Bio 110 Chapter 4 Flashcards
cells are the simplest collection of
matter that can live.
cells were first observed by
Robert Hooke in 1665 in cork.
Antoni van Leeuwenhoek
worked with more refined lenses and later described blood, sperm, and organisms in pond water.
The cell theory states
all living things are composed of cells, and all cells come from other cells.
Cell size must be large enough to
house DNA, proteins and structures needed to survive, but small enough to allow for a surface to volume ratio that will allow adequate exchange with the environment.
light microscope (LM)
most commonly used
light passes through a specimen, then through glass lenses, and then to the viewer’s eye.
With a light microscope specimens can be magnified up to
1,000 times the actual size of the specimen.
magnification
to increase the apparent size of an object
resolution
a measure of the clarity of an image. (the ability to show 2 close objects as separate)
since the human eye and the microscope have limits on resolution (the ability to distinguish between small objects),
light microscopes cannot provide the details of a small cell’s structure.
using light microscopes, scientists studied
microorganisms, animal and plant cells, and some structures within the cells.
In the 1950’s scientists started using a powerful microscope to view the ultrastructure of cells called the
Electron microscope (EM)
Electron microscopes can
resolve biological structures as small as 2nm
with an EM specimens can be magnified up to
100,000 times.`
most cells are between
1 and 100 micrometers (um) in diameter.
LMs can’t resolve detail finer than
about 0.2 micrometers (um)
Instead of light EM uses
a beam of electrons.
the EM has a
100-fold improvement over the light microscope in resolution.
Scanning electron microscopes (SEM) study
the detailed architecture of cell surfaces.
Using SEM the sample is
coated in gold and an electron beam excites surface electrons, and these electrons are detected by a device that translates their pattern to a video screen.
Transmission Electron microscopes (TEM) study
the details of internal cell structure.
The TEM aims
electron beam through a thin section of the specimen with is stained with heavy metal atoms, binding more in some places than others.
TEM lenses are
electromagnets.
Electron microscopes can’t be used to study
living specimens, because the ways to prepare the samples kill the cells.