Biological membranes Flashcards
what are membranes
partially permeable barriers that allow cell compartmentalisation, resulting in different conditions either side.
why are membranes defined as fluid mosaic
- phospholipid molecules are constantly moving
- embedded with proteins : glycoproteins (proteins that have a carbohydrate chain)
- glycolipids: lipids that have a carbohydrate chain attached
- cholesterol
description of phospholipids in the plasma membrane
- forms a bilayer: hydrophilic phosphate heads oriented towards water, hydrophilic fatty acid tails oriented inwards away from water
- lipid-soluble substances can pass
role of proteins in plasma membrane
act as receptors, channels, carriers. Can support membrane and cytoskeleton
role of cholesterol in plasma membrane
- regulates membrane fluidity and provides strength by binding to hydrophobic tails causing them to pack closely together
role of glycoproteins/glycolipids
- receptors for cell signalling
- antigens
- sites where drugs, hormones and antibodies can bind
- they stabilise the membrane by forming H bonds with surrounding water molecules
effect of low temp on plasma membrane
- saturated fatty acids pack more closely together, reducing fluidity and increasing rigidity
- counteracted by unsaturated fatty acids with kinks pushing phospholipids apart and cholesterol
effect of high temp on plasma membrane
- phospholipids gain more Ke and vibrate more, plasma membrane becomes more fluid and permeable
role of plasma membranes
- barrier between cell and its environment, controlling which substances enter or leave
- allow recognition by other cells ( immune system)
- allow cell communication (cell signalling)
role of membranes within cells
- membranes around organelles divide cells into compartments making different functions more efficient like substances needed for respiration being kept inside mitochondria
- formation vesicles to transport substances
- control which substances enter and leave organelle e.g. RNA leaves nucleus via nuclear membrane
- site of chemical reactions like inner membrane of mitochondrion contains enzymes for respiration membranes within organelles act as barriers (thylakoid membrane in chloroplast)
in which membranes is cholesterol not found in
bacterial
effect of high temperature on cytoskeleton
proteins in it denature
how is permeability investigated
- investigated using beetroots and solvents
1. make a serial dilution of solvent using stock solution
2. add beetroot different dilutions
3. measure pigment released into solution from cells using a colorimeter calibrated with water
4. record data and interpret results
effect of destroyed plasma membranes on light transmission in colorimetry
reduced
define diffusion
the passive net movement of molecules from an area of higher conc to an area of lower conc down the conc gradient