Bio Lab Flashcards
Alternate metabolic pathway (who) and (when) ?
Fungi, when oxygen isn’t present in anaerobic environment
Anaerobic
Process of cellular energy production without use of oxygen
Organic final electron acceptor is….
Oxygen
Energy yield from glycolysis stage only(how much)
2 ATP
What is produced during alcohol fermentation?
CO2 and ethanol
Substrate
(Reactants) sugars
Different conditions that make alcohol fermentation more optimal
Effect of diastase?
Helps digest starch and break down glucose
Stroma
Dense fluid surrounding the grana in the chloroplast
Thylakoid
Sacs arranged into grana
Chloroplast
Organelles which photosynthesis occurs
Grana
Group of stacks
Stomata
Small openings that allow gas exchange to occur
Epidermis
Waterproofing the leaf
Mesophyll
Location of photosynthetic cells
Cuticle
Protects plants to prevent drying out
Granum
Site for light reaction of photosynthesis
Photosynthesis
Process that coverts solar energy into chemical energy
Autotrophs
Include plants, algae, and some prokaryotes
Heterotrophs
Consumers of biosphere (animals, fungi, bacteria) they obtain organic material from other organisms
3 reasons cells divide
Reproduce
Growth
Replace
2 main stages of cycle
Interphase (non dividing)
Mitosis (dividing)
3 subphases of interphase
G1 phase: growth
S phase: synthesis of DNA
G2 phase: prep for cell division
4 subphases of mitosis
Prophase: condense chromatin, attach fibers, breakdown of nuclear envelope
Metaphase: alignment
Anaphase: separation
Telophase: division
Interphase
Prior to mitosis
Prophase
Chromatin condenses to chromosomes, nuclear envelope and nucleolus disappear, spindle fibers form
Metaphase
Chromosomes line up in the middle
Anaphase
Sister chromatids pull apart
Telophase
Reorganization phase ( undo the events of prophase by:
-Chromosomes relax into chromatin
-Nuclear envelope and nucleolus reappear
-Spindle fibers break down
Monomer of DNA as :
Nucleotide
Phosphate
Bonded to pentos deoxyribose through covalent bonds
Nitrogen base
Bonded to pentos deoxyribose through hydrogen bonds
4 nitrogen bases
Adenine, guanine (purines)
Thymine, cytosine (pyrimidines)
Reagents used for DNA extraction
Detergent, ethanol, salt, meat tenderizer
Ethanol
Precipitates dna
Salt
Stabilizes the DNA
Meat tenderizer
Breaks down histone protein
Detergent
Opens membrane to get the DNA
Histone protein
Provides structural support for a chromosome
DNA has what kind of charge
Negative
PCR (polymerase chain reaction)
Can be used to amplify the amount of DNA
What percentage of human DNA contains specific instructions for making proteins which are called…
1-5%
Genes
Tandem repeats
two or more DNA bases that is repeated numerous times in a head to tail manner on chromosomes
Restriction enzyme
(Bacterial origin) Cuts DNA
How many bands are visible
2 bands
Helicase
Breaks the weak hydrogen bonds holding the DNA strands together
DNA polymerase
Catalyze the addition of the new nucleotides during replication
DNA ligase
Facilitates joining of DNA strands together by catalyzing the formation of bond
Leading strand
Forms towards dividing part
Lagging strand
Forms away
Okazaki fragment
Pieces of lagging strand joined together by enzyme ligase
Semi conservative replication
Each resulting daughter molecule consists of 1 original strand and 1 newly synthesized strand