Bacterial DNA replication and partitioning

Question 1

what are the following abbreviations
bp
kb
Mb
Gb

Answer 1

base pair
1000 bp
1,000,000 bp
1,000,000,000 bp

Question 2

How big is human genome

Answer 2

3 Gb

Question 3

How big is bacterial genome

Answer 3

08-13 Mb

Question 4

what is horizontal gene transfer

Answer 4

happens when genetic information is passed sideways from a donor to an unrelated organisms

usually involves plasmids, viruses, or transposons

Question 5

What is e coli? Gram + or Gram -

Answer 5

Gram -

Question 6

Bacterial nucleoid

Answer 6

fileld with bacteria chromosomes

Question 7

where are ribosomes and transcription machinery in bacteria?

Answer 7

the outside fo the nucleoid because of their size

Question 8

What two levels of compaction are essential to chromosome

Answer 8

supercoiled and folded into ~30 ~300 topologically isolated domains that are arranged around a protein scaffold

Question 9

are nucleoid placements random?

Answer 9

no, they are positioned specifically

Question 10

where are highly transcribed genes located

Answer 10

the periphery of the nucleoid

Question 11

what bends DNA

Question 12

What is supercoied?

Answer 12

DNA negatively supercoiled

Question 13

what is the form of DNA

Answer 13

right handed super helix

Question 14

what does negative supercoiling do?

Answer 14

underwinds it and facilitates the formation of bubbles

Question 15

What does positive supercoiling do?

Answer 15

overwinds

Question 16

Topoisomerave IV does what

Answer 16

works behind the replication form and untangles the newly formed DNA as replication proceeds

Question 17

what is OriC

Answer 17

DNA replication stats at this cite and proceeds in both directions around the circle

Question 18

What enzymes are important in bacteria replication

Answer 18

DnaA, DnsB, DnaC, DNA gyrase, SSB

Question 19

Hemimethylated

Answer 19

the old strand is methylated but the new strand has not yet been methylated

Question 20

what happen if hemimethylated

Answer 20

can not replicate again???????

Question 21

what does asymetric distribution of DNA do?

Answer 21

aids in the organization and segregation of the genome

Question 22

what does Tus do?

Answer 22

binds symmetrically and stops the movement of the form from one direction only

Question 23

what is a septum?

Answer 23

a cross wall between two daughter ceolls

Question 24

what is partitioning?

Answer 24

segregation

Question 25

What sequences help to mediate partitioning?

Answer 25

ParA, ParB and specific sequences near the origin of replication called parS

Question 26

Genome segregation. How work?

Answer 26

ParA

Question 27

Catenated what mean

Answer 27

interlocked

Question 28

what does topoisomerase IV do?

Answer 28

separated catenanes

Question 29

What does Ftsk stand for

Answer 29

Filamentous temperature sensitive

Question 30

what does Ftsk do?

Answer 30

translocates DNA through the septum at 7 kb/sec and places two dif sites in the middle of the dividing cell

Question 31

what is a divisom

Answer 31

proteins that assemble together to create the future division site

Question 32

FtsZ

Question 33

Generation time

Answer 33

you got that.
10º Hotter make things take half the time.