Bacteria, Viruses, Fungi + Yeast Flashcards
To which kingdoms do amoeba and bacteria belong?
amoeba - protista
bacteria - monera
which kingdom contains eukaryotic organisms between monera and Protista.
protista
Comment on the relationship between the mode of nutrition and the environmental roles of a saprophyte
saprophytes consume dead matter and are therefore reponsible for decomposition and nutrient recycling in the environment.
In the sexual reproduction of rhizopus, what is the significance of opposite strains of hyphae growing close together ?
The swellings form and grow towards each other and eventually touch.
In the sexual reproduction of rhizopus, describe the production progametangia.
This is the swellings at the tip of the hyphae.
In the sexual reproduction of rhizopus, what is the role of meiosis?
The zygospore germinates by meiosis.
State any 2 harmful effects of fungi?
Human disease such as athletes foot and ringworm.
Poisonous mushrooms
What are the circumstances under which sexual reproduction of the rhizopus occurs?
Lack of water
unsuitable temperature
unsuitable pH
Lack of nutrients
Is oxygen necessary during fermentation ?
No
Where in the cells does fermentation occur ?
cytosol
When preparing alcohol using yeast, from which substance did the yeast make the alcohol?
Glucose
When preparing alcohol using yeast, what is the optimum temperature and how was it maintained?
20C - 35C
using a water bath
When preparing alcohol using yeast, How did you know when the fermentation was finished ?
No more carbon dioxide was produced
When preparing alcohol using yeast, how did you test for the presence of alcohol and what is the colour of the positive result ?
Using the iodoform test
yellow
State the 2 sources of energy that bacteria can use make their food
Sunlight or energy from chemical reactions.
What is the difference between facultative anaerobes and obligate anaerobes ?
facultative respire in the presence or absence of oxygen while obligate respire only in the absence of oxygen.
In the growth curve of a bacteria, explain why some of them survive ?
They form endospores
During continuous flow batch processing, explain why its desirable to keep bacteria in the log phase of growth
To maximise yield
During continuous flow batch processing, at what stage of growth curve is the product removed ?
After stationary phase
To what kingdom do yeasts belong ?
Name another member of this kingdom
fungi
mushrooms/ rhizopus/ penicillium
Apart from a source of nutrition, give one other function of the agar.
Its a medium for growth
In relation to the growth of leaf yeasts in the lab, how can you be certain that the leaf is the only possible source of the yeast ?
You can use a sterilised piece of leaf as a control
In relation to the growth of leaf yeasts in the lab, what is the step taken to prevent the contamination of the lab by the yeast ?
seal the plates
In relation to the investigation of anaerobic respiration in yeast, name 2 steps taken to ensure anaerobic conditions.
Boil water then cool it to remove all oxygen.
Add layer of oil to prevent oxygen from entering.
In relation to the investigation of anaerobic respiration in yeast, what visible sign is there that fermentation is taking place ?
Bubbles of gas of carbon-dioxide.
In relation to the investigation of anaerobic respiration in yeast, if the apparatus was placed in an ice-bath, what effect would this have on the rate of respiration ?
it slows it down because the rate of enzyme action is reduced by the decrease in temperature.
Give an example of the beneficial effects of bacteria, aside from the production of antibiotics, and one example of a harmful effect of bacteria.
A beneficial effect of bacteria is decomposition.
A harmful effect of bacteria is food decay.
What is the method of asexual reproduction of bacteria called ?
Binary fission
Describe the process of binary fission.
The DNA replicates. The cell then elongates and each DNA moves to opposite sides of the elongated cell. A cell membrane grows in between them and the cell therefore splits into two.
Why is the overuse of antibiotics dangerous ?
Because it can lead to the development of antibiotic resistant bacteria.
How do certain bacterias survive in unfavourable conditions ?
They form an endospore
Name a fungus other than yeast that you have studied in your course.
Give one way this fungus differs from yeast
rhizopus
It is multicellular and it produces zygospores
In relation to the growth of leaf yeasts in the lab, mention 2 aseptic techniques you carried out to ensure a pathogen- free environment.
sterilising instruments
washing hands or wearing gloves
disinfecting bench and equipments
What type of agar is recommended for optimal growth of yeast and other fungi ?
malt agar
nutrient agar
In relation to the growth of leaf yeasts in the lab, describe how the plates were stored, from introduction of the yeast source until yeast growth was visible on the agar.
They were first stored right-side up for the first 1 or 2 days. Then they were stored upside down in an incubator for 2-7 days.
In relation to the growth of leaf yeasts in the lab, how did you identify the leaf yeast growing on the agar ?
They were present in pink colonies
In relation to the growth of leaf yeasts in the lab, suggest a reason why few or no leaf yeasts may have grown on the agar.
unsuitable temperature
no yeast on leaf
air pollution
State a feature of eukaryotic organisms
They have membrane-bound organelles
Give an example of a unicellular and a multicellular fungus
A unicellular fungus is yeast
A multicellular fungus is rhizopus
To which group of biomolecules does chitin belong ?
carohydrates
What is the type of nutrition found in fungi ?
saprophytic - they feed of dead matter or heterotrophic - they do not make their own food.
What is the importance of a decomposer ?
They recycle nutrients or minerals
What is the purpose of budding in fungi ?
reproduction
What part of the bacteria cell is responsible for protection ?
the capsule
What part of the bacteria cell is responsible for drug resistance ?
the plasmid
Name an organelle that is present in plant cells but absent in bacterial cells
mitochondria
chloroplasts
What is the role of an endospore ?
allows the bacteria to survive in unfavourable conditions
Explain the term chemosynthetic ?
This is the use of energy released from chemical reactions
Give an example of a chemosynthetic bacteria
Nitrifying bacteria
In relation to the growth of leaf yeasts in the lab, was the investigation carried out in a sterile or aseptic environment ?
Explain your answer
Aseptic
This means not all microbes were destroyed
What is the meaning of asepsis ?
This means free of pathogens
What is a pathogen ?
This is a disease causing organism
What is an antibiotic ?
This is a chemical produced by micro organisms to kill or stop the growth of other micro organisms
What is bioprocessing ?
This is the use of micro-organisms to form products
What is a bioreactor ?
This is a vessel in which products are made by micro organisms. it is vessel in which bioprocessing occurs.
Define osmosis
This is the movement of water through a semi permeable membrane from a high water concentration to a low water concentration