BILIRUBIN

Question 1

method use in bilirubin

Answer 1

diazo method

Question 2

linear up to _____ mg/dl

Answer 2

20

Question 3

Fast incubation ______ at room temperature

Answer 3

5 minutes

Question 4

sample volume only _____

Answer 4

50 uL

Question 5

___________ is formed by the break down of RBCs in the ________, ______, _______

Answer 5

bilirubin
spleen, liver and bone marrow

Question 6

DIRECT BILIRUBIN REAGENT
1. _______ - _______
2. ________ - _______
3. ________ - ________

Answer 6

• Sulfanilic acid
• Hydrochloric acid
• preservatives and stabilizers

Question 7

DIRECT BILIRUBIN ACTIVATOR - _________

Answer 7

1x4 mL

Question 8

Nor provided along with the kit, aggape multicalibrator product code: _________is recommended

Answer 8

51610001

Question 9

The sealed reagents are stable up to the expiry date on the label, when stored at RT. The standard and activator should be stored at __________

Answer 9

Storage and stability
2-8 degrees celcius

Question 10

To avoid contamination, use clean laboratory wares. Avoid direct exposure of reagent to light

Answer 10

PRECAUTION

Question 11

serum/plasma (free of haemolysis)

Answer 11

SAMPLE

Question 12

It is recommended that each laboratory establish its own reference values. The following value may be used as guide line. ______ bilirubin is up to _______

Answer 12

direct bilirubin up to 0.4 mg/dl

Question 13

GENERAL SYSTEM PARAMETER

mode of reaction
slope of reaction
wavelength
temperature
factor (direct)
blank
linearity
reaction time
sample volume
reaction volume
activator
cuvette

Answer 13

End point
increasing
546 nm
30 C
16.0
sample blank
20 mg/dl
5 min
50 uL
1000 uL
20 uL
1 cm light path

Question 14

GENERAL SYSTEM PARAMETER
(without sample blank)

Mode of reaction
slope of reaction
wavelength 1
wavelength 2
temperature
factor (direct)
blank
linearity
reaction time
sample volume
reagent volume
activator
cuvette

Answer 14

End point
increasing
546 nm
630 nm
30 C
20.0
reagent
20 mg/dl
5 min
50 ul
1000 ul
20 ul
1 cm light path

Question 15

In the liver bilirubin is conjugated with __________ which forms a soluble compound. This is refers to __________

Answer 15

gucuronic acid
direct bilirubin

Question 16

PRINCIPLE

In _________ reacts with ___________ to form ____________. __________ reacts with ______ to form _______

Answer 16

sulfanilic acid
sodium nitrate to form sulfanilic acid direct bilirubin reacts with diazotized sulfanilic acid to form azobilirubin

Question 17

elevated levels are found in
______, _______, _____, ______,________

Answer 17

Hepatitis
cirrhosis
haemolytic jaundice
obstruction biliary tract
drug induced reaction

Question 18

Small amount of bilirubin circulates in the _______ loosely bound to ____which is not ________. This is referred to _________ or _________ bilirubin. In the liver bilirubin is conjugated with _______, which form as soluble compound . This is _______ bilirubin

Answer 18

plasma - albumin - water soluble -indirect or unconjugated bilirubin- glucuronic acid - direct bilirubin