Basic Immunology Flashcards
What are the 2 main categories of the immune system?
Innate and adaptive
Explain the differences in
- resonse time
- specificity, memory, response to repeat infection, cellular components and humeral components
Innate: Response hours, limited specificity, no memory, identical response to repeat infection, phagocytes/NKC, complement pathway
Adaptive: réponse days, diverse specificity, efficient memory, in repeat infection much more rapid and vigorous response, T cells/Bcells, antibodies
What are the main types of phagocytes?
- Monocyte/macrophage lineage
- granulocytes (neutrophils, mast cells, basophils and eosinophils
What is the most abundant type of leukocyte?
Neutrophils
How are phagocytes routinely activate?
Attracted to chemokine and activate by cytokines that are secreted by antigen specific T cells
How do NK cells detect tumour cells to virally infected cells?
CD16 receptor (FcyRIII) - attaches to specific antibodies bound to tumour cells/virally infected cells KIRs (killer cell immunoglobulin like receptors) - interacts with MHC class 1 on target cells
Describe the 3 ways in which the complement pathway can be activated (both adaptive & innate.
- Classic pathway (Antigen-antibody complex) adaptive immunity
- Alternative pathway (C3b bind to hydroxyl/amine group on surface of pathogen) Innate
- Lectin pathway (lectin binds to glycoproteins + carbohydrates on surface of pathogens)
Briefly describe the complement pathway
The most abdundant complement C3 is activated by either of the 3 pathways, all coverage as C5-C9 interact to form a membrane attack complex which binds to the membrane of target cells → transmembrane channel salt & water can flow = lysis
Where are B cells developed?
Bone marrow
Briefly describe the activation of B cells
Antigens bind to antibodies on the surface of the naive B cells. They are internalised and complex with MHC class II and returned to the cell surface.
Antigen-specific T cells recognise these and secrete cytokines causing clonal B cell proliferation and differentiate into plasma cells which produce antibodies.
What are the 5 main types of antibodies?
IgM, IgG, IgA, IgE, IgD
What is the basic structure of antibodies?
2 identical light chain
2 Identical heave chains
Differ in size, charges carbohydrate content and amino acid sequence.
Antigen binding fragment (fab) region → antibodies antigen specificity
Fc region → effector function
For IgM describe:
- % of serum immunglobin pool
- Shape
- Role in primary/secondary response?
- Can it cross the placenta?
- what is its effector function
- 5-10%
- pentamer
- 1st antibody in primary response
- Can not cross placenta, 1st antibody the newborn baby produces.
- potent activator of complement pathway due to 4 complement binding pathway
For IgG
- % of serum immunglobin pool
- Role in primary/secondary response?
- Can it cross the placenta?
- what is its effector function
- 70-80%
- predominant in Secondary immune responses not primary
- Can cross into placenta
- Activate complement and bind to phagocytes
For IgA
- % of serum immunglobin pool
- Where is it abundant?
- Role in primary/secondary response?
- Can it cross the placenta?
- what is its effector function
- 10-20%
- Salivia, tears, mucus, breast milk
- Important in secondary immune response
- No but secreted in breast milk important for fetes in first few months of life
- Induce phagocytosis, prevent attachment to mucosal cells
For IgE
- High affinicty for which cells
- what is its effector function
- Mast cells and basophils
- Degranulation release of active mediators, allergic manifestations
How does vaccination improve future secondary responses to infection.
As vaccination expose the immune system in an inocus antigen, this produces primary antibody response and leads to generation of long lived antigen specific memory B cells.
Subsequent expose to same pathogen leads to faster more intense secondary response.