Biochemistry - Molecular Flashcards
What is the structure of chromatin?
“Beads on a string”
Negative DNA wrapped around a positively charged histone octamer –> forms a nucleosome
What phase does DNA and histone synthesis occur?
S phase
Which type of chromatin is condensed and which is less condensed b/t heterochromatin and euchromatin?
Heterochromatin - highly condensed, transcriptionally inactive
Euchromatin - less condensed, transcriptionally active
At what site does DNA methylation occur?
What is a result of DNA methylation?
CpG islands
Methylated CpG islands repress transcription
“CpG Methylation Makes DNA Mute”
Histone methylation
Histone Methylation Mostly Makes DNA Mute
What effect does histone acetylation have on DNA?
Relaxes DNA coiling –> allows transcription
Histone Acetylation makes DNA Active
What are the 2 purines? How many rings do they have?
Adenine, Guanine (PURe As Gold)
2 rings
What are the 3 pyrimidines? How many rings do they have?
Cytosine, Thymine, Uracil (CUT the PY (pie))
1 ring
What structure is uracil found in?
What structure is thymine found in?
DNA vs. RNA
RNA
DNA
What side group does thymine have?
A methyl group
Thymine has a methyl.
What amino acids are necessary for purine synthesis?
GAG
Glycine
Aspartate
Glutamine
NucleoSide = base (deoxy)ribose
Attached to a SUGAR
NucleoTide = base (deoxy)ribose + phosphaTe
Linked by 3’-5’ phosphodiester bond
Which two metabolic pathways is carbamoyl phosphate involved in?
pyrimidine synthesis
urea cycle
What enzyme does the following drug inhibit in nucleotide synthesis?
- Leflunomide
- Mycophenolate, Ribavirin
- Hydroxyurea
- 6-Mercaptopurine (MP) and its prodrug Azathioprine
- 5-Fluorouracil (5-FU)
- Methotrexate (MTX), Trimethoprim (TMP), and pyrimethamine
- dihydroorotate dehydrogenase
- IMP dehydrogenase
- ribonucleotide reductase
- de novo purine synthesis
- thymidylate synthase
- dihydrofolate reductase
Purine Salvage Deficiencies
What disease results from a deficiency in adenosine deaminase?
What is its genetic inheritance pattern?
What molecular findings will you see?
SCID
Autosomal recessive
Excess ATP and dATP –> prevents DNA synthesis –> decreases lymphocyte count
Purine Salvage Deficiencies
What disease results from a deficiency in HGPRT?
What is its genetic inheritance pattern?
What molecular findings will you see?
What physical findings will you see?
What is the treatment?
Lesch-Nyhan Syndrome
X-linked recessive
Overproduction of purines and accumulation of uric acid
Hypersegmented neutrophils*
Intellectual disability, gout, dystonia
Tx: allopurinol or febuxostat (2nd line)
Hyperuricemia Gout Pissed off (aggression, self-mutilation e.g. biting nails) Retardation (intellectual disability) DysTonia (spasticity)
Which two codons are encoded only by 1 codon (AUG and UGG, respectively)?
Methionine and tryptophan
What type of DNA replication occurs in both prokaryotes and eukaryotes?
Semi-conservative DNA replication
How many origins of replication does a prokaryote have? Eukaryote?
1) single
2) multiple
What is the function of a helicase enzyme?
unwinds DNA
What is the function of a single-stranded binding protein?
prevent strands from reannealing
What is the function of a DNA topoisomerase?
What drug class inhibits DNA gyrase (prokaryotic topoisomerase II)?
- Create a single or double stranded break in the helix to add or remove supercoils
- Fluoroquinolones
What is the function of a primase?
make an RNA primer on which DNA polymerase III can initiate replication
What two polymerases are present in prokaryotes only?
DNA polymerase I and II
In what direction is DNA made?
5’ –> 3’
What is the function of DNA polymerase I?
Degrades RNA primer –> replaces it w/ DNA