Biochemistry Flashcards
1
Q
Noncometitive Inhibition
A
Decreases vmax, same Km
2
Q
Uncompetitive Inhibition
A
Bind to enzyme-substrate complex. Lower both vmax and Km
3
Q
Mixed inhibition
A
Lower vmax. Can bind to either enzyme or ES. If bind more to ES: lower Km. If bind more to enzyme, increase Km.
4
Q
Cell adhesion molecules (CAMs)
A
- Cadherins
- Integrins
- Selectins
5
Q
Cadherins
A
calcium-dependent adhesion, hold similar cell types together
6
Q
Integrins
A
- Have 2-membrane spanning chains (a and b).
- Important in cellular signaling, promote cell division/apoptosis/other process
- Can be used to activate platelets, white blood cell migration, stabilization of epithelium on its basement membrane
7
Q
Selectins
A
Bind to carbohydrate molecules that project from other cell surfaces.
- Expressed on WBC and endothelial cells that line blood vessels
- Role in host defense, including inflammation and WBC migration
8
Q
Isoelectric focusing
A
Basic gel at positive anode, acidic gel at negative cathode
9
Q
Lowry reagent assay
A
Determine level of protein using copper ions getting reduced to tungsten blue
10
Q
a vs. b anomer
A
a: anomeric carbon OH group on opposite side of ring as the CH2OH group on C6.
b: on same side
11
Q
Sucrose, lactose, maltose
A
- Sucrose: glucose-a-1,2-fructose
- Lactose: galactose-b-1,4-glucose
- Maltose: glucose-a-1,4-glucose
12
Q
Vitamin A (carotene)
A
retinal, rentinoic acid
13
Q
Vitamin D (cholecalciferol)
A
calcitriol in kidneys, Ca and P homeostasis in intestines, cause rickets
14
Q
Vitamin E (tocopherols)
A
Biological antioxidants
15
Q
Vitamin K (phylloquinone/menaquinones)
A
important for forming prothrombin
16
Q
Eukaryotic DNA polymerases
A
- DNA polymerase a: synthesize new DNA with polymerase d
- DNA polymerase b: DNA repair
- DNA polymerase gamma: Replicate mitochondrial DNA
- DNA polymerase delta: Synthesize new DNA with polymerase a, fill in gaps after RNA removal with DNA, assisted by PCNA that assemble into trimer for sliding clamp DNA
- polymerase epsilon: DNA repair, assisted by PCNA that assemble into trimer for sliding clamp
17
Q
RNA Polymerase Types
A
- RNA polymerase I: located in nucleolus, synthesizes rRNA
- RNA polymerase II: located in nucleus, synthesizes hnRNA (heterogenous, pre-processed mRNA) and some snRNA
- RNA polymerase III: located in nucleus, synthesizes tRNA and some rRNA
18
Q
Histone acetylation
A
Transcription enhanced
19
Q
Osmotic pressure
A
𝛱 = iMRT
20
Q
Nernst equation
A
E = (61.5/z)(outside ion/inside ion conc)
21
Q
Goldman-Hodgkin-Katz voltage equation
A
Applies nernst equation to find that Vm is related to permeability as well (look at notes)
22
Q
Inner mitochondrial membrane
A
Has high level of cardiolipin, which does not have cholesterol
23
Q
Functions of NADPH
A
- Biosynthesis (mainly FA and cholesterol)
- Assist in bleach production in certain WBC
- Maintain supply of reduced glutathione to protect against reactive oxygen species
24
Q
Glutathione
A
Reducing agent that can help reverse radical formation before damage is done to cell
25
Rate-limiting enzymes for: 1. Glycolysis 2. Fermentation 3. Glycogenesis 4. Glycogenolysis 5. Gluconeogenesis 6. Pentose phosphate pathway
1. Glycolysis: phosphofructokinase-1
2. Fermentation: lactate dehydrogenase
3. Glycogenesis: glycogen synthase
4. Glycogenolysis: glycogen phosphorylase
5. Gluconeogenesis: fructose 1,6-bisphosphatase
6. Pentose phosphate pathway: glucose 6-phosphate dehydrogenase
26
Ways to obtain acetyl-CoA
1. From pyruvate (pyruvate dehydrogenase complex)
2. Beta-oxidation of fatty acids
3. Amino acid catabolism (ketogenic AA)
4. Ketones
5. Alcohol: alcohol dehydrogenase and acetaldehyde dehydrogenase
27
Citric Acid Cycle substrates
Please, Can I Keep Selling Seashells For Money, Officer?
