Bio lab Flashcards
Introduction
Subject under study, context for work reported, & hypothesis under investigation (including variables)
Materials and Methods
Explains how study is carried out & explain each control
Results
describes data trends
Discussion
explain and interpret results in light of what’s being studied
Format of lab report
Times New Roman, 12-pt font, double spaced, 1-inch margins
Honor Code
I pledge that I have given nor received unauthorized assistance on this assignment and it is entirely my own creative work
Objective vs. Subjective
Objective observations can be verified, subjective observations are observer specific
Hypothesis
How independent variable will effect dependent variable. Must be testable
Occam’s Razor
if there are several explanations that might fit an observation, the simplest is most probable & therefore best
Agar
polysaccharide compound found in cell walls of red algae. Causes medium to stay in place
Prokaryotes
Unicellular; lack membrane bound organelles
Reproduction: mainly asexual
Feeding: absorption
Bacteria
Nitrogen fixation: convert nitrogen from atmosphere into usable forms
Decomposers
2 types: Photosynthetic & chemsynthetic
Chemosynthetic
bacteria obtain their energy through oxidation of inorganic substances
Bacilli
rod-shaped
Cocci
small spheres
Spirilla
cork-screw shaped
Blue-green algae or Cyanobacteria
possess chlorophyll a
Types of cyanobacteria (blue-green algae)
Unicellular: gloeocapsa
Colonial: oscillatoria
Fungi
eukaryotic, heterotrophic & generally multicellular
Staphylococcus
2-3 mm wide colonies, opaque, off-white sometimes yellow. Gram-positive (purple) will be seen in bunches [think “staph” infection
Streptococcus
Forms tiny colonies less than 1 mm, off-white transparent. Gram positive. [think “strept” throat
Bacillus
Forms large colonies over 3mm, usually off-white, opaque, waxy-looking. Gram-positve
Coliforms
2-3 mm wide, transparent off white, foul smelling, wet-looking. Gram-negative rods. Common in intestines [think E.coli]
pseudomonas
yellow-green, green, or blue-green pigment diffuses onto medium
Yeasts
large colonies 4 mm wide, white & glistening & raised, large oval cells
Molds
characteristically hairy-looking. Stains show hyphae (broken ends and size distinguish them from bacilli
Gram stain
positive= thick cell wall negative= thin cell wall
Protists kingdom
contains many eukaryotic single celled and colonial organisms. Most artificial of all kingdoms
Protist: Paramecium
single celled organism belonging to the phylum Ciliophora. Have hair-like cilia
oral groove
fluids and food particles are forced here by movement of the paramecium’s cilia
cytopharnyx
lower end of oral groove where food particles accumulate
food vacuole
envelops food particles. Digestive enzymes from lysosomes enter and digest food
Anal pore
where food vacuole migrates, attaches then empties
contractile vacuoles
remove excess fluids from cell (paramecium has 1 at each end)