Biochemistry Flashcards
Chronic alcoholism will lead to
Thiamine deficiency (acquired pyruvate dehydrogenase deficiency) - leading to fatal pyruvic and lactic acidosis
Final common pathway for aerobic oxidation of all nutrients: carbohydrates, protein and lipids
Citric acid cycle/ krebs cycle/ TCA
TCA occurs at the mitochondrial matrix of all mitochondrial containing cells except which part
Succinate dehydrogenase
- occurs within inner mitochondrial membrane
What is the rate limiting step in the TCA?
Enzyme?
Isocitrate –> a-ketoglutarate
Enzyme: isocitrate dehydrogenase
Wernicke-korsakoff syndrome results from
Thiamine deficiency
- caused by chronic alcoholism
Leigh Syndrome
Subacute necrotizing encephalomyelopathy
-Defects in mitochondrial ATP production
Most common defect in glycolysis
Pyruvate kinase deficiency
Major cause of intravascular hemolytic anemia
G6PD deficiency
Present with Heinz bodies
Secondary cause: pyruvate kinase deficiency
Most common biochemical cause of congenital lactic acidosis?
Pyruvate dehydrogenase deficiency
-X-linked dominant
How many ATP is generated from aerobic glycolysis
6 if aerobic glycolysis is at skeletal muscle and brain (1NADH=2atp
8 if aerobic glycolysis is at liver, kidney and heart (1NADH= 3 atp
How many ATP is generated from anaerobic glycolysis
2
Where does anaerobic glycolysis occur?
Lens, cornea, RBC, WBC, renal medulla, testes
Products of TCA in 1 acetyl coa
2 CO2
3 NADH
1 FADH2
1 GTP
How many ATP produced per glucose that enters TCA
In liver, pancreas and heart
8 from glycolysis ( 6 in muscles and brain)
6 from conversion of pyruvate to acetyl coa (2 pyruvates converted
24 from acetyl coa in TCA (18 in muscles and brain
38 total per glucose ( 28 in muscles and brain)
