bio task 3 state rankers Flashcards
Define PCR
Polymerase Chain Reaction - a technique used to amplify a specific DNA sequence, allowing for the production of many copies of that sequence.
Define DNA polymerase
An enzyme that can replicate DNA by catalyzing the synthesis of new DNA strands complementary to a template strand.
Define DNA template
The original DNA strand that serves as a model for the synthesis of new DNA strands during replication.
Define primer
Short, single-stranded DNA sequences that serve as starting points for DNA synthesis during PCR.
Define denaturation
The process of separating the double-stranded DNA molecule into single strands, typically done by heating the sample.
Define annealing
The step in PCR where the primers bind to their complementary sequences on the DNA template.
Define extension
The step in PCR where the DNA polymerase synthesizes new DNA strands complementary to the template.
Define cycle
One round of the three main steps in PCR: denaturation, annealing, and extension.
Define mutation
A change in the genetic material of a cell – the sequence of nucleotides is altered.
Define mutagen
An environmental agent that alters DNA and causes mutations.
Define mutagenesis
The process of inducing a mutation.
Define induced mutation
A mutation that results from exposure to a mutagen.
Define carcinogenic
Cancer-causing.
Define alkylating agent
A chemical mutagen that adds methyl and ethyl groups to different parts of DNA nucleotides.
Define base analogue
A chemical mutagen that is similar in structure to the nitrogenous bases of DNA and can be mistakenly integrated into DNA during replication.
Define intercalating agent
A chemical mutagen that inserts itself between DNA bases, causing a code frameshift.
Define non-biological mutagen
A naturally occurring mutagen that is not biological in origin, such as metals.
Define biological mutagen
A naturally occurring mutagen that is biological in origin, such as viruses, bacteria, and their products.
Define transposon
Sections of DNA that can spontaneously fragment and relocate or multiply within the genome.
Define ionizing radiation
Radiation that has sufficient energy to detach electrons from atoms and thereby ionize them, breaking chemical bonds in molecules including DNA.
Define non-ionizing radiation
Radiation that does not have enough energy to ionize atoms, such as UV, visible light, and radio waves.
Define pyrimidine dimer
Adjacent pairs of thymine or cytosine bases on the same DNA strand that become attached to each other due to UV radiation.
Define point mutation
A single nucleotide variation in the DNA sequence.
Define frameshift mutation
A mutation involving the insertion or deletion of a nucleotide pair, shifting the reading frame of the DNA.
Define nonsense mutation
A point mutation that creates a premature stop codon, resulting in a shortened polypeptide.
Define missense mutation
A point mutation that results in the coding of a different amino acid.
Define silent mutation
A point mutation that does not change the amino acid sequence of the resulting protein.
Define neutral mutation
A point mutation that results in a change in amino acid, but the new amino acid is the same type as the original.
Define chromosomal deletion
A mutation where a section of DNA is removed and not replaced.
Define chromosomal insertion
A mutation where a portion of DNA is duplicated and inserted into a chromosome.
Define chromosomal inversion
A mutation where a section of DNA is removed, turned around 180 degrees, and reinserted.
Define chromosomal translocation
A mutation where a section of DNA is moved from one chromosome to another non-homologous chromosome.
Define aneuploidy
A change in the normal chromosome number, either an extra copy or a missing chromosome.