Bio 141 Exam 3 Flashcards
What was Crick’s adapter molecule and how was it discovered?
The adapter molecule is tRNA and it was discovered by accident by biologists.
What is an “anticodon”?
An anticodon is a set of 3 ribonucleotides that forms base pairs with the mRNA codon
How many different codons are there?
61
How many different anticodons are there?
40
Why is there a difference between number of codons and anticodons?
Wobble hypothesis- nonstandard base pairing is acceptable in third position of codon as long as it doesn’t change the amino acid that the codon specifies
There must be at least how many different “aminoacyl tRNA synthetases?”
20 to account for each of the 20 major amino acids
What do aminoacyl tRNA synthetases do?
These enzymes catalyze the addition of amino acids to tRNA.
What are the three phases of protein synthesis (translation)?
initiation, elongation, and termination
Initiation in translation?
a small ribosomal subunit binds to the mRNA ribosome binding site. In eukaryotes, initiation factors bind to the 5’ cap on mRNAs and guide it to the ribosome.The initiator aminoacyl tRNA bearing f-met binds to the start codon, then to finish off initiation a large ribosomal subunit binds
Elongation in translation?
new tRNA moves into A site where its anticodon base pairs with the mRNA codon, then a peptide bond forms between amino acid attached to the tRNA in P site and A site, the tRNA attached to polypeptide chain moves into the P site, while the A site is now empty
Termination in translation?
translocation opens the A site and exposes one of the stop codons, a release factor then fills the A site, a reaction that frees the polypeptide
How do mRNAs and ribosomes get together to start the process of translation?
A section of ribosomal RNA in a small ribosomal subunit bins to a complementary sequence on an mRNA, also known as the ribosome binding site. It is six nucleotides upstream from the AUG start codon.
What is a “ribozyme” and how do we know that a ribozyme catalyzes peptide bond formation?
A ribozyme is RNA that catalyzes protein synthesis. We know that a ribozyme catalyzes peptide bond because it is known that the active site in the ribosome catalyzes this process. Also, it was discovered that this active site is composed of rRNA.
How does protein synthesis conclude when the ribosome reaches the end of the message?
It concludes when translocation exposes the stop codon in an mRNA strand. A release factor then enters the A site and breaks the bond between the P-site tRNA and the polypeptide, releasing the polypeptide.
What processes take place after the translation of mRNA into a linear sequence of amino acids (i.e., into a polypeptide or protein) has been completed?
protein folding, some proteins receive sorting signals, some gain sugar/lipid groups crucial for normal functioning, many are altered by enzymes that add or remove phosphate groups