Biocompatibility

Question 1

Hazard definition

Answer 1

the actual contact with or entrance into a living system of the toxic age

Question 2

toxic give hazerdous to patient?

Answer 2

no - not always if we can manage it

Question 3

toxicity description

Answer 3

most in dentistry are toxic - yet we dont cause the hazerdous effects

Question 4

three stages of biological tests for dental materials

Answer 4

1. initial tests
2. secondary tests
3. usage tests

Question 5

Breakdown of cytotoxicity tests

4 types

Answer 5

1. cell attachment and growth
2. membrane permeability
3. metabolic activity
4. Systemic toxicity test

Question 6

if see hazerdous effect in step one cytoxicity test?

Answer 6

does not go onto the secondary testing

Question 7

2 examples of tests used for cell membrane permeability

Answer 7

1. 51 Cr release assay
   - chromium release assay
2. neutral red release assay

Question 8

if material is hazerdous what is the effect on permeability

Answer 8

permeability will INCREASE

• can pass through cell membranes

Question 9

test more used to test cell membrane permeability

Answer 9

neutral red release assay

Question 10

neutral red release assay

general description of healthy cell vs injured cell

Answer 10

Healthy cell
- stained pink / red with the neutral red and has INTACT MEMBRANE

WHEN ADD THE MATERIAL TO TEST

IF INJURED CELL? –> permeable membrane and no longer see the pink/ red staining as it is LEACHED OUT