Biological molecules Flashcards
what is the bond between two monosaccharides
glycosidic bond
maltose-
sucrose-
lactose-
maltose- two glucose
sucrose- glucose and fructose
fructose- galactose and glucose
Structure and role of glycogen
contains many alpha glucose with the 1,4 and 1,6 glycosidic bonds
- energy storage in animals
- large and compact
- insoluble so doesn’t affect water potential
structure and role of starch
contains the alpha glucose amylose and amylopectin
- amylose- 1,4 and 1,6 glycosidic bond and is unbranched. Coiled so compact to store energy
- amylopectin- 1,6 glycosidic bond, branched
- energy storage in plants
- doesn’t effect water potential
Benedict’s test
- food in liquid form
- Benedict’s reagent
- heat mixture gently in water bath
- brick red means reducing sugar
- if blue add HCl to hydrolyse the non reducing sugar
- add NaOH to neutralise it
then add Benedict’s again and the colour should change to brick red
Triglycerides
- formed by the condensation of one molecule of glycerol and three fatty acids
- large and non polar
- does not affect water potential of cells when stored
- important water source for organisms in dry places
Phospholipids
- one fatty acids of a triglycerides is substituted by a phosphate containing group
- polar so bilayer can form
- hydrophilic head
- structure important for cell recognition
Emulsion test
- liquid food sample
- ethanol and shake
- add water
cloudy = lipid
Primary structure
the order of amino acids
secondary structure
mainly arranged as alpha helix or beta pleated
tertiary structure
secondary but more folded and has 3D structure and contains 3 different bonds
disulphide bridges
not easily broken
hydrogen bonds
numerous and easily broken
ionic bonds
between the carboxyl group and amino group that are not involved in peptide bond, easily broken by pH change
Biuret test
- sample in test tube
- add NaOH
- few drops of very dilute copper sulfate solution and mix gently
- purple means there is peptide bond