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Semester 3 > Bacteria Identification > Flashcards

Bacteria Identification Flashcards

1
Q

If a Bacteria does not stain Gram Positive/Negative , what are the possible classes of Organisms?

A

Obligate Intracellular

Spirochetes

Mycoplasmas (Mollicutes)

Acid Fast (Myobacteria)

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2
Q

If Gram Positive Cocci Shaped Bacteria is found to be Catalase Positive , what species could it be and what would the next appropriate examination?

A

Any Staphylococci Bacterium ( Facultative Anaerobes)

Staphylococcus Aureus

Staphylococcus Epidermidis

Staphylococcus Saprophyticus

Next test would be a Coagulase Exam ( Fibrin Formation)

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3
Q

If a Gram Positive Bacterium is Catalase + , and Coagulase + , what species has been identified?

A

Staphylococcus Aureus

Normal Flora on skin , in nares

Colonies often yellow

Beta Hemolytic

Salt Tolerant ( Mannitol Salt Agar)

Produce Acid From Mannitol ( Red to Yellow)

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4
Q

If a Gram Positive Cocci is catalase + , and Coagulase Neg , and susceptible to Novobiocin, what are the possible species of bacterium?

A

Normal Flora on skin , nares and vagina

Non Hemolytic

S epidermidis

Novobiocin Suceptible

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5
Q

If a Gram Positive Bacteria is:

Catalase Positive

Coagulase Negative

Cocci

Novobiocin Resistant

What is the species?

A

S saprophyticus

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6
Q

What species of bacterium are Gram Positive and Catalase Negative?

A

Streptococci/Enterococci

Anaerobes

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7
Q

If a bacterium is found to be:

Gram Positive Cocci

Catalase Negative

Alpha Hemolytic

Optochin Susceptible

What is the Identity?

A

Streptococcus Pneumoniae

Aerotolerant

Bile Soluble

Diplococci

No Lancefield Antigen Result

Normal Pharyngeal Flora

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8
Q

If a Bacterium is found to be:

Gram Positive Cocci

Catalse Negative

Alpha Hemolytic

Optochin Resistant

Bile Resistant

What is the Identity of the Organism?

A

Viridans Streptococci

Aerotolerant

Normal Oral Flora

S mitis

Various Lancefield Groups

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9
Q

If a Bacterium is found to be:

Gram Positive Cocci

Catalse Negative

Beta Hemolytic

Bacitracin Susceptible

What is the species?

A

Streptococcus Pyogens

Aerotolerant

PYR +

Lancefield Group A

Can be part of normal pharyngeal flora

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10
Q

If a Bacterium is found to be:

Gram Positive Cocci

Catalse Negative

Beta Hemolytic

Bacitracin Resistant

What is the the Species?

A

Streptococcus Agalactiae

Aerotolerant

PYR -

Lancefield Group B

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11
Q

If a Bacterium is found to be:

Gram Positive Cocci

Catalase Negative

Non Hemolytic (Y)

What is the species?

A

Enterococcus

Some Aerotolerant

Lancefield Group D

Black Colonies on Bile Esculin Agar

Esculin and Esculitin

Black Precipitate

*******SOME STREPTOCOCCUS WONT GROW ON OR WILL BE CLEAR OF ESCULIN AGAR********

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12
Q

If a Bacterium is found to be:

Gram Positive Bacilli

Endospore Forming

Catalase Positive

What is the species?

A

Bacillus

Soil and Water

Aerobe

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13
Q

If a Bacterium is found to be:

Gram Positive Bacilli

Endospore Forming

Catalase Negative

What species is present?

A

Clostridium

Found in soil , water , GI tract

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14
Q

If a Bacterium is found to be:

Gram Positive Bacilli

Non Endospore Forming

Aerobe

Club Shaped

What Species is present?

A

Corynebacterium Diphtheriae

Normal Part of Skin, Vaginal , Pharyngeal flora

Toxigenic Strains cause disease

Chinese Letter appearance

Brown Black Colonies on Tellurite Agar

Loeffler Agar coloines are white , cells have polar metachromatic granules

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15
Q

If a Bacterium is found to be:

Gram Positive Bacilli

Non Endospore Forming

Aerobes

Low Growth Temperatures as Low as 2 Degrees

What species is present?

A

Listeria

Tumbling Motility in Broth

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16
Q

If a Bacterium is found to be:

Non Endospore Forming

Gram Positive Bacilli

Aerobes Partial Acid Fast

What Species is present?

A

Nocardia

Filamentous Branching

Found in Soil and Water

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17
Q

If a Bacterium is Found to be:

Non Endospore Forming

Anaerobe

Gram Positive Bacilli

Acne

What Species is present?

A

Propionibacterium

Chains or clumps , short rods

Common on skin , vagina and oropharynx

Opportunistic Infection

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18
Q

If a Bacterium is found to be:

Gram Positive Bacilli

Anaerobe

Catalase Neg

Strict or Aerotolerant

Endocarditis

What Species is present?

A

Lactobacillus

Part of Normal Flora

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19
Q

If a Bacterium is found to be:

Gram Positive Bacilli

Non Endospore Forming

Anaerobe

Catalase Neg

Knot up into yellow flecks visible in pus

What Species is Present?

A

Actinomyces

Filamentous Branching

Part of normal oral , GI Flora

Cervicofacial Infection Most Common

20
Q

If a Bacterium is Found to be:

Gram Negative Cocci

Oxidizes Glucose

Oxidase Positive

Catalase Positive

Found on Choclate Agar

What Species is Present?

