BB Ch24 - Biohazards

Question 1

1. Which law requires employers to provide safe working conditions and empowers OSHA to promulgate standards?

Answer 1

The Occupational Safety and health Act of 1970

Question 2

2. What is the first stage in any biohazard control program?

Answer 2

To conduct a risk assessment

Question 3

3. By which route are most laboratory infections (with pathogenic agents) acquired?

Answer 3

aerosol

Question 4

4. Which particles are more likely to be inhaled – small or large?

Answer 4

4. Small particles remain airborne longer, are more likely to move with air currents, and are more likely to be respirable.

Question 5

5. What is the principle device to contain biohazards?

Answer 5

The biosafety cabinet

Question 6

6. Which class of biosafety cabinet should not be used for handling research materials that are vulnerable to airborne contaminants?

Answer 6

Class I provides no product protection

Question 7

7. Which type of class II cabinets is appropriate for microbiological research involving volatile or toxic chemicals or radionuclides?

Answer 7

Type B

Question 8

8. What are the subtypes of the type of cabinet mentioned in question 7?

Answer 8

Subtypes B1, B2, B3

Question 9

9. Describe class III cabinets

Answer 9

9. Totally enclosed, ventilated, and gas tight

Question 10

10. List some standard microbiological practices.

Answer 10

10. Wash hands thoroughly after handling animals or cultures, avoid eating, drinking, smoking, inserting contact lenses, applying cosmetics, taking medicines, touching nose, mouth eyes.

Question 11

12. How often must an eyewash station be tested?

Answer 11

monthly

Question 12

13. What can be built in an animal facility to decrease autoclave odors?

Answer 12

13. High-volume exhaust hoods that completely cover the swing area of both doors and airlocks between an autoclave and the surrounding areas.

Question 13

14. What information should be listed on biohazard signs?

Answer 13

14. The agent, investigator, room supervisor, emergency phone numbers, special requirements for entering

Question 14

15. Institutions that receive NIH support and conduct any type of recombinant DNA work must have an Institutional Biosafety Committee composed of how many members?

Answer 14

15. At least 5 members who have experience and expertise in recombinant DNA technology. (At least 2 must be unaffiliated members)

Question 15

16. Up to 56% of animal care workers are affected by which health hazard?

Answer 15

16. Animal contact allergies

Question 16

17. Tetanus toxoid injections are encouraged to be given at what intervals?

Answer 16

17. At 10 year intervals.

Question 17

18. This chapter uses the former name of “Herpes B.” What are the former and present names?

Answer 17

Former names: Herpesvirus simiae, Cercopithecine herpesvirus I. Present name: Macacine herpesvirus 1

Question 18

19. Name some zoonotic diseases that can produce teratogenic or abortifacient effects in pregnant women. Which two are the most important for pregnant women?

Answer 18

19. Brucellosis, tuberculosis, cryptococcosis, listeriosis, lymphocytic choriomeningitis, Q fever, toxoplasmosis, Venezuelan equine encephalitis. Listeriosis and toxoplasmosis.

Question 19

20. Who is responsible for presenting in the protocol about any biohazards that will be used, including radioisotopes, viable organisms, carcinogens, transplantable tumors, tissue, sera, recombinant DNA?

Answer 19

The PI