Biochemistry Cancers

Question 1

What is the nuclear transplant and cloning experiments ?

Answer 1

1) the differentiated cell nucleus can retain totipotency
2) can act as zygote
3) stimulates the production of an entire organism

Question 2

Why we fuse the cells inside the egg with electric pulses?

Answer 2

To mirror the the fertilization process and initiate embryo development

Question 3

We can clone humans

T or F

Answer 3

F only the animal like : mice rats cows pigs goats cats

Question 4

What are the holes that we must achieved in order to produce protein that treats disease?

Answer 4

1)isolation of DNA (cDNA) only interims no Econ’s
2) introduction of the gene in the host cell where it is expressed
3) Growth of the host cell in commercial scale
4j purification of the genes to meet rigorous standers
6) extensive testing to establish clinical efficacy
5) Approval from the regulatory authorities for clinical use .

Question 5

Host cell can. E 
A) Bacteria 
B) yeast 
3) insect 
4) mammalian cells 
All of the above

Answer 5

All of the above

Question 6

T or F

Bacteria and yeast are easier to grow then mammalian cells

Answer 6

T

Because choice depends largely on properties of the protein as well as features of each host cell system

Question 7

How we determine the vector ? Something very important

Answer 7

Depends on how good invade in host cells

Question 8

There are two thing that help medically useful proteins ?

Answer 8

Recombinant DNA and expression vector

Question 9

What is TPA and the important of it ?

Answer 9

Is tissue plasminogen activator, it helps to converts the plasminogen into plasmin that dissolve the clots ( جلطات)
And it is currently produce in the EColi

Question 10

Hoe produce the HGH ?

Answer 10

In the pituitary at the base of the brain

Question 11

H G H is not specific , T or F

Answer 11

F , only the human protein works

Question 12

What is

Creutzfeld -Jacob infection?

Answer 12

Loss of supply from human organs source

Question 13

Synthesis of the viral protein ( HbsAg) failed ……..abs succeed in …….

Answer 13

Bacteria , yeast

Question 14

What are the quality of the DNA vaccines ?

Answer 14

1) are based on plasmids containing protein coding gene
2) have advantages for not being replicative or infectious
3) are cheaper and more staple then other vaccines
4) directly inject or fire DNA coated gold based into muscles

Question 15

What is the name of measles virus ?

Answer 15

Hemagglutinin

Question 16

Alfa 1 anti trypsin in human inhibit,

Answer 16

Elastase , which hydrolyzes connective tissue

Question 17

What is Pompe’s disease ?

Answer 17

( is a glycogen storage disease)
This lead to death at median age of 6 to 8 mounts and I’d diagnosed with the absesns of Alfa glucosidase activity , cardiac hypertrophy , loose of muscles strength

Question 18

1) how to treat the Pompe’s disease ?

Answer 18

By using the transgenic of milk of the rabbit has been successfully used for treating infants

Question 19

In order to step gene expression we synthesis something called (siRNA) which is double strand and it is complementary to the DNA ?

Answer 19

They called this strand as antisense strand it will bind complementary base pair with only that RNA so only affect that protein

Question 20

In order to block the translation of MRNA ?

Answer 20

Using antisense RNA and siRNA

Question 21

What is the signaling molecule for vessel proliferation

Answer 21

Is growth factor

Question 22

RNAI that used in muscles degeneration ( blindness) to stop the profiration of the blood vessels is

Answer 22

Is used to target the mRNA for this growth factor

Question 23

What is humulin and how it is made

Answer 23

It is a synthetic human insulin is produce by genetic engineering ,
It is synthesized by using the recombinant DNA technology .
The r DNA from human insulin is introduced to the E. coli or yeast in order to increase the production

Question 24

Humulin is used to treat what disease?

Answer 24

Diabetes 1 and 2

Question 25

What are the advantages of humulin?

Answer 25

1) absorbed more rapidly than animal derived insulin ( but short act)
2) less expensive then animal derived insulin
3) cause fewer immune and autoimmune reaction

Question 26

Why sport person consider taking HGH?

Answer 26

1) increase the muscles
2) increase the Capacity of oxygen
3) decrease the obesity
4) good for fracture healing
5) stimulate the normal HGH production
6) improve the physical performance

Question 27

Side affects of HGH ?

Answer 27

1) large bone of the abnormalities of HGH
2) pain in the muscles and the joint
3) cause heart disease
4) can cause the growth of cancerous tumor
5) cause edema
6) increase the cholesterol
7) cause numbness and tingling of the skin

Question 28

Children that have deficiency in growth Harmon

Answer 28

Hypo secretion by the pituitary gland cause dwarfism

Question 29

TPA is a medication

T or F

Answer 29

F , it is a part of tremendous it is used after the heart attracted to stop the cloting

Question 30

How the TPA is activated?

Answer 30

It is activated by plasminogen to plasmin which dissolve the clots

Question 31

What is the interferons ?

Answer 31

Are group of proteins produced by viral infected cells and acts as warning system between the infected cells and healthy system in order to activates the immune response

Question 32

Beta interferons are used to treat what ?

Answer 32

It is used to treat multiple sclerosis, that reduced the inflammation that damage the nerves

Question 33

The Antisense sequence is used to inhibit what ?

Answer 33

So Antisense is a sequence that is complementary to the mRNA , it combines to it and prevent if from producing proteins ,
We used it to inhibit the HIV from developing to become AIDS , by artificially making a complementary Antisense sequence and combs in it with the HIV DNA

Question 34

What are the potential disadvantages of vaccine made by traditional methods ?

Answer 34

Replicating the virus

Question 35

What are the advantages of using DNA vaccine ?

Answer 35

Non- replicating virus

Safety, availability , and specificity