Bio Lab Exam 2 Flashcards

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1
Q

1

A

1) FALSE

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2
Q

2

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2) TRUE

The concentration of a solution is commonly
listed as molarity.

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3
Q

3

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3) FALSE

The positive control is a known substance
that is expected to be positive when the test
reagent is added. It is used to be sure the test
solution is working properly.

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4
Q

4

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4) FALSE

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5
Q

5

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5) FALSE

Since lipids are nonpolar, they dissolve in nonpolar solvents.

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6
Q

6

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6) TRUE

Phenolphthalein is used as a pH indicator
because it turns red in basic conditions.

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7
Q

7

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7) FALSE

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8
Q

8

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8) TRUE

Recall that the Fluid Mosaic Model uses a
phospholipid bilayer as its foundation. In
this bilayer, the polar phospholipid heads
face out, and the nonpolar phospholipid tails
avoid water by remaining in the interior of
the membrane. The membrane interior is
nonpolar, so nonpolar molecules move
across readily.

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9
Q

9

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9) FALSE

Water will flow from an area of high water
potential to an area of low potential.

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10
Q

10

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10) [A, B]

Stock solutions only have to be diluted, not
made from scratch each time they are
required.

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11
Q

11

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11) [A, D]

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12
Q

12

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12) [A, B]

Carbohydrates are the main ingredient in
soda and candy.

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13
Q

13

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13) [D]

Steak is the only choice with the majority of
its calories from protein.

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14
Q

14

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14) [C]

Butter is mainly saturated fat.

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15
Q

15

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15) [B, C]

DNA exclusively can be detected using Dische diphenylamine and has thymine as a nitrogenous base.
Both DNA and RNA have cytosine as a
nitrogenous base. Ribose has an group at C2, while DNA has H in that position.

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16
Q

16

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16) [A, B, C, D]

The speed at which diffusion occurs is
influenced by the size, polarity, solubility,
and especially by the concentration gradient
of molecules.

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17
Q

17

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17) [B, C]

Dialysis tubing has pores of a certain size,
consequently, the size of a molecule is a
determining factor in whether the molecule
will be able to pass through dialysis tubing.
Larger molecules may be too large to diffuse
through the tubing.
A great difference between the
concentration of a substance outside of the
dialysis tubing compared to its concentration
inside the dialysis tubing is known as a steep
concentration gradient. The steeper the
concentration gradient, the faster diffusion
will occur.

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18
Q

18

A

18) [A, B, D]

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19
Q

19

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19) [A, B, C, E]

The fluid mosaic model of cell membrane
structure has a phospholipid bilayer as a
foundational layer. Phospholipids are
arranged with their heads pointing out and
their tails facing each other in the interior of
the membrane. Interspersed amongst these
phospholipids are integral proteins, which
go all the way through the bilayer.
Peripheral proteins occur along the side of
the membrane, but do not penetrate the
phospholipid bilayer. Carbohydrates extend
in chains from the outside of the cell
membrane.

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20
Q

20

A

20) [A, B, C, D]

All of these would be possible if human
tissue could maintain viability after freezing.
All of these would be possible if human
tissue could maintain viability after freezing.

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21
Q

21

A

21) B

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22
Q

22

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22) B

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23
Q

23

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23) A

By definition, the number of grams per 100
ml refers directly to the percent of solute in
that solution.

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24
Q

24

A

24) E

There are always 12 items in a dozen.

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25
Q

25

A

25) B

The number of molecules in a mole is
similar to the idea of a dozen. One dozen
elephants indicates 12 individuals, and one
dozen mice indicates 12 individuals. It does
not matter that elephants are larger than
mice; the concept relates the number of
individuals only, which is 12 per dozen.
One mole of a substance always indicates
molecules. One mole of
water weighs 18g, while one mole of
glucose weighs 180g. One mole of water has
the same number of molecules as does a
mole of glucose, because the number of
molecules per mole does not change. A mole
indicates the number of individual
molecules present, just as a dozen does.

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26
Q

26

A

26) B

One atom of Mg weighs 24
One atom of S weighs 32
One atom of O weighs 16
Weight of = 24 + 32 + 4(16)

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27
Q

27

A

27) C

The molecular weight of is 120
g/mole. 120 g/mole (0.5 moles/L) = 60 g/L

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28
Q

28

A

28) D

A stock solution is a concentrate that can be
diluted when needed.

29
Q

29

A

29) C

The molecular weight of glucose is 180 g.
This comes from 6 carbon atoms with
atomic weights of 12 each, 12 hydrogen
atoms with atomic weights of 1 each, and 6
oxygen atoms with atomic weights of 16
each.
from carbon
from hydrogen
from oxygen
A one molar solution means one mole in
one liter of water, so a 0.3M solution would
use 0.3 moles.
180 (0.3) = 54

30
Q

30

A

30) D

162 g / 180 grams per mole = 0.90M

31
Q

31

A

31) C

The most efficient antacid works by
absorbing more stomach acid.

32
Q

32

A

32) B

Bases take up , so a buffer would
counteract this by donating.

33
Q

33

A

33) A

Bicarbonate is a buffer of human blood and
other solutions.

34
Q

34

A

34) B

Strong acids have high concentrations. A
buffer would neutralize this by picking up.

