BIOCHEMISTRY LAB 4,5,6

Question 1

5 ml ABSOLUTE ALCOHOL

Answer 1

WHITE COAGULUM - ALCOHOL

Question 2

HEATING

Answer 2

WHITE COAGULUM - HEAT

Question 3

1 ml of 10% SILVER NITRATE (AgNO3)

Answer 3

DENSE WHITE PRECIPITATE - HEAVY METALS

Question 4

1 ml of 10% COPPER SULFATE (CuSO4)

Answer 4

BLUE PRECIPITATE - HEAVY METALS

Question 5

HELLER’S REAGENT - 1ml CONCENTRATED NITRIC ACID (HNO3)

Answer 5

WHITE RING - STRONG MINERAL ACIDS

Question 6

ROBERT’S REAGENT - 1ml ROBERT’S REAGENT

Answer 6

WHITE RING - STRONG MINERAL ACIDS

Question 7

1ml 5% PICRIC ACID

Answer 7

YELLOW PRECIPITATE - ALKALOIDAL

Question 8

1ml 5% TANNIC ACID

Answer 8

BROWNISH PRECIPITATE

Question 9

is the sticking of molecules together to form a solid that will come out from the solution.

Answer 9

PRECIPITATION

Question 10

the clumping of particles together in a solution.

Answer 10

COAGULATION

Question 11

refers to the disruption and unfolding of the protein, thus, losing its biological activity.

Answer 11

DENATURATION

Question 12

are generally defined as metals with relatively high densities, atomic weights, or atomic numbers.

Answer 12

HEAVY METALS

Question 13

an acid derived from one or more inorganic compounds. All mineral acids dissociate in water to form hydrogen ions and conjugate bases.

Answer 13

STRONG MINERAL ACIDS

Question 14

are high molecular weight anions.

Answer 14

ALKALOIDAL REAGENTS

Question 15

Standard Amino Acids contain different functional groups within them, which are differentiated in the laboratory using specific chemical tests.

Answer 15

COLOR REACTIONS

Question 16

.5% Copper Sulfate (CuSO4) & 10% Sodium Hydroxide (NaOH)

Answer 16

VIOLET - BIURET TEST

Question 17

0.1 Tirketohydrindene Hydrate solution

Answer 17

DEEP BLUE - NINHYDRIN TEST

Question 18

Nitric Acid (HNO3) & Ammonium Hydroxide (NH4OH)

Answer 18

YELLOW TO ORANGE - XANTHOPROTEIC TEST

Question 19

Glacial Acetic Acid (CH3COOH) & Sulfuric Acid (H2SO4)

Answer 19

VIOLET RING - ADAMKIEWICS TEST

Question 20

40% Sodium Hydroxide (NaOH) & Plumbous Acetate Solution

Answer 20

BLACK - REDUCED SULFUR TEST

Question 21

Naphthol and 95% Ethanol

Answer 21

PURPLE RING - MOLISCH’S TEST

Question 22

AMMONICAL SILVER NITRATE (AgH3N2O) AND AMMONIUM HYDROXIDE (NH4SO4)

Answer 22

WHITE PRECIPITATE - PURINE

Question 23

AMMONIUM HYDROXIDE (NH4OH), 10% NITRIC ACID (HNO3), AMMONIUM MOLYBDATE

AMMONIUM PHOSPHOMOLYBDATE

Answer 23

PURPLE RING - PENTOSE

Question 24

MOLISCH’S REAGENT AND CONCENTRATED SULFURIC ACID (H2SO4)

Answer 24

YELLOW - PHOSPHATE

Question 25

IODINE SOLUTION (I2 AND KI)

Answer 25

DEEP BLUE/BLACK - STARCH

Question 26

BENEDICT’S SOLUTION SODIUM CARBONATE (NaCO3), SODIUM CITRATE (Na3C6H5O7), COPPER SULFATE PETAHYDRATE (CuSO4.5H2O)

Answer 26

YELLOW TO ORANGE - REDUCING SUGAR

Question 27

ETHER

Answer 27

TRANSLUCENT - FATS

Question 28

COPPER SULFATE (CuSO4) AND SODIUM HYDROXIDE (NaOH)

Answer 28

VIOLET - PROTEINS