Basic Med School Knowledge + Virology Flashcards
Herpesvirus basic characteristics
DSLIDE: Double stranded, Linear, icosohedral, DNA, Enveloped.
Herpes Entrance
Viral Glycoprotein gB and gC bind to heparin sulfate. 2) gG binds to entry receptors (HVEM: herp virus entree mediator). Then gB with gH/gL fuses with GAGs.
C+D (cluster of dif required for binding). B+A+L(?) (required for fusion.
Where are you adding the Flpbow sequence? What do they code for?
Between UL26/27. Ul26: Capsid Protein. UL27: Glycoprotein B.
HRP: direction, and reference
R-retrograde. Mesulam (1982) HRP tracing handbook series
WGA-HRP
Wheat Germ Agglutinin HRP: Retrograde: gets uptaken in lysosome (HRP does as well)
PHA-L
Phageolus Vulgaris Leucoagglutinin (PHA-L): A-anterograde
TTC
Tetanus Toxin Fragment c: Retrograde
Viruses used for Tracing
Retro: PRV (Alpha Hepre), Rabies (Rhado)
Antero: HSV, VSV (Rhabdo), Sindbis (toga)
AAV: Parvo “antero”
Parvovirus
NESSLD: Nonenvleoped, single stranded, linear, DNA
What deletions prevent spread of PRV into axons. Source (Recent review with enquist)
PRV Bartha lacks: gE, gI and US9. Matt Taylor +Enquist 2015. Trends in Microbiology)
Rhadobvirdae different than HSV?
HSV/PRV assembled in cell body. RV pieces are carried down axon/dendrites and assembled before egress
WPRE. Paper and Mechanism.
Donello Hope 1999 Jour of Virology. Tested GFP and luciferase expression and found a 5-8x increase in expression.
Possible Mechanism: introns have regualtory sequences,. Splicing seems to stim gene expression (Ryu and Mertz 1989).
PRV-IE180 Null Paper. Length of time between PRV’s
mbio 2014: Wu, Engel, Enquist.
PRV 151: 10 days showed signs of infection (in vitro)
PRV 128: no effects 1 month after.
Showed no superinfection exclusion.
Rabies papers for retrograde spread
Ugolini (1995, 2010); Callaway 2008)
SAD-Delta-G Rabies (both papers) and what they made
Wickersham et al 2007: Replaced Glycoprotein with EGFP. Transcomplementation of G-protein, and psuedotyped virus for Avian env A (so works with TVA now).
Osakada et al 2011 Neuron: made variants including Tet inducible cre, tamoxifen indcubile cre, and flippase expressing cre systems. Showed no infection sxs post 7 days but dead at 14 (window is 5-11 days).
PRV paper. Where does PRV diffuse? PRV replication?
Gary Aston-Jones, Card 2000. PRV has affinity for ECM (such as Hep suflate) so doesn’t diffuse much. CAN infect perikarya, but affinity is for axon terminals.
Paper showing PRV with CTB. Author, what did it show
O’Donnell 1997. Showed CTB zone is larger than uptake of virus
When and where was H129 cultured from? What paper did the neurovirulence analysis on it?
Taken from a Herpes encephalitis case in 1977. Dix et al 1983 did the virulence assay and found it to be anterograde
PRV Bartha deletions normally necessary for anterograde spread
gE, gI (both used at axon terminal, possibly for egress?)
and US9 needed for sorting at the axon.
All are necessary for anterograde. Bartha is missing all 3..
Enquist 2002.
Number of viral genomes per cell? Paper?
Less than 7. Kobiler (enquist) 2010
Original Brainbow Paper
Livet….Sanes & Lichtman 2007