35
Q

35

A

35) D

Each increment of the pH scale
corresponds to a power of 10. A difference of 2 pH units is a difference of . A solution with a pH value of 4 is a weaker
acid, so it would be , or 100 times
weaker than a solution with a pH value of 2.

36
Q

36

A

36) E

37
Q

37

A

37) A

38
Q

38

A

38) B

39
Q

39

A

39) B

The only positive test results in the example
above are the Biuret test. A violet result
indicates peptide bond, and therefore protein
is present.

40
Q

40

A

40) A

Lipids have many C-H bonds and little
oxygen in their fatty acid components.

41
Q

41

A

41) A

Carbohydrates are characterized by a 1:2:1
ratio of C:H:O.

42
Q

42

A

42) D

43
Q

43

A

43) C

Iodine turns blue-black in the presence of
starch.

44
Q

44

A

44) D

Diffusion is the movement of material from
high concentration to low concentration.
Osmosis is a specific type of diffusion.
Osmosis specifically refers to the flow of a
solvent (usually water) from high to low
concentration.

45
Q

45

A

45) B

Tonicity refers to solute concentration, but
water concentration can be inferred from
tonicity. The cell is hypertonic because it
has more solute (20%) than the solution it is
in (5%).

46
Q

46

A

46) B

Since the solution in the beaker is 95%
water, and the solution in the cell is 80%
water, water will flow into the cell and the
cell will swell.

47
Q

47

A

47) C

Water is the solvent in this example, and
osmosis is the movement of a solvent from
high to low concentration.

48
Q

48

A

48) B

For our purposes in a lab, we can visualize
the solute and the water in a beaker make up
100% of what is contained in the beaker. If
we increase the amount of solute, the
proportion of water would decrease. Solute
concentration and water potential are
inversely proportional. As solute increases,
water potential decreases.

49
Q

49

A

49) A

Water flows from high water potential to
low water potential.

50
Q

50

A

50) A

Tonicity refers to solute concentration, but
water concentration can be inferred from
tonicity. Solutes will not move through these
membranes, but water will move through
and will cause a change in the appearance of
the cell. In a hypertonic environment, there
is more solute and less water. Water will
move from a higher concentration inside of
the cell to a lower concentration outside of
the cell, causing the cell to shrink.

51
Q

51

A

51) B

As molecular weight increases, the rate of
diffusion decreases. Since substance #4 has
the lowest molecular weight, it will move
faster than the other substances.

52
Q

52

A

52) C

The 20% NaCl solution is hypertonic to the
cell, so the cell will shrink. The cell wall is
not affected by the salt solution, so it will
remain in place while the cell shrinks within
it.

53
Q

53

A

53) A

Heat is the cause of the random pattern of
the Brownian movement.

54
Q

54

A

54) A

Tonicity refers to solute concentration, but
water concentration can be inferred from
tonicity. Hypertonic solutions cause cells to
shrink because there is proportionally less
water (and more solute) present in the
environment than there is inside the cell.
Through osmosis, the water would move
from high to low concentration. Due to the
cell wall, a plant cell would shrink, but a
decrease in size would occur inside of the
strong cell wall.

55
Q

55

A

55) A

Diffusion is the movement of material from
high to low concentration. This will occur
until a uniform distribution of the material is
present.

56
Q

56

A

56) C

57
Q

57

A

57) C

Amphipathic refers to the possession of
hydrophobic and hydrophilic regions, as
exemplified by integral proteins.

58
Q

58

A

58) C

Betacyanin, a red pigment, is housed in the
central vacuole of beet cells. If this pigment
is leaking, it means the cell membrane has
been stressed to allow its release.

59
Q

59

A

59) A

Recall that the composition of a cell is
mainly water, so what happens to the water
will have the greatest impact in this instance.
When water freezes, it expands. Inside a
cell, water expansion due to freezing would
rupture the cell membrane, which if
widespread would destroy tissue and cause
frostbite.

60
Q

60

A

60) D

Since methanol and acetone are nonpolar,
they would act on the nonpolar fatty acid
tails of the phospholipids.

61
Q

61

A

61) A

Small, nonpolar molecules tend to pass
rapidly through the cell membrane because
of the nonpolar nature of the membrane
interior. A polar molecule would be repelled
by the nonpolar interior.

62
Q

62

A

62) distilled water

The negative control is not expected to react
with the test reagent. If it does have a
positive reaction, there is contamination or a
problem with the test reagent.

63
Q

63

A

63) peptide

Peptide bonds form between amino acids in
a protein.

64
Q

64

A

64) permeable

Permeability refers to the possibility that
materials will move through a membrane. If
a membrane allows some materials through
but not others, it is called semi-permeable,
or differentially permeable.

65
Q

65

A

65) dialysis

Just as the cell membrane is differentially
permeable, dialysis tubing is differentially
permeable. That shared property makes
dialysis tubing a good model for a cell
membrane.

66
Q

66

A

66) kinetic

Kinetic energy is the energy of movement.

67
Q

67

A

67) tonoplast

The tonoplast is a membrane that surrounds
the central vacuole. The tonoplast provides
channels through which materials can pass.

68
Q

68

A

68) absorbance

An increase in pigment is read by the
spectrophotometer as increased absorbance.

69
Q

69

A

69) nonpolar

Both acetone and methanol are nonpolar