A

Neisseria Gonorrhoeae

Diplococci

PMNs in direct smears

Aerobes but use CO2

Non Motile

Some Found in Throat

21
Q

If a Bacterium is Found to be:

Gram Negative Cocci

Diplococci

Oxidize Glucose and Maltose

Some Species normal in Throat

What Species is Present?

A

Neisseria Meningitidis

Infects Blood and CNS

Non Motile

Aerobes

Often Associates with PMNs

22
Q

If a Bacterium is Found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorph

Aerobe (Non Fermenter)

Oxidase Negative

Catalase Positive

What Species is Present?

A

Acinetobacter

Cocci or Rods

Normal Oropharyngeal Flora

Soil/Plants

Easy to Grow on Blood Agar

23
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorph

Aerobes (Non Fermenter)

Oxidase and Catalase Pos

What Species is Present?

A

Moraxella

Diplococcus

Normal Respiratory Flora

Blood Agar

24
Q

If a Bacterium is Found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorphs

Aerobes ( Non Fermenter)

Oxidase Neg

3 Day Incubation

What Species is Present?

A

Francisella Sp.

Very Small , Faint Staining

BCYE or Choclate Agar + Cys

Serology Testing

25
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorphs

Aerobes (Non Fermenter)

Oxidase Pos

What Species is Present?

A

Brucella Sp.

Small Intracellular

Enriched Blood Agar

3 Days +

Serology

26
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorphs

Aerobes (Non Fermenter)

Bordet Gengou , Regan Lowe Agar

4 to 12 dat incubation

What Species is Present?

A

Bordetella Sp.

PCR often used

Oxidase Variable , motility variable

27
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacilli/ Pleomorphs

Facultative Anaerobe

Requires Factor X and Factor V

What Species is Present?

A

Haemophilus

Often Seen as a Rod , can grow as long as a filament

Choclate Agar

Oxidase Pos and Neg

28
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorph

Facultative Anaerobes

Bipolar Staining

What Species is Present?

A

Pasteurella

Oxidase Positive

29
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorph

Anaerobes

Stain Poorly

Can be motile

What Species is Present

A

Bacteroides

Pleomorphic

Easy to Grow

Normal GI , Oral Flora

30
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacili/Pleomorph

Anaerobes

Normal Oral Flora

What Species is Present?

A

Prevotella

Very Small

Slow Growing Fastidious

Black Colonies on Bap

31
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Coccobacilli/Pleomorphs

Anaerobes

Black Colonies of BAP

What Species is Present?

A

Porphyromonas

Very Small

Slow Growing Fastidious

Normal Oral Flora

32
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Curved and Spiral Rods (Motile)

Aerobe

Gull Wing

Grow at 42 Degrees

What Species is Present?

A

Campylobacter Sp.

Oxidase Positive

Motile

MicroAerophilic

33
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Curved and Spiral Rods (Motile)

Aerobes

Oxidase and Urease Positive

What Species is Present?

A

Helicobacter Sp.

Microaerophilic

34
Q

If a Bacterium is found to be:

Gram Negatice Bacilli

Curved and Spiral Rods (Motile)

Facultative Anaerobe

Alkalophile

Like Salt

What Species is Present?

A

Vibrio Sp.

Comma Shaped Rods

Oxidase Positive

Motile

35
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase Negative

Ferment Glucose

What Species is Present?

A

Enterobacteriaceae

Normal GI Flora

Medical Importance

Serratia can be Lac +/- often produces red pigment

36
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase Negative

Lactose Fermenting (CEEK)

Urease and H2S (+/-)

What Species is Present?

A

Citrobacter

Motile

37
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase Negative

Lactose Fermenting (CEEK)

Urease and H2S (+/-)

What Species is Present?

A

Enterobacter

38
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase Negative

Lactose Fermenting (CEEK)

Urease and H2S Negative

Often Encapsulated

What Species is Present?

A

Escherichia

39
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase Negative

Lactose Fermenting (CEEK)

Urease(+) and H2S(-)

Non Motile

Often Highly Mucoid

What Species is Present?

A

Klebsiella

40
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase (-)

Lactose Non Fermenting (ShYPS)

Non H2S Producing

Urease Negative

What Species is Present?

A

Shigella

Non motile

41
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase (-)

Lactose Non Fermenting (ShYPS)

Non H2S Producing

Can grow at less that 4 Degrees

What Species is Present?

A

Yersinia

Bipolar Staining

Slower Growing

Coagulase (+/-)

Urease (+/-)

Motility (+/-)

42
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase (-)

Lactose Non Fermenting (ShYPS)

H2S Producing

Swarming Motility

What Species is Present?

A

Proteus

Urease +

43
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Facultative Anaerobes

Oxidase (-)

Lactose Non Fermenting (ShYPS)

H2S Producing

Urease Negative

What Species is Present?

A

Salmonella

Motile

44
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Aerobes

Oxidase (+)

Non Fermenters

Resist Cetrimide

Multi Drug Resistant

What Species is Present?

A

Pseudomonas Sp.

Beige on MacConkey

Produce Pyocyanin

Can Grow Anaerobically with Alternate Electron Acceptors

45
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Aerobes

Oxidase (+)

Non Fermenters

Silver Stain

Direct Fluorescents Antibody (DFA)

What Species is Present?

A

Legionella Sp.

Thin , short or long

Gram Stain Poorly

BCYE Agar + Cysteine , Iron

3-5 Days incubation

46
Q

If a Bacterium is found to be:

Gram Negative Bacilli

Straight Rods

Anaerobes

Thin and Pointy

What Species is Present?

A

Fusobacterium

Semester 3 (6 decks)

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