True.
T/F, adult hemoglobin has higher affinity for oxygen than fetal hemoglobin?
False, fetal hemoglobin has a higher affinity for oxygen.
What is the function of the ductus venosus?
The ductus venosis shunts blood away from the fetal liver.
What is the function of the foramen ovale?
The foramen ovale diverts blood away from the pulmonary arteries and into the left atrium.
What is the function of the ductus arteriosus?
The ductus arteriosus shunts blood directly from the pulmonary artery into the aorta.
In what trimester does the fetal heart begin to beat?
The heart begins to beat in the first trimester.