1. Pyruvate
2. Citrate
3. Isocitrate
4. a-Ketoglutarate
5. Succinyl-CoA
6. Succinate
7. Fumarase
8. Malate
9. Oxaloacetate
28
Repair mechanisms, phase of cell cycle, key enzymes repaired
1. Proofreading: S phase, DNA polymerase
2. Mismatch repair: G2, MSH/MLH1 in euk or MutS/MutL in prok
3. Nucleotide excision repair: G1, G2, Excision endonuclease
4. Base excision repair: G1, G2, glycosylase, AP endonuclease
29
Blot types
1. Southern blot: presence and quantity of various DNA strands in a sample. Restriction enzymes, palindromes
2. Northern blot: detect RNA
3. Western blot: detect protein levels
4. Eastern blot: post-translational modifications on proteins
5. Far eastern blot: lipids, from high-performance TLC
30
GLUT 2 vs. GLUT4
1. GLUT2: low-affinity transporter in hepatocytes and pancreatic cells. High Km, pick up glucose proportional to its blood concentration. Not responsive to insulin, but serve as glucose sensor for insulin release.
2. GLUT4: high-affinity transporter in adipose tissues and muscle. Km similar to normal [glucose] in blood. Responsive to insulin, increase intake of glucose by increasing number of GLUT4 receptors.
31
Absorption of digested lipids
1. Short-chain fatty acids: diffuse to blood
2. Bile salts: active transport to blood
3. Packaged into chylomicrons, that leave intestine as lacteals and re-enter bloodstream via thoracic duct
32
How insulin affects fatty acid mobilization
Fall in insulin levels activate hormone-sensitive lipase (HSL) that hydrolyzes triaglycerols. HSL effective within adipose cells, but need lipoprotein lipase (LPL) for metabolism of chylomicrons and VLDL.
33
Lipoprotein definition
Aggregates of apolipoproteins and lipids
34
Lipoproteins and their functions
1. Chylomicrons: transport dietary triaglycerols, cholesterol, and cholesteryl esters from intestine to tissues
2. VLDL: transports triaglycerols and fatty acids from liver to tissues
3. IDL (VLDL remnants): picks up cholesteryl esters from HDL to become LDL. Picked up by liver
4. LDL: delivers cholesterol into cells
5. HDL: picks up cholesterol accumulating in blood vessels. Delivers cholesterol to liver and steroidogenic tissues. Transfers apolipoproteins to other lipoproteins.
35
Where is VLDL produced and assembled?
Liver cells
36
Apoproteins
Also called Apolipoproteins. Protein component of lipoproteins. Receptor molecules, involved in signaling leading to diverse purposes.
37
Cholesterol sources
Mostly from LDL or HDL, but de novo synthesis in liver
38
Cholesterol de novo synthesis
In the liver, driven by acetyl-CoA and ATP.
| Rate-limiting step: synthesis of mevalonic acid in SER, catalyzed by HMG CoA reductase.
39
Cholesterol synthesis regulation
1. Increased levels of cholesterol inhibit
2. Increased insulin promotes
3. Depends on regulation of HMG-CoA reductase gene expression in cell
40
Lecithin-cholesterol acyltransferase (LCAT)
Enzyme in bloodstream, activated by HDL apoproteins. Adds fatty acid to cholesterol, producing soluble cholesterol esters as in HDL.
41
Cholesteryl ester transfer protein (CETP)
Facilitates transfer of cholesteryl esters from HDL to other lipoproteins such as IDL (which becomes LDL after this transfer)
42
Essential fatty acids
a-linolenic acid, linolenic acid (important for cell membrane fluidity)
43
Ketogenic amino acids
Leucine, lysine, isoleucine, phenylalanine, threonine, tryptophan, tyrosine
44
Glucogenic amino acids
All except for leucine and lysine
45
Tissues in which glucose uptake is not affected by insulin
1. Nervous tissue
2. Kidney tubules
3. Intestinal mucosa
4. Red blood cells
5. b-cells of pancreas
46
How plasma glucose levels affect insulin levels
Increased plasma glucose = higher ATP. Increased ATP lead to higher calcium release in cell, promoting exocytosis of preformed insulin from intracellular vesicles
47
What promotes secretion of glucagon
1. Low plasma glucose levels
| 2. Amino acids (especially basic ones)
48
T3 and T4
T3: rapid increase in metabolic rate
T4: slow increase in metabolic rate
1. Accelerate cholesterol clearance
2. Increase glucose absorption from small intestine
3. Epinephrine requires T3 and T4 for function
49
Preferred fuels in well-fed and fasting states in 1. Liver 2. Resting skeletal muscle 3. Cardiac muscle 4. Adipose tissue 5. Brain 6. RBC
1. Liver: well-fed = glucose and AA, fasting = FA
2. Resting skeletal muscle: well-fed = glucose, fasting = FA, ketones
3. Cardiac muscle: same as resting skeletal
4. Adipose tissue: well-fed = glucose, fasting = FA
5. Brain: both glucose, but ketones in prolonged fast
6. RBC: both glucose
50
Respiratory quotient
RQ=CO2 produced/O2 consumed
51
Orexin
Further increases appetite
52
BMI
mass/(height^2)
