biological molecules Flashcards
what do carbohydrates, proteins and lipids contain, give examples of each
carbohydrates: CHO carbon, hydrogen, oxygen
eg. glucose, glycogen, starch, cellulose
proteins: CHON (S) carbon, hydrogen, oxygen, nitrogen, sometimes sulphur
eg. enzymes, hormones, blood proteins
lipids: CHO carbon, hydrogen, oxygen
eg. fats, oils
what determines how the proteins function
the different amino acids and their structure can determine the shape, size, properties of the protein and how it folds in different ways
protein food test
biuret test
neg- blue
pos- purple
- half fill test tube with extract you wish to test
- add 5-6 drops of biuret solution (contains copper sulfate solution and sodium hydroxide solution)
Safety: sodium hydroxide is corrosive - if color remains blue: no protein; if color turns purple: contains protein
lipids food test
ethanol emulsion test
fats will not dissolve in water but will dissolve in ethanol, if a solution of fat in ethanol is added to water, a cloudy white emulsion is formed
- grind/chop a small amount of the material you wish to test
- put the extract into a test tube and add enough ethanol to cover it
- put a stopper on the test tube and shake the contents
- add distilled water until the test tube is half full, then repeat 3
- if cloudy white emulsion formed- contains fat: does not happen, does not contain fat
starch food test
iodine test
- half fill a test tube with food extract then add 2-3 drops of iodine solution
- positive: blue-black, contains starch ; negative: remains yellow/light brown
reducing sugars test
Benedict’s test
- place the sample in a test tube
- set up a hot water bath: beaker on heat proof mat, half-fill a beaker with boiling water/ place beaker on tripod and gauze and boil water with Bunsen burner
- add the same volume of Benedict’s solution to the test tube containing the food extract, put tube in hot water bath
- Positive test: red/orange (look carefully you see it turn green and yellow before orange), if you leave it to cool you will see a precipitate
green: only a little reducing sugar present
deep orange: lots of reducing sugars
negative: blue, does not contain any
vitamin C test
DCPIP test
DCPIP is a blue liquid that loses its color when it comes into contact with vitamin C
- put a known volume of DCPIP solution in a test tube
- fill a syringe/dropping pipette with a solution of vitamin C or juice (eg. ogjuice)
- add the liquid one drop at a times
- if color of DCPIP disappears: test is positive
if blue color persists then the test is negative
Vit C decolourises DCPIP solutions
biological molecules are _____ _____ like _____, _____ and _____
they are useful chemicals needed by _____ _____ for _____
by _____ we mean all the _____ _____ taking place in the _____ of the body
these reactions include the release of _____ in _____, _____ _____, and the _____ and _____ of cells
biological molecules are complex chemicals like carbohydrates, proteins and fats
they are useful chemicals needed by living organisms for metabolism
by metabolism we mean all the chemical reactions taking place in the cells of the body
these reactions include the release of energy in respiration, protein synthesis, and the growth and repair of cells
what do green plants use simple raw materials for?
green plants make the complex chemical compounds that they need from simple raw materials
CO2 and H2o are the raw materials for photosynthesis
simple sugars produced in photosynthesis used to make a wide range of other compunds
- plants need minerals to make some of these complex compounds
the different biological molecules needed for a balanced diet in humans are what?
carbohydrates, proteins, fats, vitamins, minerals, fibre and water
describe carbohydrates
what is cellulose used for
what is starch used for
what is glycogen used for
- made of many sugar molecules containing CHO atoms
- glucose is a small sugar molecule
when many glucose bind together in a chain, cellulose, starch and glycogen can be made
cellulose is used for structure and makes up plant cell walls
starch and glucose are stored in cells and used in respiration to provide energy for the organism
starch is stored in PLANTS
glycogen is stored in ANIMALS
describe fats/lipids
- made up of glycerol and fatty acids which contain CHO
- have a variety of roles in organisms: insulation, energy, waterproofing, structure and protection around delicate organs
describe proteins
- made up of CHON(S), these elements make up amino acids which bind together in a chain to make proteins
- different arrangements of amino acids make up different proteins and form different shapes
- enzymes are proteins; each enzyme active site has a specific shape
- antibodies also have a specific shape, formed by a specific sequence of amino acids
- this allows them to bind to antigens on foreign pathogens to kill them
why is water an important solvent
- its an important molecule which is a major component of cells
- acts as solvent in which many chemical reactions occur, helps to maintain a constant temperature in the body and is a metabolite
why is water needed for digestion
needed for digestion to provide a medium for enzymes to act in and to allow free passage of digestive products, so they can be absorbed into the blood
- allows soluble molecules, such as products of digestion to be transported around the body and through cell membranes
transports waste products such as urea and carbon dioxide to be excreted
what smaller molecules are starch and glycogen made from?
glucose
what smaller molecule is cellulose made of
glucose
what smaller molecules are proteins made of
amino acids
what smaller molecules are fats and oils made from
fatty acids
glycerol
give 2 functions of proteins
antibodies
enzymes