Biochemistry Flashcards
What are the products of glycolysis?
2 ATP, 2 NADH, 2 Pyruvate
What is the rate limiting enzyme in glycolysis?
Phosphofructokinase-1
Where in a cell does glycolysis take place?
Cytoplasm
Where in a cell does the Krebs cycle take place?
Mitochondrial matrix
Name 4 ways in which ATP can be produced?
- Krebs cycle.
- Glycolysis.
- Oxidative phosphorylation.
- Substrate level phosphorylation.
What enzyme of glycolysis is inhibited in acidosis?
Phosphofructosekinase-1 (PFK-1 is pH dependent).
What enzyme catalyses the formation of 3-phosphoglycerate from 1,3-bisphosphoglycerate?
Phosphoglyceratekinase.
What enzyme catalyses the formation of glyceraldehyde-3-phosphate from dihydroxyacetone phosphate?
Triose phosphate isomerase.
Oxidative phosphorylation: What enzyme transports protons into the mitochondrial matrix?
ATP synthase
Is the mitochondrial membrane permeable or impermeable to ions?
Impermeable
What are steroid hormones synthesised from?
Cholesterol
Name 2 electron accepting coenzymes.
- NAD
- FAD
Approximately how many ATP molecules are produced from oxidation of NADH?
2.5
Approximately how many ATP molecules are produced from oxidation of FADH2?
1.5
Briefly describe what happens in anaerobic respiration.
NAD is regenerated from NADH. Pyruvate, from glycolysis, forms lactate. Lactate dehydrogenase catalyses this reaction. NAD goes back to glycolysis so ATP can be produced.