Biochemistry Flashcards

1
Q

What are the products of glycolysis?

A

2 ATP, 2 NADH, 2 Pyruvate

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2
Q

What is the rate limiting enzyme in glycolysis?

A

Phosphofructokinase-1

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3
Q

Where in a cell does glycolysis take place?

A

Cytoplasm

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4
Q

Where in a cell does the Krebs cycle take place?

A

Mitochondrial matrix

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5
Q

Name 4 ways in which ATP can be produced?

A
  • Krebs cycle.
  • Glycolysis.
  • Oxidative phosphorylation.
  • Substrate level phosphorylation.
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6
Q

What enzyme of glycolysis is inhibited in acidosis?

A

Phosphofructosekinase-1 (PFK-1 is pH dependent).

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7
Q

What enzyme catalyses the formation of 3-phosphoglycerate from 1,3-bisphosphoglycerate?

A

Phosphoglyceratekinase.

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8
Q

What enzyme catalyses the formation of glyceraldehyde-3-phosphate from dihydroxyacetone phosphate?

A

Triose phosphate isomerase.

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9
Q

Oxidative phosphorylation: What enzyme transports protons into the mitochondrial matrix?

A

ATP synthase

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10
Q

Is the mitochondrial membrane permeable or impermeable to ions?

A

Impermeable

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11
Q

What are steroid hormones synthesised from?

A

Cholesterol

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12
Q

Name 2 electron accepting coenzymes.

A
  1. NAD
  2. FAD
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13
Q

Approximately how many ATP molecules are produced from oxidation of NADH?

A

2.5

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14
Q

Approximately how many ATP molecules are produced from oxidation of FADH2?

A

1.5

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15
Q

Briefly describe what happens in anaerobic respiration.

A

NAD is regenerated from NADH. Pyruvate, from glycolysis, forms lactate. Lactate dehydrogenase catalyses this reaction. NAD goes back to glycolysis so ATP can be produced.

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16
Q

What enzymes regulate the Krebs cycle?

A
  1. Pyruvate dehydrogenase.
  2. Citrate synthase.
  3. Isocitrate dehydrogenase.
  4. α-ketoglutarate dehydrogenase.
17
Q

Where does oxidative phosphorylation occur?

A

Mitrochondrial matrix

18
Q

What is the final electron acceptor?

A

Oxygen

19
Q

Describe the move of electrons in the electron transport chain?

A

Components of ETC accept electrons (reduced) and pass them along (oxidised)

20
Q

What is normal blood glucose?

A

4.5 - 5.5 mmol/L

21
Q

How is blood glucose maintained?

A

Through the action of anabolic and catabolic hormones.

22
Q

What is the fate of pyruvate in anaerobic conditions?

A

Lactate formation catalysed by lactate dehydrogenase

RegenerationonNAD+

23
Q

What regulates PFK?

A

ATP (inhibitor)
AMP (activator)
Citrate (inhibitor)
Fructose 2, 6 biphosphate (activator)