Biologics Flashcards
What’a GALT?
Gut-associated lymphoid tissue
- it is a mucosal immune system
- in GIT
Innate immunity vs Adaptive immunity
What cells are affected by cytokines?
- immune cells
- fibroblasts
- endothelial cells
The role of macrophages a cancerous cell that is over-producing itself?
- Macrophages ‘collect’ the sample from a cell that is over-producing itself
- some of the collected cells - would be normal cells - if these are presented to antigen presenting cells/T cell -> then nothing would be done
- If abnormal proteins are presented -> then we can fight the cancer
* Check-Point inhibitors -> will block the pathway of cancer development
What are check-point inhibitors?
They block the pathway of a body not being able to fight cancer (due to ‘normal’ cellular antigens being presented to T cells)
Example of a key cytokine that initiates the pathway in responder cell that is specific to that cell?
RA -> TNF alpha
What are (2) regions on immunoglobulin?
- Fab -> fragment antigen binding
- Fc -> determines the type of immune response
What would the variable regions of immunoglobulin protein mean/result in?
Genetic variability of immunoglobulins - able to match different antigens
What are antigens?
Antigens - proteins on a cell/ micro-organism that are able to activate B cells (and production of antibodies)
How dos elimination of antigen occur? (2)
- directly -> by neutralisation
- indirectly -> by promoting phagocytosis or complement activation (lysis)
IgM vs IgG
- IgM - early stage
- IgG - late
What’s the role of IgE in allergic reaction?
IgE triggers the release of histamine from basophils
What antibody is linked with mucosal immunity?
IgA
What is the function of IgD
cellular regulatory function
What most biologics are?
Antibodies
How are monoclonal antibodies produced?
- Antigens are injected into the mouse -> mouse will produce antibodies in spleen
- Antibody-producing cells from the spleen - but they do not have long lives - as cells die
- We want to make the cells immortal -> so we fuse them with tumour cells (immortal) -> hybridomas are formed
- Hybridomas produce antibodies -> these can be injected into patient and block specific pathways
*we combine human and mouse immunoglobulins - to reduce allergic reaction to animal antibody (chimeric antibody)
* most recent technologies -> human antibodies only are produced
What are biologics?
Medications with near-identity to the original biologic agents (e.g. protein or antibody
What are the problems with biologics?
We are not able to monitor entirely how the antibodies circulate and move around the body - not predictable pharmacokinetics and pharmacodynamics
What are biosimilars?
Type of biologics