Bacteria

Question 1

Listeria Monocytogenes Gram

Answer 1

GPB

Question 2

Listeria monocytogenes appearance on:
- LIST plate
- CBA

Answer 2

LIST: black colonies
CBA: like a GBS (haemolytic grey cols, catalase pos)

Question 3

Aggregagatibacter actinomycetemcomitans Gram

Answer 3

GNC / cocco-baccilli

Question 4

Cardiobacterium hominis Gram

Answer 4

pleomorphic GNB

Question 5

Eikinella corrodens Gram

Answer 5

GNB

Question 6

Kingella sp Gram

Answer 6

GN cocco-baccilli

Question 7

Pasteurella multicoda Gram

Answer 7

GNB - safety pin appearance with bipolar staining

Question 8

Pasteurella sp appearance on CBA

Oxidase?
Catalase?

Answer 8

coliform-like
Ox pos
Cat pos

Question 9

What organisms are associated with animal bites?

Answer 9

Pasteurella canis
Capnocytophaga canimorsus

Question 10

Actinomyces sp Gram

Answer 10

GPB branching with t-shaped jointed

Question 11

Actinomyces sp on ACTINO agar

Answer 11

bread-crumb colonies

Question 12

What organisms can cause erysipelas?

Answer 12

Group A strep

sometimes: SAUR, Group G strep

Question 13

What organism causes Erysipeloid (hot swollen red/purple plaques, often on hands of farmworkers, vets, butchers)?

Answer 13

Erysipelothrix rhusiopathiae

(short slim GPB)

Question 14

Erysipelothrix rhusiopathiae Gram and appearance on agar

Answer 14

short slim GPB

small AHS, slow growing, anaerobic

Question 15

What three organisms are important causes of necrotising fasciitis?

Answer 15

group A strep
Vibrio vulnificus
Clostridium perfringens

Question 16

Clostridium perfringens Gram

Answer 16

big sturdy GPB

Question 17

C perfringens on FAA

Answer 17

Double zone of haemolysis, umbonate wavy edged colonies, MTZ S

Question 18

What organism is associated with gas gangrene?

Answer 18

C perfringens

toxin release creates gas

Question 19

N gonorrhoeae Gram

Answer 19

GNDC

Question 20

Coryebacterium diptheriae Gram and appearance on Hoyles plate?

Answer 20

small GPB in palisades

black colonies

Question 21

When do we add a CHOC plate to throat swabs?

Answer 21

clinical details: epiglottitis
target org = HINF

Question 22

Causative organisms of vincent’s angina/trench mouth?

Presents with pharyngeal and gum ulceration

Answer 22

Fusobacterium necrophorum and Borrelia vincentii

Question 23

F necrophorum Gram and appearance on agar

Answer 23

Gram: GNB, sometimes in chains

B-haem colonies, MTZ S, pointy colonies, yellow-ish colour

Question 24

Arcanobacterium haemolyticum Gram and appearance on agar

Answer 24

small GPB, sometimes branching

B-haem strep - non-grouping

Question 25

What rash is caused by Mycobacterium marinum?

Answer 25

Fishtank granuloma

Question 26

Mycobacterium sp gram appearance?

Other microscopy techniques to identify Mycobacterium?

Answer 26

Gram variable beaded branching rods - poorly stained

Auramine staining - Mycobact’ fluoresce yellow-green

Zeihls-Neelson stain - pink/purple can see the morphology more clearly

Question 27

Treponema pallidum morphology and causes which disease?

Answer 27

Cannot be seen in Gram

Can be seen on dark field -tightly wound spirochaete

Syphilis

Question 28

Ureaplasma urealyticum causes what?

How can it be detected?

Answer 28

genital tract infections in men and women

PCR

Question 29

Mycoplasma hominis and Ureaplasma urealyticum are detected using PCR, why?

Answer 29

Both are difficult to grow and identify on agar. They do not have cell walls.

Ureaplasma sp are extremely small.

Question 30

What can L monocytogenes cause in pregnancy?

Answer 30

maternal septicaemia, morbidity/mortality of fetus