Bio 110 Chapter 4 Flashcards
cells are the simplest collection of
matter that can live.
cells were first observed by
Robert Hooke in 1665 in cork.
Antoni van Leeuwenhoek
worked with more refined lenses and later described blood, sperm, and organisms in pond water.
The cell theory states
all living things are composed of cells, and all cells come from other cells.
Cell size must be large enough to
house DNA, proteins and structures needed to survive, but small enough to allow for a surface to volume ratio that will allow adequate exchange with the environment.
light microscope (LM)
most commonly used
light passes through a specimen, then through glass lenses, and then to the viewer’s eye.
With a light microscope specimens can be magnified up to
1,000 times the actual size of the specimen.
magnification
to increase the apparent size of an object
resolution
a measure of the clarity of an image. (the ability to show 2 close objects as separate)
since the human eye and the microscope have limits on resolution (the ability to distinguish between small objects),
light microscopes cannot provide the details of a small cell’s structure.
using light microscopes, scientists studied
microorganisms, animal and plant cells, and some structures within the cells.
In the 1950’s scientists started using a powerful microscope to view the ultrastructure of cells called the
Electron microscope (EM)
Electron microscopes can
resolve biological structures as small as 2nm
with an EM specimens can be magnified up to
100,000 times.`
most cells are between
1 and 100 micrometers (um) in diameter.
LMs can’t resolve detail finer than
about 0.2 micrometers (um)
Instead of light EM uses
a beam of electrons.
the EM has a
100-fold improvement over the light microscope in resolution.
Scanning electron microscopes (SEM) study
the detailed architecture of cell surfaces.
Using SEM the sample is
coated in gold and an electron beam excites surface electrons, and these electrons are detected by a device that translates their pattern to a video screen.
Transmission Electron microscopes (TEM) study
the details of internal cell structure.
The TEM aims
electron beam through a thin section of the specimen with is stained with heavy metal atoms, binding more in some places than others.
TEM lenses are
electromagnets.
Electron microscopes can’t be used to study
living specimens, because the ways to prepare the samples kill the cells.
Differential interference light microscopes
amplify differences in density so that structure of living cells appear almost 3D
fluorescent microscopy
a type of light microscopy.
Bacteria and archaea are
prokaryotic cells
prokaryotic and eukaryotic cells have in common
a plasma membrane one of more chromosomes ribosomes DNA cytoplasm
Chromosomes carry
genes made of DNA
Ribosomes make
proteins according to gene instructions
all the material inside the plasma membrane and outside the DNA containing region
cytoplasm
the fluid portion of the cytoplasm is made of
water, salts, and organic molecules
Eukaryotic cells have (prokaryotic cells don’t)
membrane-bound nucleus
number of other organelles
Prokaryotic cells have (eukaryotic cells don’t)
nucleoid no true organelles smaller and slightly different ribosomes cell wall and plasma membrane capsule/slime layer pili/fimbriae (some) flagella
the cell wall and plasma membrane maintain
call shape and protection
capsule
sticky outer coat around the cell wall of some prokaryotic cells, helping to glue it to surfaces.
flagella
propel prokaryotic cells though its liquid environment.
organelles
little organs, perform specific functions of the cell. Each organelle is bounded by a membrane with a lipid and protein composition that suits its function.
four basic functions of organelles and structures in a cell:
NUCLEUS and ribosomes are involved in genetic control of the the cell
ER, golgi, lysosomes, vacuoles and peroxisomes manufacture distribute and breakdown molecules
MITOCHONDRIA in all cells and chloroplasts in plants cells are involved in energy processing.
CYTOSKELETON plasma membrane and cell wall function as structural support, movement and communication between cells.
The internal membranes of eukaryotic cells
partition it into compartments.
cellular metabolism
occurs withing organelles
Present in animal cells, but not in plant cells are:
lysosomes and centrioles
present in plant cells but not in animal cells
cell wall (cellulose)
chloroplasts (photosynthesis)
central vacuole
plasmodesma (plasmodesmata)
plasma membrane
forms a flexible boundary between the living cell and it’s surroundings
phospholipids form a 2 layer sheet
called the phospholipid bilayer.
membrane proteins are either
attached to the membrane surface or embedded into the phospholipid bilayer.
phospholipid bilayer is the main component of
cell membranes
some proteins form channels or tunnels that
shield ions and other hydrophilic molecules as they pass through the hydrophobic center of the membrane.
some proteins serve as
pumps, using energy to actively transport molecules into or out of the cell.
membranes are composed of:
a bilayer of phospholipids with embedded and attached proteins in a structure called a fluid mosaic.
plasmodesmata
cytoplasmic channels through cell walls that connect adjacent cells
central vacuole
a compartment that stores water and a variety of chemicals.
The nucleus
contains most of the cell’s DNA and controls the cells activities by directing protein synthesis by making mRNA.
DNA is associated with many proteins in structures called
chromosomes
Proteins help
organize and coil the DNA to fit into the nucleus