Bacterial Growth Flashcards

0
Q

What is agar derived from?

A

Seaweed

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1
Q

What is the term for: a population of organisms descended from a single cell and it is therefore separated from all other species?

A

Pure culture

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2
Q

Who developed solid media?

A

Koch

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3
Q

Who invented the dished that agar is kept in? And was is the name of the dish?

A

Kochs lab ass, Julius Richard Petri

Petri dish - duh.

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4
Q

Define binary fission:

A

After a bacterial cell has increased in size and double all of its parts it divides.

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5
Q

When are bacterial chromosomes replicated?

A

Early in cells life

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6
Q

What does a cell do to induce division?

A

Grows and elongates to about twice its size.

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7
Q

What happens to DNA during binary fission? (This is a weird question and answer…sorry)

A

DNA is partitioned, cell lays down new plasma membrane and cell wall materials in region between attachment site (septum) of two daughter genomes

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8
Q

What happens to the new plasma membrane in binary fission?

A

Eventually grows all the way around separating cell, lastly cell wall grows.

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9
Q

What is microbial growth defined as being?

A

An increase in the number of cells in a population

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10
Q

What is the generation time?

A

Time is takes for a population to double in number.

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11
Q

What is the growth curve?

A

In a closed system the population growth follows a pattern of stages, this is called the growth curve.

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12
Q

Define lag stage:

A

Cells “ramp up” by synthesizing macromolecules required for division, and ATP.

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13
Q

What is the term for the following? Cells dividing at a constant rate, growth is balanced with available nutrients

A

Exponential phase (log)

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14
Q

Define stationary phase:

A

Cells have exhausted supply of energy and nutrients, number of cells remains relatively constant

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15
Q

Define death phase

A

Population decreases as cells die off at constant rate

16
Q

What are the two methods to measure growth?

A

1) direct count method using microscope and hemacytometer

2) indirect cell count using serial dilution